Colorectal cancer : methods and protocols

著者

• Beaulieu, Jean-François

書誌事項

Colorectal cancer : methods and protocols

edited by Jean-François Beaulieu

（Methods in molecular biology / John M. Walker, series editor, 1765）（Springer protocols）

Humana Press, c2018

注記

Includes bibliographical references and index

内容説明・目次

内容説明

This volume explores the latest developments in the study of the mechanisms, diagnostics, screening methods, and therapeutics of colorectal cancer. The book's chapters are divided into three parts: the chapters in Part One examine techniques used to study the molecular mechanisms in colorectal cancer development and progression. Part Two focuses on the innovative tools used to diagnose and detect cancer lesions in the early stages of cancer. Finally, Part Three discusses recent advancements in treating colorectal tumors and identifying new therapeutic molecules for treatment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Colorectal Cancer: Methods and Protocols is a valuable resource for any scientist and researcher interested in this field of study.

目次

Dedication... Preface... Table of Contents... Contributing Authors... Part I Molecular Basis of Colorectal Cancer 1 Cell Line Models of Molecular Subtypes of Colorectal Cancer Jennifer Mooi, Ian Luk, and John Mariadason 2 Dissecting Oncogenic RTK Pathways in Colorectal Cancer Initiation and Progression Stephen McManus, Walid Chababi, Dominique Arsenault, Claire M. Dubois, and Caroline Saucier 3 Identification of Response Elements on Promoters Using Site Directed Mutagenesis and Chromatin Immunoprecipitation Salah Boudjadi, Julie C. Carrier, and Jean-Francois Beaulieu 4 Identification and Functional Analysis of Gene Regulatory Sequences Interacting with Colorectal Tumor Suppressors Katja Dahlgaard and Jesper Thorvald Troelsen 5 Methods for In Vivo Functional Studies of Chromatin-Modifying Enzymes in Early Steps of Colon Carcinogenesis Martine Chevillard-Briet and Fabrice Escaffit 6 The Colorectal Cancer Microenvironment: Strategies for Studying the Role of Cancer Associated Fibroblasts Rahul Bhome, Massimiliano Mellone, Katherine Emo, Gareth Thomas, Emre Sayan, and Alex H. Mirnezami 7 Methods for Assessing Apoptosis and Anoikis in Normal Intestine/Colon and Colorectal Cancer Pierre H. Vachon 8 Molecular Analysis of the Microbiome in Colorectal Cancer Fiona Clegg, Susan H. Berry, Richard Hansen and Georgina L. Hold 9 Proteomic Analysis of Colorectal Cancer Cells Anais Chauvin and Francois-Michel Boisvert 10 Autophagic Flux Assessment in Colorectal Cancer Cells Annie Lauzier and Steve Jean Part II New Developments in Colorectal Cancer Diagnostics and Screening 11 Classification of Colorectal Cancer in Molecular Subtypes by Immunohistochemistry Sanne ten Hoorn, Anne Trinh, Joan de Jong, Lianne Koens, and Louis Vermeulen 12 Stool DNA Integrity Method for Colorectal Cancer Detection Claudia Rengucci, Giulia De Maio, Maura Menghi, and Daniele Calistri 13 RT-qPCR for Fecal Mature microRNA Quantification and Validation Farid E. Ahmed, Nancy C. Ahmed, Mostafa M. Gouda, Paul W. Vos, and Chris Bonnerup 14 A Stool Multi-Target mRNA Assay for the Detection of Colorectal Neoplasms Elizabeth Herring, Shigeru Kanaoka, Eric Tremblay, and Jean-Francois Beaulieu 15 Colorectal Cancer Detection Using Targeted LC-MS Metabolic Profiling Danijel Djukovic, Jiangjiang Zhang, and Daniel Raftery 16 Proteomic Profiling for Colorectal Cancer Biomarker Discovery Paula Alvarez-Chaver, Loretta De Chiara, and Vicenta Soledad Martinez-Zorzano 17 Tumor-Derived Microparticles to Monitor Colorectal Cancer Evolution Diane Mege, Laurence Panicot-Dubois, and Christophe Dubois Part III Recent Advances in Colorectal Cancer Therapeutics 18 Molecular Testing for the Treatment of Advanced Colorectal Cancer: An Overview Patrick S. Lin and Thomas J. Semrad 19 Testing Cell-Based Immunotherapy for Colorectal Cancer Jong Soon Kang, Yeo Dae Yoon, Myeong Youl Lee, Chang Woo Lee, Suk Jong Lee, and Sang-Bae Han 20 Patient-Derived Xenograft Models of Colorectal Cancer: Procedures for Engraftment and Propagation Danielle M. Burgenske, David J. Monsma, and Jeffrey P. MacKeigan 21 Use of Organoids to Characterize Signaling Pathways in Cancer Initiation Christina Oatway, Calley L. Hirsch, and Alex Gregorieff 22 Identification of Novel Molecules Targeting Cancer Stem Cells Yannick D. Benoit

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詳細情報

• NII書誌ID(NCID): BB26660072
• ISBN:
  • 9781493977642
• LCCN: 2018936904
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York
• ページ数/冊数: xiv, 353 p.
• 大きさ: 27 cm
• 親書誌ID:
  • BA10794673
  • BA88873632