The Golgi complex : methods and protocols

著者

    • Brown, William J. (Professor of cell biology)

書誌事項

The Golgi complex : methods and protocols

edited by William J. Brown

（Methods in molecular biology, 1496）（Springer protocols）

Humana Press : Springer Science+Business Media, c2016

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

Focusing on new methods and techniques developed to address longstanding questions about the Golgi complex, this volume explores a diverse set of chapters, ranging from live and fixed cell imaging techniques to in vitro biochemical reconstitution systems. Each chapter provides a detailed set of specific instructions, which should enable anyone to successfully complete the assays. Written for the highly successful Methods in Molecular Biology series program, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips from the experts on troubleshooting and avoiding known pitfalls. Practical and detailed, The Golgi Complex: Methods and Protocols will aid both new and established researchers in the field by providing strong hands-on instructions that can be directly applied to their research programs.

目次

1. 4D Confocal Imaging of Yeast Organelles Kasey J. Day, Effrosyni Papanikou, and Benjamin S. Glick 2. Imaging the Polarized Sorting of Proteins from the Golgi Complex in Live Neurons Ginny G. Farias, Dylan J. Britt, and Juan S. Bonifacino 3. Imaging Golgi Outposts in Fixed and Living Neurons Mariano Bisbal, Gonzalo Quassollo, and Alfredo Caceres 4. Analysis of Arf1 GTPase-Dependent Membrane Binding and Remodeling Using the Exomer Secretory Vesicle Cargo Adaptor Jon E. Paczkowski and J. Christopher Fromme 5. STEM Tomography Imaging of Hypertrophied Golgi Stacks in Mucilage-Secreting Cells Byung-Ho Kang 6. Reconstitution of COPI Vesicle and Tubule Formation Seung-Yeol Park, Jia-Shu Yang, and Victor W. Hsu 7. Reconstitution of Phospholipase A2-Dependent Golgi Membrane Tubules Edward B. Cluett, Paul de Figueiredo, Marie E. Bechler, Kevin D. Thorsen, and William J. Brown 8. Proteomic Characterization of Golgi Membranes Enriched from Arabidopsis Suspension Cell Cultures Sara Fasmer Hansen, Berit Ebert, Carsten Rautengarten, and Joshua L. Heazlewood 9. High Content Analysis of the Golgi Complex by Correlative Screening Microscopy Manuel Gunkel, Holger Erfle, and Vytaute Starkuviene 10. Activity Detection of GalNAc Transferases by Protein-Based Fluorescence Sensors In Vivo Lina Song, Collin Bachert, and Adam D. Linstedt 11. In Situ Proximity Ligation Assay (PLA) Analysis of Protein Complexes Formed Between Golgi-Resident, Glycosylation-Related Transporters and Transferases in Adherent Mammalian Cell Cultures Dorota Maszczak-Seneczko, Paulina Sosicka, Teresa Olczak, and Mariusz Olczak 12. Creating Knock-Outs of Conserved Oligomeric Golgi Complex Subunits Using CRISPR-Mediated Gene Editing Paired with a Selection Strategy Based on Glycosylation Defects Associated with Impaired COG Complex Function Jessica Bailey Blackburn and Vladimir V. Lupashin 13. Reversible Controlled Aggregation of Golgi Resident Enzymes to Assess Their Transport/Dynamics Along the Secretory Pathway Riccardo Rizzo and Alberto Luini 14. Assays to Study the Fragmentation of the Golgi Complex During the G2/M Transition of the Cell Cycle Inmaculada Ayala and Antonino Colanzi 15. The Role of Lysophospholipid Acyltransferases in the Golgi Complex John A. Schmidt 16. Methods to Purify and Assay Secretory Pathway Kinases Vincent S. Tagliabracci, Jianzhong Wen, and Junyu Xiao

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