Animal coronaviruses

著者

    • Wang, Leyi

書誌事項

Animal coronaviruses

edited by Leyi Wang

（Springer protocols handbooks）

Humana Press, c2016

大学図書館所蔵 件 / 全2件

この図書・雑誌をさがす

注記

Includes bibliographical references and index

内容説明・目次

内容説明

This detailed volume provides diagnosticians and researchers with practical methodologies and approaches to tackle animal coronaviruses. It explores conventional immunohistochemistry, virus neutralization, enzyme-linked immunosorbent assays, expression and purification of recombinant viral proteins, and various molecular assays, including conventional and real-time reverse transcription-PCR, reverse genetics methodology, and next generation sequencing and sequence analyses. As part of the Springer Protocols Handbooks series, chapters contain readily reproducible laboratory protocols as well as expert tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Animal Coronaviruses serves as an ideal reference for researchers examining a wide variety of coronavirus species in the Coronaviridae.

目次

Part I: Introduction 1. Animal Coronaviruses: A Brief Introduction Leyi Wang and Yan Zhang Part II: Conventional Technologies 2. Immunohistochemical Staining for Detection of Porcine Epidemic Diarrhea Virus in Tissues Kwonil Jung 3. Virus Neutralization Assay for Turkey Coronavirus Infection Yi-Ning Chen, Ching Ching Wu, and Tsang Long Lin 4. Recombinant Turkey Coronavirus Nucleocapsid Protein Expressed in Escherichia coli Chien Chang Loa, Ching Ching Wu, and Tsang Long Lin 5. Antibody-Capture Enzyme-Linked Im munosorbent Assay for Detection of Antibody to Turkey Coronavirus Using Infectious Bronchitis Virus or Recombinant Nucleocapsid Protein as Coating Antigen Chien Chang Loa, Mohamed Abdelwahab, Yi-Ning Chen, Ming-Kun Hsieh, Ching Ching Wu, and Tsang Long Lin Part III: Reverse Genetics 6. Reverse Genetics of Avian Coronavirus Infectious Bronchitis Virus Sarah M. Keep, Erica Bickerton, and Paul Britton 7. Vaccinia Virus-Based Reverse Genetics for Feline Coronaviruses Gergely Tekes Part IV: Molecular Diagnostics 8. Real-Time RT-PCR Detection of Equine Coronavirus Fabien Miszczak, Nathalie Kin, Vincent Tesson, and Astrid Vabret 9. Detection of Bovine Coronavirus by Conventional Reverse Transcription Polymerase Chain Reaction Amauri Alcindo Alfieri, Alice Fernandes Alfieri, and Elisabete Takiuchi 10. Real-Time Reverse Transcription Polymerase Chain Reaction for Rapid Detection of Transmissible Gastroenteritis Virus Ramesh Vemulapalli 11. An RT-PCR Assay for Detection of Infectious Bronchitis Coronavirus Serotypes Junfeng Sun and Shengwang Liu 12. A Multiplex Polymerase Chain Reaction for Differential Detection of Turkey Coronavirus from Chicken Infectious Bronchitis Virus and Bovine Coronavirus Chien Chang Loa, Ching Ching Wu, and Tsang Long Lin 13. Real-Time Reverse Transcription-polymerase Chain Reaction for Detection and Quantitation of Turkey Coronavirus RNA in Feces and Intestine Tissues Yi-Ning Chen, Ching Ching Wu, and Tsang Long Lin 14. PCR Amplification and Sequencing Analysis of Full-Length Turkey Coronavirus Spike Gene Yi-Ning Chen, Aydemir Akin, Chien Chang Loa, Mustafa Ababneh, Jianzhong Cao, Wan-Jung Chen, Ching Ching Wu, and Tsang Long Lin 15. Feline Coronavirus RT-PCR Assays for Feline Infectious Peritonitis Diagnosis Takehisa Soma 16. Genotype-Specific Detection of Ferret Coronavirus by Conventional and Real-Time Reverse Transcription Polymerase Chain Reaction Annabel G. Wise, Matti Kiupel, and Roger K. Maes 17. Molecular Charac terization of Canine Coronavirus Rita de Cassia Nasser Cubel Garcia Part V: Next Generation Sequencing 18. Whole Genome Sequencing of Porcine Epidemic Diarrhea Virus by Illumina MiSeq Platform Leyi Wang, Tod Stuber, Patrick Camp, Suelee Robbe-Austerman, and Yan Zhang 19. Next Generation Sequencing for Porcine Coronaviruses Douglas Marthaler, Ann Bohac, Aaron Becker, and Nichole Peterson

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