Histone Variants : methods and Protocols

著者

• Orsi, Guillermo A.
• Almouzni, Geneviève

書誌事項

Histone Variants : methods and Protocols

edited by Guillermo A. Orsi, Geneviève Almouzni

（Springer protocols）（Methods in molecular biology / John M. Walker, series editor, 1832）

Humana Press/Springer, c2018

注記

Includes bibliographical references and index

内容説明・目次

内容説明

This volume compiles methodologies used for detailed studies of histone variants, from their basic properties to their functional roles in chromatin and as vectors of epigenetic information. Its four sections cover experimental approaches to probe the biochemistry of histone variants and variant nucleosomes; their dynamics throughout the cell cycle and at specific genomic locations; their functional impact on chromosome organization and genome stability; and their importance in development, disease and evolution. Written in the highly successful Methods in Molecular Biology series format, chapters include lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, illustrations on the expected results, and tips on troubleshooting and avoiding known pitfalls. This anthology brings together contributions from many of the most highly recognized investigators in this community. Cutting-edge and thorough, Histone Variants: Methods and Protocols is a valuable resource for anyone interested in exploring the experimental and collaborative possibilities in this evolving field.

目次

Preface... Table of Contents... Contributing Authors... Part I Biochemistry 1. Methods for Preparing Nucleosomes Containing Histone Variants Tomoya Kujirai, Yasuhiro Arimura, Risa Fujita, Naoki Horikoshi, Shinichi Machida, and Hitoshi Kurumizaka 2. Characterization of Post-Translational Modifications on Histone Variants Francisco Saavedra, Sebastian Marty-Lombardi, and Alejandra Loyola 3. Purification of Histone Variant-Interacting Chaperone Complexes Hideaki Tagami 4. Detection of Histone Modification Dynamics during the Cell Cycle by MS-Based Proteomics Moritz Carl Voelker-Albert, Andreas Schmidt, Teresa Barth, Ignasi Forne, and Axel Imhof Part II Epigenomics 5. Histone Native Chromatin Immunprecipitation Alicia Alonso, Emily Bernstein, and Dan Hasson 6. How to Tackle Challenging ChIP-seq, with Long-Range Cross-Linking, using ATRX as an Example Julia Truch, Jelena Telenius, Douglas R. Higgs, and Richard J. Gibbons 7. Time-ChIP : A Method to Determine Long-Term Locus-Specific Nucleosome Inheritance Wojciech Siwek, Mariluz Gomez-Rodriguez, Daniel Sobral, Ivan R. Correa, and Lars E.T. Jansen 8. MINCE-seq : Mapping In Vivo Nascent Chromatin with EdU and Sequencing Srinivas Ramachandran and Steven Henikoff 9. RChIP-seq : Chromatin-Associated RNA Sequencing in Developmentally Staged Mouse Testes Tatiana A. Soboleva and David J. Tremethick 10. Bioinformatics Analysis of Nucleosome and Histone Variant Positioning Jakub Mieczkowski and Michael Y. Tolstorukov Part III Microscopy and Cell Biology 11. Imaging newly Synthesized and Old Histone Variant Dynamics Dependent on Chaperones Using the SNAP-Tag System Julia Torne, Guillermo Orsi, Dominique Ray-Gallet, and Genevieve Almouzni 12. Real Time de novo Deposition of Centromeric Histone-Associated Proteins Using the Auxin Inducible Degradation System Sebastian Hoffmann and Daniele Fachinetti 13. Live Imaging of Parental Histone Variant Dynamics in UVC-Damaged Chromatin Juliette Dabin, Anna Fortuny, Sandra Piquet, and Sophie E. Polo 14. CRISPR/Cas9 Gene Editing of Human Histone H2A Variant H2AX and MacroH2A Justin W.C. Leung, Lara E. Emery, and Kyle M. Miller Part IV Evolution, Development, and Diseases 15. Studying the Evolution of Histone Variants Using Phylogeny Antoine Molaro and Ines A. Drinnenberg 16. Characterization of Post-Meiotic Male Germ Cell Genome Organizational States Jerome Govin, Sophie Barral, Yuichi Morozumi, Naghmeh Hoghoughi, Thierry Buchou, Sophie Rousseaux, and Saadi Khochbin 17. An Animal Model for Genetic Analysis of Multi-Gene Families : Cloning and Transgenesis of Large Tandemly-Repeated Histone Gene Clusters Michael P. Meers, Mary Leatham-Jensen, Taylor J.R. Penke, Daniel J. McKay, Robert J. Duronio, and A. Gregory Matera 18. Imaging and Quantitation of Assembly Dynamics of the Centromeric Histone H3 Variant CENP-A in Drosophila melanogaster Spermatocytes by Immunofluorescence and Fluorescence In-Situ Hybridization (Immuno-FISH) Caitriona M. Collins and Elaine M. Dunleavy

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詳細情報

• NII書誌ID(NCID): BB27084614
• ISBN:
  • 9781493986620
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York
• ページ数/冊数: xii, 359 p.
• 大きさ: 26 cm
• 親書誌ID:
  • BA88873632
  • BA10794673