Plant pathogenic fungi and oomycetes : methods and protocols
著者
書誌事項
Plant pathogenic fungi and oomycetes : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1848）（Springer protocols）
Humana Press, c2018
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注記
Includes bibliographical reference and index
内容説明・目次
内容説明
This book describes approaches for the analysis of plant pathogenic fungi that range from ecosystem composition to in vitro and wet-lab to computational analyses. Chapters detail bioinformatics protocols for genome assembly, transcriptome analysis and small RNA profiling, characterization of secondary metabolome, analysis of post-translational modification and localization of proteins, examination of virulence function and interaction with plants or other microorganisms, and targeted mutagenesis using various approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Plant Pathogenic Fungi and Oomycetes: Methods and Protocols aims to provide techniques developed through a wide range of perspectives to facilitate research on a comprehensive understanding of these important filamentous pathogens.
目次
1. Quantifying Re-association of a Facultative Endohyphal Bacterium with a Filamentous Fungus
David A. Baltrus, Joseph Spraker, and A. Elizabeth Arnold
2. Characterizing Mycoviruses
Mahtab Peyambari and Marilyn J. Roossinck
3. Analysis of Secondary Metabolites from Plant Endophytic Fungi
Jiajia Liu and Gang Liu
4. Protocols for Investigating the Leaf Mycobiome using High throughput DNA Sequencing
Shawn P. Brown, Devin R. Leopold, and Posy E. Busby
5. Characterizing Small RNAs in Filamentous Fungi using the Rice Blast Fungus, Magnaporthe oryzae, as an Example
Raman Vidhyavathi, Meyers Blake C., Dean Ralph A., and Donofrio Nicole M.
6. Plant Small RNAs Responsive to Fungal Pathogen Infection Yun Jin and Hui-Shan Guo
7. Sequential Phosphopeptide Enrichment for phosphoproteome Analysis of Filamentous Fungi: A Test Case using Magnaporthe oryzae
Yeonyee Oh, William L. Franck, and Ralph A. Dean
8. Assays for MAP kinase Activation in Magnaporthe oryzae and other Plant Pathogenic Fungi
Xue Zhang, Zhuyun Bian, and Jin-Rong Xu
9. Visualizing the Movement of Magnaporthe oryzae Effector Proteins in Rice Cells during Infection
Kiersun Jones and Chang Hyun Khang
10. Illuminating Phytophthora Biology with Fluorescent Protein Tags
Audrey M.V. Ah-Fong, Meenakshi Kagda, and Howard S. Judelson
11. Methods for Transient Gene Expression in Wild Relatives of Potato
Sidrat Abdullah and Dennis Halterman
12. Host-induced Gene Silencing (HIGS) for Elucidating Puccinia Gene Function in Wheat
Chuntao Yin and Scot Hulbert
13. From Short Reads to Chromosome-scale Genome Assemblies
Kyle Fletcher and Richard Michelmore
14. BLASTmap: A Shiny-based Application to Visualise BLAST Results as Interactive Heat Maps and a Tool to Design Gene Specific Baits for Bespoke Target Enrichment Sequencing Katie Baker, Gordon Stephen, Shona Strachan, Miles Armstrong, and Ingo Hein
15. A Computational Protocol to Analyze Meta-transcriptomic Data Capturing Fungal-host Interactions
Yong Zhang, Li Guo, and Li-Jun Ma
16. Application of the Cre/lox System to Construct Auxotrophic Markers for Quantitative Genetic Analyses in Fusarium graminearum
Lanelle R. Connolly, Allyson A. Erlendson, Corinne M. Fargo, Kendra K. Jackson, Morgan M.G. Pelker, Jacob W. Mazzola, Mark S. Geisler, and Michael Freitag
17. Protocol of Phytophthora capsici Transformation using the CRISPR-Cas9 System
Zhiwen Wang, Brett M. Tyler, and Xili Liu
18. Generating Gene Silenced Mutants in Phytophthora sojae
Yao Zhao, Yufei Li, Min Qiu, Wenbo Ma,and Yuanchao Wang
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