Mouse embryogenesis : methods and protocols
Author(s)
Bibliographic Information
Mouse embryogenesis : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1752）（Springer protocols）
Humana Press, c2018
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Note
Includes biblographical references and index
Description and Table of Contents
Description
This volume explores protocols for identifying mutant mice and characterizing parts of their anatomical, functional, cellular, and molecular phenotypes. The chapters in this book look at anatomical and functional phenotyping using quantitative imaging, isolation of specific embryonic cell types for cell culture, analysis of gene expression, and how to define chromatin structure. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Mouse Embryogenesis: Methods and Protocols is a valuable resource for experimentalists interested in discovering new aspects of embryogenesis control, organ function, and the origin of disease.
Table of Contents
Preface...
Table of Contents...
Contributing Authors...
1. Mouse Genotyping
Sandra Vuong and Paul Delgado-Olguin
2. Visualizing the Vascular Network in the Mouse Embryo and Yolk Sac
Anna R. Roy and Paul Delgado-Olguin
3. In Vivo Evaluation of the Cardiovascular System of Mouse Embryo and Fetus Using High Frequency Ultrasound
Yu-Qing Zhou, Lindsay S. Cahill, and John G. Sled
4. Dynamic Imaging of Mouse Embryos and Cardiodynamics in Static Culture
Andrew L. Lopez, III and Irina V. Larina
5. In Vivo Imaging of the Mouse Reproductive Organs, Embryo Transfer, and Oviduct Cilia Dynamics Using Optical Coherence Tomography
Shang Wang and Irina V. Larina
6. Live Imaging of Fetal Intra-Abdominal Organs Using Two-Photon Laser-Scanning Microscopy
Yuhki Koike, Bo Li, Yong Chen, Hiromu Miyake, Carol Lee, Lijun Chi, Richard Wu, Mikihiro Inoue, Keiichi Uchida, Masato Kusunoki, Paul Delgado Olguin, and Agostino Pierro
7. Embrionary Mouse Cardiac Fibroblast Isolation
Alejandra Garate-Carrillo and Israel Ramirez-Sanchez
8. Explant Culture for Studying Lung Development
Behzad Yeganeh, Claudia Bilodeau, and Martin Post
9. Isolating Embryonic Cardiac Progenitors and Cardiac Myocytes by Fluorescence-Activated Cell Sorting
Abdalla Ahmed and Paul Delgado-Olguin
10. Isolation and Culture of Mouse Placental Endothelial Cells
Lijun Chi and Paul Delgado-Olguin
11. Flow Cytometry and Lineage Tracing Study for Identification of Adipocyte Precursor Cell (APCs) Populations
Ju Hee Lee, Azadeh Yeganeh, Hisato Konoeda, Joon Ho Moon, and Hoon-Ki Sung
12. Whole-Mount and Section In Situ Hybridization in Mouse Embryos for Detecting mRNA Expression and Localization
Kazuko Koshiba-Takeuchi
13. Chromosome Painting of Mouse Chromosomes
Lisa L. Hua and Takashi Mikawa
14. Chromatin Immunoprecipitation in Early Mouse Embryos
Estela Garcia-Gonzalez, Bladimir Roque-Ramirez, Carlos Palma-Flores, and J. Manuel
Hernandez-Hernandez
15. Shaping Up the Embryo: The Role of Genome 3D Organization
Karina Jacome-Lopez and Mayra Furlan-Magaril
16. Genome Edition during Development Using the CRISPR-Cas Technology
Rodrigo Arzate-Mejia, Paula Licona-Limon, and Felix Recillas-Targa
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