Viral vectors for gene therapy : methods and protocols

著者

書誌事項

Viral vectors for gene therapy : methods and protocols

edited by Otto-Wilhelm Merten, Mohamed Al-Rubeai

（Methods in molecular medicine / John M. Walker, series editor, 737）

Humana Press, c2011

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

The huge potential for gene therapy to cure a wide range of diseases has led to high expectations and a great increase in research efforts in this area, particularly in the study of delivery via viral vectors, widely considered to be more efficient than DNA transfection. In Viral Vectors for Gene Therapy: Methods and Protocols, experts in the field present a collection of their knowledge and experience featuring methodologies that involve virus production, transferring protocols, and evaluating the efficacy of gene products. While thoroughly covering the most popular viral vector systems of adenovirus, retrovirus, and adeno-associated virus, this detailed volume also explores less common viral vector systems such as baculovirus, herpes virus, and measles virus, the growing interest in which is creating a considerable demand for large scale manufacturing and purification procedures. Written in the highly successful Methods in Molecular Biology (TM) series format, many chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Viral Vectors for Gene Therapy: Methods and Protocols provides basic principles accessible to scientists from a wide variety of backgrounds for the development of gene therapy viral products that are safe and effective.

目次

1. Introduction to Viral Vectors James N. Warnock, Claire Daigre, and Mohamed Al-Rubeai 2. Introduction to Gene Therapy: A Clinical Aftermath Patrice P. Denefle 3. Host Cells and Cell Banking Glyn N. Stacey and Otto-Wilhelm Merten 4. Overview of Current Scalable Methods for Purification of Viral Vectors Maria Mercedes Segura, Amine Kamen, and Alain Garnier 5. Methods to Construct Recombinant Adenovirus Vectors Miguel Chillon and Ramon Alemany 6. Manufacturing of Adenoviruses Vectors: Production and Purification of Helper Dependent Adenovirus Edwige Dormond and Amine A. Kamen 7. Manufacturing of Retroviruses Pedro E. Cruz, Teresa Rodrigues, Marlene Carmo, Dagmar Wirth, Ana Amaral, Paula M. Alves, and Ana S. Coroadinha 8. Lentiviral Vectors Marc Giry-Laterriere, Els Verhoeyen, and Patrick Salmon 9. Adeno-Associated Viruses Mauro Mezzina and Otto-Wilhelm Merten 10. Manufacturing of Adeno-Associated Viruses, for Example: AAV2 Haifeng Chen 11. Vector Characterization Methods for Quality Control Testing of Recombinant Adeno-Associated Viruses J. Fraser Wright and Olga Zelenaia 12. Baculoviruses Mediate Efficient Gene Expression in a Wide Range of Vertebrate Cells Kari J. Airenne, Kaisa-Emilia Makkonen, Anssi J. Mahoenen, and Seppo Yla-Herttuala 13. Herpes Simplex Virus Type 1 (HSV-1)-Derived Recombinant and Amplicon Vectors Cornel Fraefel, Peggy Marconi, and Alberto L. Epstein 14. Manufacture of Measles Viruses Kirsten K. Langfield, Henry J. Walker, Linda C. Gregory, and Mark J. Federspiel 15. In Vivo Gene Delivery into hCD34+ Cells in a Humanized Mouse Model Cecilia Frecha, Floriane Fusil, Francois-Loic Cosset, and Els Verhoeyen 16. In Vivo Evaluation of Gene Transfer into Mesenchymal Cells (in View of Cartilage Repair) Kolja Gelse and Holm Schneider 17. Ethical Consideration Michael Fuchs 18. Clinical Trials of GMP Products in the Gene Therapy Field Kathleen B. Bamford

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