Plant metabolomics : methods and protocols

著者

    • António, Carla

書誌事項

Plant metabolomics : methods and protocols

edited by Carla António

（Methods in molecular biology / John M. Walker, series editor, 1778）（Springer protocols）

Humana Press, c2018

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

This detailed book aims to address the need for standardizing key steps in plant metabolomics research that is still considered a critical issue in the field. The collection unites diverse mass spectrometry (MS)-based protocols, from sample preparation and extraction of plant primary and secondary metabolites (e.g. a range of sugars, lipids, phytohormones and volatile organic compounds) to metabolite analysis, using exclusively highly sensitive MS-based methodologies, followed by frontline bioinformatics and/or mathematical modeling approaches to produce reliable biological data interpretation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Metabolomics: Methods and Protocols serves both new and well-established researchers in the plant metabolomics field in finding helpful guidelines and useful laboratory-based protocols for setting up their routine plant metabolomics experiments.

目次

Part I: Review Chapters 1. The Importance of Experimental Design, Quality Assurance, and Control in Plant Metabolomics Experiments Marina C.M. Martins, Camila Caldana, Lucia Daniela Wolf, and Luis Guilherme Furlan de Abreu 2. Standard Key Steps in Mass Spectrometry-Based Plant Metabolomics Experiments: Instrument Performance and Analytical Method Validation Ana Margarida Rodrigues and Carla Antonio 3. Guidelines for Sample Normalization to Minimize Batch Variation for Large-Scale Metabolic Profiling of Plant Natural Genetic Variance Saleh Alseekh, Si Wu, Yariv Brotman, and Alisdair R. Fernie 4. Gas Chromatography-Mass Spectrometry-Based 13C-Labeling Studies in Plant Metabolomics Valeria F. Lima, Leonardo P. Souza, Thomas C.R. Williams, Alisdair R. Fernie, and Danilo M. Daloso 5. Carbon Atomic Survey for Identification of Selected Metabolic Fluxes Leonardo Perez de Souza, Alisdair R. Fernie, and Takayuki Tohge Part II: Mass Spectrometry-Based Methodology Chapters 6. Quantification of Low-Abundant Phosphorylated Carbohydrates Using HILIC-QqQ-MS/MS Tiago F. Jorge and Carla Antonio 7. Quantification of Soluble Sugars and Sugar Alcohols by LC-MS/MS Regina Feil and John Edward Lunn 8. Untargeted Analysis of Semi-Polar Compounds by LC-MS and Targeted Analysis of Fatty Acids by GC-MS/GC-FID: From Plant Cultivation to Extract Preparation Camille Benard, Sebastien Acket, Yannick Rossez, Olivier Fernandez, Thierry Berton, Yves Gibon, and Cecile Cabasson 9. Extraction of Plant Lipids for LC-MS-Based Untargeted Plant Lipidomics Thusitha W.T. Rupasinghe and Ute Roessner 10. Semi-Targeted Lipidomics of Plant Acyl Lipids Using UPLC-HR-MS in Combination with a Data-Independent Acquisition Mode Mohamed A. Salem and Patrick Giavalisco 11. Plant Lipidomics Using UPLC-QTOF-MS Yozo Okazaki and Kazuki Saito 12. Targeted LC-MS Analysis for Plant Secondary Metabolites Takafumi Shimizu, Mutsumi Watanabe, Alisdair R. Fernie, and Takayuki Tohge 13. An UHPLC-MS/MS Method for Target Profiling of Stress-Related Phytohormones Ondrej Novak and Kristyna Flokova 14. A Complete Pipeline for Generating a High-Resolution LC-MS-Based Reference Mass Spectra Library Nir Shahaf, Asaph Aharoni, and Ilana Rogachev 15. Quantification of Sugars and Organic Acids in Biological Matrices Using GC-QqQ-MS Nirupama Samanmalie Jayasinghe, Himasha Mendis, Ute Roessner, and Daniel Anthony Dias 16. Acquisition of Volatile Compounds by Gas Chromatography-Mass Spectrometry (GC-MS) Jose G. Vallarino, Alexander Erban, Ines Fehrle, Alisdair R. Fernie, Joachim Kopka, and Sonia Osorio 17. Mass Spectrometry Imaging (MSI) for Plant Metabolomics Berin A. Boughton and Dinaiz Thinagaran 18. Laser Ablation Electrospray Ionization-Mass Spectrometry Imaging (LAESI-MS) for Spatially Resolved Plant Metabolomics Desalegn Etalo, Carmen Diez-Simon, Ric C.H. de Vos, and Robert D. Hall 19. Live Single-Cell Mass Spectrometry (LSC-MS) for Plant Metabolomics Keiko Masuda, Yasmine Abouleila, Ahmed Ali, Toshio Yanagida, and Tsutomu Masujima Part III: Data Analysis & Statistics Chapters 20. Statistical and Multivariate Analysis of MS-Based Plant Metabolomics Data Carsten Jaeger and Jan Lisec 21. Integration of Plant Metabolomics Data into Metabolic Networks: Progresses and Challenges Nadine Toepfer, Samuel M.D. Seaver, and Asaph Aharoni 22. Understanding the Functionality of a Biological System as a Whole: Comparative Data Analysis Isabel Orf 23. Regression-Based Modeling of Complex Plant Traits Based on Metabolomics Data Francisco de Abreu e Lima, Lydia Leifels, and Zoran Nikoloski 24. Mathematical Modeling Approaches in Plant Metabolomics Lisa Furtauer, Jakob Weiszmann, Wolfram Weckwerth, and Thomas Nagele

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