Lymphoma : methods and protocols

著者

• Küppers, Ralf

書誌事項

Lymphoma : methods and protocols

edited by Ralf Küppers

（Methods in molecular biology / John M. Walker, series editor, 971）（Springer protocols）

Humana Press, c2013

注記

Includes bibliographical references and index

内容説明・目次

内容説明

Lymphomas are lymphoid malignancies derived from B or T lymphocytes, and their study has been and still is paradigmatic for many aspects of cancer research. Lymphoma: Methods and Protocols presents and discusses key methods that are used in lymphoma research, partly specific for lymphoma research but often adaptable to the study of other cancers. By covering a broad variety of methods used in lymphoma research, this book will be of interest not only for hematologists, hematopathologists, and immunologists but also for scientists interested in other fields of cancer research as well as human genetics. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Versatile and cutting-edge, Lymphoma: Methods and Protocols serves researchers studying human physiology with the ultimate goal of understanding and controlling these often terrible diseases.

目次

1. Origin and Pathogenesis of B Cell Lymphomas Marc Seifert, Rene Scholtysik, and Ralf Kuppers 2. Flow Cytometry for Non-Hodgkin and Classical Hodgkin Lymphoma David Wu, Brent L. Wood, and Jonathan R. Fromm 3. Laser-Based Microdissection of Single Cells from Tissue Sections and PCR Analysis of Rearranged Immunoglobulin Genes from Isolated Normal and Malignant Human B Cells Ralf Kuppers, Markus Schneider, and Martin-Leo Hansmann 4. PCR-Based Analysis of Rearranged Immunoglobulin or T-Cell Receptor Genes by GeneScan Analysis or Heteroduplex Analysis for Clonality Assessment in Lymphoma Diagnostics Elke Boone, Brenda Verhaaf, and Anton W. Langerak, on behalf of the EuroClonality / BIOMED-2 Collaborative Study Group 5. Expression Cloning of Human B Cell Immunoglobulins Hedda Wardemann and Juliane Kofer 6. Studying the Replication History of Human B Lymphocytes by Real-Time Quantitative (RQ)-PCR Menno C. van Zelm, Magdalena A. Berkowska, and Jacques J.M. van Dongen 7. The Detection of Chromosomal Translocations Involving the Immunoglobulin Loci in B-Cell Malignancies Martin J.S. Dyer 8. Stereotyped B Cell Receptors in B Cell Leukemias and Lymphomas Nikos Darzentas and Kostas Stamatopoulos 9. Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies Sebastian Boettcher, Matthias Ritgen, and Michael Kneba 10. MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCR Christiane Pott, Monika Bruggemann, Matthias Ritgen, Vincent H.J. van der Velden, Jacques J.M. van Dongen, and Michael Kneba 11. Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation Miriam Sonnet, Constance Bar, Michael Rehli, Dieter Weichenhan, and Christoph Plass 12. Gene Expression Profile Analysis of Lymphomas Katia Basso and Ulf Klein 13. FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas Maciej Giefing and Reiner Siebert 14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens Vu N. Ngo 15. Studying MicroRNAs in Lymphoma Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg 16. Molecular Methods of Virus Detection in Lymphoma Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer 17. High Throughput RNA Sequencing in B-Cell Lymphomas Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz Martin J.S. Dyer 8. Stereotyped B Cell Receptors in B Cell Leukemias and Lymphomas Nikos Darzentas and Kostas Stamatopoulos 9. Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies Sebastian Boettcher, Matthias Ritgen, and Michael Kneba 10. MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCR Christiane Pott, Monika Bruggemann, Matthias Ritgen, Vincent H.J. van der Velden, Jacques J.M. van Dongen, and Michael Kneba 11. Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation Miriam Sonnet, Constance Bar, Michael Rehli, Dieter Weichenhan, and Christoph Plass 12. Gene Expression Profile Analysis of Lymphomas Katia Basso and Ulf Klein 13. FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas Maciej Giefing and Reiner Siebert 14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens Vu N. Ngo 15. Studying MicroRNAs in Lymphoma Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg 16. Molecular Methods of Virus Detection in Lymphoma Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer 17. High Throughput RNA Sequencing in B-Cell Lymphomas Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz Katia Basso and Ulf Klein 13. FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas Maciej Giefing and Reiner Siebert 14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens Vu N. Ngo 15. Studying MicroRNAs in Lymphoma Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg 16. Molecular Methods of Virus Detection