Marine enzymes and specialized metabolism
Author(s)
Bibliographic Information
Marine enzymes and specialized metabolism
（Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan, v. 604-605）
Academic Press, Elsevier, 2018
1st ed
- pt. A
- pt. B
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Note
Includes bibliographical references
Description and Table of Contents
- Volume
-
pt. A ISBN 9780128139592
Description
Marine enzymes and specialized metabolism - Part A, Volume 604 highlights experimental methods on diverse marine enzymes involved in the construction of bioactive natural product molecules. These detailed protocols are written by experts who actively study and apply marine enzymes in biosynthesis and biotechnology. Comprehensive chapters in this latest release cover Chemoenzymatic synthesis of starting materials and characterization of halogenases requiring acyl carrier protein tethered substrates, Assaying biradical aryl coupling activity of CYP450 enzymes, the Characterization and application of marine microbial omega-3 polyunsaturated fatty acid synthesis, Catalase-related allene oxide synthase: on a biosynthetic route to fatty acid cyclopentenones, Haloalkane dehalogenases from marine microorganisms, and more.
Table of Contents
Section I: Marine Cyanobacteria, Enzymes and Pathways 1. Collection, Culturing, and Genome Analyses of Tropical Marine Filamentous Benthic Cyanobacteria Nathan A. Moss, Tiago Leao, Evgenia Glukhov, Lena Gerwick and William H. Gerwick 2. PKS-NRPS Enzymology and Structural Biology: Considerations in Protein Production Meredith A. Skiba, Finn P. Maloney, Qingyun Dan, Amy E. Fraley, Courtney C. Aldrich, Janet L. Smith and W. Clay Brown 3. Terminal Alkyne Biosynthesis in Marine Microbes Xuejun Zhu and Wenjun Zhang 4. The Biochemistry and Structural Biology of Cyanobactin Biosynthetic Enzymes Wenjia Gu, Shi-Hui Dong, Snigdha Sarkar, Satish K. Nair and Eric W. Schmidt 5. The Enzymology of Prochlorosin Biosynthesis Silvia C. Bobeica and Wilfred A. van der Donk
Section II: Biosynthesis by Bacterial Symbionts of Marine Invertebrate Animals 6. Chemoenzymatic Dissection of Polyketide ss-Branching in the Bryostatin Pathway Samuel T. Slocum, Andrew N. Lowell, Ashootosh N. Tripathi, Vikram V. Shende, Janet L. Smith and David H. Sherman 7. Radical S-Adenosylmethionine Peptide Epimerases: Detection of Activity and Characterization of d-Amino Acid Products Brandon I. Morinaka, Anna L. Vagstad and Joern Piel 8. Cobalamin-Dependent C-Methyltransferases From Marine Microbes: Accessibility via Rhizobia Expression Michael F. Freeman 9. Biosynthetic Insights of Calyculin- and Misakinolide-Type Compounds in "Candidatus Entotheonella sp." Agustinus R. Uria, Joern Piel and Toshiyuki Wakimoto
Section III: Natural Product Biosynthetic Enzymology of Marine Bacteria and Fungi 10. Chemoenzymatic Synthesis of Starting Materials and Characterization of Halogenases Requiring Acyl Carrier Protein-Tethered Substrates Hem R. Thapa, Andrew J. Lail, Neha Garg and Vinayak Agarwal 11. Preparation, Assay, and Application of Chlorinase SalL for the Chemoenzymatic Synthesis of S-Adenosyl-L-Methionine and Analogs Tony D. Davis, Sylvia Kunakom, Michael D. Burkart and Alessandra S. Eustaquio 12. In Vitro Analysis of Cyanobacterial Nonheme Iron-Dependent Aliphatic Halogenases WelO5 and AmbO5 Xinyu Liu 13. Characterization and Biochemical Assays of Streptomyces Vanadium-Dependent Chloroperoxidases Shaun M.K. McKinnie, Zachary D. Miles and Bradley S. Moore 14. Unusual "Head-to-Torso" Coupling of Terpene Precursors as a New Strategy for the Structural Diversification of Natural Products Robin Teufel 15. Identification of Enzymes Involved in Sesterterpene Biosynthesis in Marine Fungi Jingjing Yan, Jingjing Guo, Wei Yuan, Wanying Mai and Kui Hong 16. Assaying Oxidative Coupling Activity of CYP450 Enzymes Vinayak Agarwal 17. Preparation and Characterization of the Favorskiiase Flavoprotein EncM and Its Distinctive Flavin-N5-Oxide Cofactor Robin Teufel
- Volume
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pt. B ISBN 9780128150450
Description
Marine enzymes and specialized metabolism - Part B, Volume 605 in the Methods in Enzymology series, highlights experimental methods on diverse marine enzymes involved in the construction of bioactive natural product molecules. Unique sections in this new release include discussions on polysaccharide-degrading enzymes from marine gastropods, radical SAM epimerases from sponge microbes, DMS/P demethylase in bacteria, reconstitution of particulate methane monooxygenase into membrane mimetics, the structure and function of cyanobactin enzymes, marine cyanobacterial polyketide beta-branching enzymology, marine cyanobacterial PKS-NRPS enzymology and structural biology, biochemical profiling of DMSP lyases, and more.
Table of Contents
1. Polysaccharide-degrading enzymes from marine gastropods Takao Ojima 2. Radical SAM epimerases from sponge microbes Joern Piel 3. DMS/P demethylase in bacteria Chris Reisch 4. Reconstitution of particulate methane monooxygenase into membrane mimetics Amy C. Rosenzweig 5. Structure and function of cyanobactin enzymes Eric W. Schmidt 6. Marine cyanobacterial polyketide beta-branching enzymology David Sherman 7. Marine cyanobacterial PKS-NRPS enzymology and structural biology Janet Smith 8. Biochemical profiling of DMSP lyases Dan Tawfik 9. Preparation and characterization of the enterocin favorskiiase flavoprotein EncM and its distinctive flavin-N5-oxide cofactor Robin Teufel 10. Unusual "head-to-torso"-coupling of terpene precursors as a new strategy for the structural diversification of natural products Robin Teufel 11. Soluble proton ATPases from marine organisms Martin Tresguerres 12. Prochlorosin enzymology in marine cyanobacteria Wilfred van der Donk 13. The Biosynthetic Insights on MIsakinolide and Calyculin in Candidatus 'Entotheonella' Toshiyuki Wakimoto 14. Algal vanadium-dependent haloperoxidases Ron Wever 15. Methane biosynthesis and phosphonates in pelagic bacteria David Zechel 16. Terminal alkyne biosynthesis in marine microbes Wenjun Zhang
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