Cell senescence : methods and protocols
著者
書誌事項
Cell senescence : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 965）（Springer protocols）
Humana Press, c2013
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Cell senescence is the process whereby cells permanently lose the possibility to proliferate without undergoing cell death, and occurs in a plethora of distinct model organisms. In Cell Senescence: Methods and Protocols, expert researchers in the field detail the methods that are now commonly used to study cell senescence, in model organisms encompassing bacteria, fungi, worms, flies, zebrafish, and mammalian cells. These techniques cover the study of all the morphological, biochemical and functional manifestations of senescence at the cellular level and include protocols for population analyses and high-throughput approaches in suitable model organisms. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
目次
1. Cell Senescence as Both a Dynamic and a Static Phenotype
Andrew RJ Young, Masako Narita, and Masahi Narita
2. Senescence Regulation by mTOR
Vjekoslav Dulic
3. Senescence Regulation by the p53 Protein Family
Yingjuan Qian and Xinbin Chen
4. Markers of Cellular Senescence
Amancio Carnero
5. Biomarkers of Cell Senescence Assessed by Imaging Cytometry
Hong Zhao and Zbigniew Darzynkiewicz
6. Cytofluorometic Assessment of Cell Cycle Progression
Ilio Vitale, Mohamed Jemaa, Lorenzo Galluzzi, Didier Metivier, Maria Castedo and Guido Kroemer
7. Quantification of Cell Cycle-arresting Proteins
Oliver Kepp, Isabelle Martins, Laurie Menger, Mickael Michaud, Sandy Adjemian, Abdul Qader Sukkurwala, Lorenzo Galluzzi, and Guido Kroemer
8. Colorimetric Detection of Senescence-associated Beta Galactosidase (SA-Betagal)
9. Chemiluminescent Detection of Senescence-Associated Beta Galactosidase (SA-Betagal)
10. Detection of the Senescence-associated Secretory Phenotype(SASP)
Francis Rodier
11. Unbiased Characterization of the Senescence-associated Secretome using SILAC-Based Quantitative Proteomics
Juan Carlos Acosta, Ambrosius P. Snijders, and Jesus Gil
12. Detection of Senescence-associated Heterochromatin Foci (SAHF)
Katherine M. Aird and Rugang Zhang
13. Monitoring DNA Damage During Cell Senescence
Glyn Nelson and Thomas von Zglinicki
14. Assessment and Quantification of Telomerase Enzyme Activity
Michelle F. Maritz, Laura A. Richards, Karen L. MacKenzie
15. Methods for the Assessment of Telomere Status
Asako J. Nakamura
16. Detection of Nuclear Envelope Alterations in Senescence
Clea Barcena, Fernando G. Osorio and Jose M.P. Freije
17. Measuring Reactive Oxygen Species in Senescent Cels
Joao F. Passos, Satomi Miwa, and Thomas von Zglinicki
18. Quantification of Protein Carbonylation
Nancy D. Wehr and Rodney L. Levine
19. Assays for the Measurement of Lipid Peroxidation
Ana Cipak Gasparovic, Morana Jaganjac, Branka Mihaljevic, Suzana Borovic Sunjic, and Neven Zarkovic
20. Raman Spectroscopy for the Detection of AGEs/ALEs
J. Renwich Beattie, John J. McGarvey, Alan W. Stitt
21. Monitoring Oncogenic B-RAF-induced Senscence in Melanocytes
Sieu L. Tran and Helen Rizos
22. Methods to Investigate the Role of SIRT1 in Endothelial Senescence
Bo Bai and Yu Wang
23. Monitoring Nutrient Signaling Through the Longevity Protein p66SHC1
Sofia Chiatamone Ranieri and Giovambattista Pani
24. Profiling the Metabolic Signature of Senescence
Florian M. Geier, Silke Fuchs, Gabriel Valbuena, Armand M. Leroi, and Jacob G. Bundy
25. Genome-wide RNAi Screening to Identify Regulators of Oncogene-induced Cellular Senescence
Narendra Wajapeyee, Sara Deibler, and Michael R. Gree
26. An Integrated Approach for Monitoring Cell Senescence
Tatiana V. Pospelova, Zhanna V. Chitikova, Valery A. Pospelov
27. Robust Multiparametric Assessment of Cellular Senescence
Clara Correia-Melo, Diana Jurk and Joao F. Passos
28. Assessing Chronological Aging in Bacteria
Stavros Gonidakis and Valter D. Longo
29. Assessing Organismal Aging in the Filamentous Fungas Podospora Anserina
Heinz D. Osiewacz, Andrea Hamann, Sandra Zintel
30. Assessing Chronological Aging in Saccharomyces cerevisiae
Jia Hu, Min Wei, Mario G. Mirisola, and Valter D. Longo
31. Assessing Aging and Senescent Decline in Caenorhabditis elegans: Cohort Survival Analysis
Eirini Lionaki and Nektarios Tavernarakis
32. High Throughput and Longitudinal Analysis of Aging and Senescent Decline in Caenorhabditis elegans
Eirini Lionaki and Nektarios Tavernarakis
33. Assessing Senescence in Drosophila using Video Tracking
Reza Ardekani, Simon Tavare and John Tower
34. Assessing Vascular Senescence in Zebrafish
Sandra Donnini, Antonio Giachetti, and Marian Ziche
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