in Lymphoma Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer 17. High Throughput RNA Sequencing in B-Cell Lymphomas Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz Maciej Giefing and Reiner Siebert 14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens Vu N. Ngo 15. Studying MicroRNAs in Lymphoma Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg 16. Molecular Methods of Virus Detection in Lymphoma Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer 17. High Throughput RNA Sequencing in B-Cell Lymphomas Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz Elke Boone, Brenda Verhaaf, and Anton W. Langerak, on behalf of the EuroClonality / BIOMED-2 Collaborative Study Group 5. Expression Cloning of Human B Cell Immunoglobulins Hedda Wardemann and Juliane Kofer 6. Studying the Replication History of Human B Lymphocytes by Real-Time Quantitative (RQ)-PCR Menno C. van Zelm, Magdalena A. Berkowska, and Jacques J.M. van Dongen 7. The Detection of Chromosomal Translocations Involving the Immunoglobulin Loci in B-Cell Malignancies Martin J.S. Dyer 8. Stereotyped B Cell Receptors in B Cell Leukemias and Lymphomas Nikos Darzentas and Kostas Stamatopoulos 9. Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies Sebastian Boettcher, Matthias Ritgen, and Michael Kneba 10. MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCR Christiane Pott, Monika Bruggemann, Matthias Ritgen, Vincent H.J. van der Velden, Jacques J.M. van Dongen, and Michael Kneba 11. Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation Miriam Sonnet, Constance Bar, Michael Rehli, Dieter Weichenhan, and Christoph Plass 12. Gene Expression Profile Analysis of Lymphomas Katia Basso and Ulf Klein 13. FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas Maciej Giefing and Reiner Siebert 14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens Vu N. Ngo 15. Studying MicroRNAs in Lymphoma Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg 16. Molecular Methods of Virus Detection in Lymphoma Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer 17. High Throughput RNA Sequencing in B-Cell Lymphomas Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz Martin J.S. Dyer 8. Stereotyped B Cell Receptors in B Cell Leukemias and Lymphomas Nikos Darzentas and Kostas Stamatopoulos 9. Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies Sebastian Boettcher, Matthias Ritgen, and Michael Kneba 10. MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCR Christiane Pott, Monika Bruggemann, Matthias Ritgen, Vincent H.J. van der Velden, Jacques J.M. van Dongen, and Michael Kneba 11. Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation Miriam Sonnet, Constance Bar, Michael Rehli, Dieter Weichenhan, and Christoph Plass 12. Gene Expression Profile Analysis of Lymphomas Katia Basso and Ulf Klein 13. FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas Maciej Giefing and Reiner Siebert 14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens Vu N. Ngo 15. Studying MicroRNAs in Lymphoma Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg 16. Molecular Methods of Virus Detection in Lymphoma Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer 17. High Throughput RNA Sequencing in B-Cell Lymphomas Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz Katia Basso and Ulf Klein 13. FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas Maciej Giefing and Reiner Siebert 14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens Vu N. Ngo 15. Studying MicroRNAs in Lymphoma Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg 16. Molecular Methods of Virus Detection in Lymphoma Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer 17. High Throughput RNA Sequencing in B-Cell Lymphomas Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz Maciej Giefing and Reiner Siebert 14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens Vu N. Ngo 15. Studying MicroRNAs in Lymphoma Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg 16. Molecular Methods of Virus Detection in Lymphoma Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer 17. High Throughput RNA Sequencing in B-Cell Lymphomas Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz

「Nielsen BookData」 より

詳細情報

• NII書誌ID(NCID): BB26273768
• ISBN:
  • 9781627032681
• LCCN: 2012951649
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York
• ページ数/冊数: xi, 315 p.
• 大きさ: 27 cm
• 分類:
  • LCC : RC280.L9
  • NLM : W1
  • NLM : WH 25
  • DC23 : 616.99/446
  • NDC9 : 493.29
• 件名:
  • LCSH : Lymphomas
  • MESH : Lymphoma -- Laboratory Manuals
  • MESH : B-Lymphocytes -- Laboratory Manuals
  • MESH : Clinical Laboratory Techniques -- methods -- Laboratory Manuals
  • MESH : Immunophenotyping -- methods -- Laboratory Manuals
• 親書誌ID:
  • BA10794673
  • BA88873632