Plastids : methods and protocols

著者

• Maréchal, Eric

書誌事項

Plastids : methods and protocols

edited by Eric Maréchal

（Methods in molecular biology / John M. Walker, series editor, 1829）（Springer protocols）

Humana Press, c2018

注記

Includes bibliographical references and index

内容説明・目次

内容説明

This volume explores plastid evolution, structure, and function in algae, plants and protists. The methods described in this book help scientists visualize, fractionate, purify, and study primary and secondary plastids in plant and algal materials. The chapters in this book also look at various techniques to analyze plastids through means of combining biology strategies from genetics, genomics, proteomics, and lipidomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Plastids: Methods and Protocols is a valuable resource for students, engineers, and researchers who are interested in this evolving organelle and overall field.

目次

Preface... Table of Contents... Contributing Authors... Part I Plastid Evolution, Plasticity, Structural, and Functional Diversity 1. Primary Endosymbioses: Emergence of the Primary Chloroplast and the Chromatophore, Two Independent Events Eric Marechal 2. Complex Endosymbioses I: From Primary to Complex Plastids, Multiple Independent Events Zoltan Fussy and Miroslav Obornik 3. Complex Endosymbiosis II: The Non-Photosynthetic Plastid of Apicomplexa Parasites (The Apicoplast) and Its Integrated Metabolism Cyrille Y. Botte and Yoshiki Yamaryo- Botte 4. Diversity and Plasticity of Plastids in Land Plants Katalin Solymosi, Johanna Lethin, and Henrik Aronsson 5. The Main Functions of Plastids Norbert Rolland, Imen Bouchnak, Lucas Moyet, Daniel Sali, and Marcel Kuntz 6. Plastid Transient and Stable Interactions with Other Cell Compartments Stefanie J. Mueller-Schuessele and Morane Michaud Part II Laboratory Protocols 7. Imaging Plastids in 2D and 3D: Confocal and Electron Microscopy Serena Flori, Pierre-Henri Jouneau, Benoit Gallet, Leandro F. Estrozi, Christine Moriscot, Guy Schoehn, Giovanni Finazzi, and Denis Falconet 8. Purification of Chloroplasts and Chloroplast Subfractions: Envelope, Thylakoids, and Stroma - From Spinach, Pea, and Arabidopsis thaliana Maryse A. Block and Catherine Albrieux 9. Isolation of Inner and Outer Membranes of the Chloroplast Envelope from Spinach and Pea Maryse A. Block 10. Purification of Non-Green Plastids (Proplastids and Amyloplasts) from Angiosperms, and Isolation of Their Envelope Membranes Claude Alban and Etienne-Pascal Journet 11. In Vitro Protein Import into Isolated Chloroplasts Maryse A. Block 12. Analysis of the MTL Super-Complex at Contact Sites between Mitochondria and Plastids Morgane Michaud 13. Isolation of Plastid-Fractions from the Diatoms Thalassiosira pseudonana and Phaeodactylum tricornutum Alexander Schober, Serena Flori, Giovanni Finazzi, Peter G. Kroth, and Carolina Rio Bartulos 14. Isolating the Plasmodium falciparum Apicoplast using Magnetic Beads Cyrille Y. Botte, Geoffrey I. McFadden, and Yoshiki Yamaryo-Botte 15. Extraction and Quantification of Lipids from Plant or Algae Valerie Gros, Josselin Lupette, and Juliette Jouhet 16. Quantitative Assessment of the Chloroplast Lipidome Valerie Gros and Juliette Jouhet 17. Determination of the DNA/RNA-Associated Sub-Proteome from Chloroplasts and Other Plastid Types Maha Chieb, Monique Liebers, Fabien Chevalier, Silva Lerbs-Mache, Robert Blanvillain, and Thomas Pfannschmidt 18. Mapping Plastid Transcript Population by Circular Reverse Transcription-Polymerase Chain Reaction Florence Courtois and Livia Merendino 19. Bioinformatic Analysis of Chloroplast Gene Expression and RNA Post-Transcriptional Maturations using RNA Sequencing Bastien Malbert, Guillen Rigaill, Veronique Brunaud, Claire Lurin, and Etienne Delannoy 20. A Guide to the Chloroplast Transcriptome Analysis using RNA-Seq Elena J.S. Michel, Amber M. Hotto, Susan R. Strickler, David B. Stern, and Benoit Castandet 21. A Toolkit for the Characterization of the Photoprotective Capacity of Green Algae Aguila Ruiz-Sola and Dimitris Petroutsos 22. Rescue of Deletion Mutants to Isolate Plastid Transformants in Higher Plants Mohammad El Hajj, Mohd Fahdli Bin Hamdan, Elena Martin Avila, and Anil Day 23. Genetic Analysis of Chloroplast Biogenesis, and Function and Mutant Collections Sho Fujii, Hajime Wada, and Koichi Kobayashi 24. Generation of Mutants of Nuclear-Encoded Plastid Proteins Using CRISPR/Cas9 in the Diatom Phaeodactylum tricornutum Guillaume Allorent, Erika Guglielmino, Cecile Giustini, and Florence Courtois Part III In Silico Tools 25. In Silico Tools for the Prediction of Protein Import into Secondary Plastids Daniel Moog 26. AT_CHLORO - The First Step When Looking for Information about Subplastidial Localization of Proteins Daniel Salvi, Sylvain Bournais, Lucas Moyet, Imen Bouchnak, Marcel Kuntz, Christophe Bruley, and Norbert Rolland

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詳細情報

• NII書誌ID(NCID): BB26746156
• ISBN:
  • 9781493986538
• LCCN: 2018947772
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York
• ページ数/冊数: xv, 409 p.
• 大きさ: 27 cm
• 親書誌ID:
  • BA10794673
  • BA88873632