Photosynthesis : methods and protocols
Author(s)
Bibliographic Information
Photosynthesis : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1770）（Springer protocols）
Humana Press, c2018
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Note
Includes bibliographical references and index
Description and Table of Contents
Description
This user-friendly book provides a range of classical and modern techniques for the study of photosynthesis in a manner accessible to a broad spectrum of researchers. Broken into four sections, it explores the measurement of physiological photosynthetic parameters, quantifying photosynthetic enzyme abundance and catalytic activity, visualizing cellular and sub-cellular phenotypes, and photosynthesis-inspired energy generation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Photosynthesis: Methods and Protocols aims to aid scientists working toward exciting developments in the understanding and application of photosynthesis.
Table of Contents
Part I: Measuring Photosynthetic Parameters
1. Survey of Tools for Measuring In Vivo Photosynthesis
Berkley J. Walker, Florian A. Busch, Steven M. Driever, Johannes Kromdijk, and Tracy Lawson
2. Photosynthetic Gas Exchange in Land Plants at the Leaf Level
Florian A. Busch
3. Design and Use of a Digitally-Controlled Device for Accurate, Multiplexed Gas Exchange Measurements of the Complete Foliar Parts of Plants
Gavin M. George, Katharina Koelling, Roland Kuenzli, Matthias Hirsch-Hoffmann, Patrick Flutsch, and Samuel C. Zeeman
4. Measuring Canopy Gas Exchange Using CAnopy Photosynthesis and Transpiration Systems (CAPTS)
Qingfeng Song and Xin-Guang Zhu
5. Light-Response Curves in Land Plants
Robert A. Coe and HsiangChun Lin
6. Chlorophyll Fluorescence on the Fast Timescale
Olubukola O. Ajigboye, Rumiana V. Ray, and Erik H. Murchie
7. Sub-Saturating Multiphase Flash Irradiances to Estimate Maximum Fluorescence Yield
Thomas J. Avenson and Aaron J. Saathoff
8. Chlorophyll Fluorescence Imaging
Tracy Lawson and Silvere Vialet-Chabrand
9. Measurement of O2 Uptake and Evolution in Leaves In Vivo Using Stable Isotopes and Membrane Inlet Mass Spectrometry
Steven M. Driever and Neil R. Baker
10. Using Carbon Stable Isotopes to Study C3 and C4 Photosynthesis: Models and Calculations
Nerea Ubierna, Meisha-Marika Holloway-Phillips, and Graham D. Farquhar
11. Liquid-Phase Measurements of Photosynthetic Oxygen Evolution
Dmitriy Shevela, Wolfgang P. Schroeder, and Johannes Messinger
Part II: Measuring Photosynthetic Enzyme Abundance and Catalytic Activity
12. Quantification of Photosynthetic Enzymes in Leaf Extracts by Immunoblotting
J. Alejandro Perdomo, Cristina R.G. Sales, and Elizabete Carmo-Silva
13. Extraction of Rubisco to Determine Catalytic Constants
Douglas J. Orr and Elizabete Carmo-Silva
14. Spectrophotometric Determination of Rubisco Activity and Activation State in Leaf Extracts
Cristina R.G. Sales, Gustaf E. Degen, Anabela Bernardes da Silva, and Elizabete Carmo-Silva
Part III: Determining Cellular and Sub-Cellular Phenotypes
15. Creating Leaf Cell Suspensions for Characterization of Mesophyll and Bundle Sheath Cellular Features
Roxana Khoshravesh and Tammy L. Sage
16. Determining the Subcellular Localization of Fluorescently-Tagged Proteins Using Protoplasts Extracted from Transiently-Transformed Nicotiana benthamiana Leaves
Vivien Rolland
17. 3D Clearing and Molecular Labeling in Plant Tissues
William M. Palmer, Jamie R. Flynn, Antony P. Martin, Stephanie L. Reed, Christopher P.L. Grof, Rosemary G. White, and Robert T. Furbank
18. Evaluation of Lipids for the Study of Photosynthetic Membranes
Helmut Kirchhoff and Robert Yarbrough
Part IV: Photosynthesis-Inspired Energy Generation
19. "Click" Methodology for the Functionalization of Water Oxidation Catalyst Iridium Oxide Nanoparticles with Hydrophobic Dyes for Artificial Photosynthetic Constructs
Jackson D. Megiatto Jr. and Catia Ornelas
20. Biophotovoltaics: Design and Study of Bio-Electrochemical Systems for Biotechnological Applications and Metabolic Investigation
Stephen J.L. Rowden, Paolo Bombelli, and Christopher J. Howe
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