Adipose-derived stem cells : methods and protocols
著者
書誌事項
Adipose-derived stem cells : methods and protocols
(Methods in molecular biology / John M. Walker, series editor, 1773)(Springer protocols)
Humana press, c2018
2nd ed
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This second edition volume expands on the previous edition with a comprehensive update on recent developments in the Adipose Stromal/Stem Cells (ASC) field and the use of stroma vascular fraction (SVF) as a potential therapeutic strategy. The chapters in this book discuss protocols that are focused on discovery, preclinical, and clinical processes. Although most chapters focus on human ASC, there are several chapters that look at small and large animal species with pre-clinical relevance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips in troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Adipose-Derived Stem Cells: Methods and Protocols, Second Edition is a valuable resource for anyone interested in learning more about the scientific advances in the field of ASC biology and application.
目次
Preface...Table of Contents...Contributing Authors...
1. Isolation and Flow Cytometric Analysis of the Stromal Vascular Fraction Isolated from Mouse Adipose TissueAnnie C. Bowles, Alan Tucker, and Bruce A. Bunnell
2. A Method for Isolation of Stromal Vascular Fraction Cells in a Clinically Relevant TimeframeJoel A. Aronowitz, Ryan A. Lockhart, and Cloe S. Hakakian
3. Scaffold-Free, Size-Controlled Three-Dimensional Culture of Rabbit Adipose-Derived Stem CellsChristina L. Rettinger, Kai P. Leung, and Rodney K. Chan
4. The Differentiation of Brown Adipocyte Progenitors Derived from Human Induced Pluripotent Stem CellsAnne-Laure Hafner, Tala Mohsen-Kanson, and Christian Dani
5. Methylcellulose Based Thermally Reversible Hydrogels Anoosha Forghani and Ram Devireddy
6. Decellularized Adipose Tissue Scaffolds for Soft Tissue Regeneration and Adipose-Derived Stem/Stromal Cell Delivery Pascal Morissette Martin, Arthi Shridhar, Claire Yu, Cody Brown, and Lauren E. Flynn
7. Induction of Skin Allograft Transplantation Tolerance in Mice Using Human Adipose Derived Stromal Cells Anthony D. Foster, Nicholas Clark, and Thomas A. Davis
8. High Definition Confocal Imaging Modalities for the Characterization of Tissue-Engineered SubstitutesDominique Mayrand and Julie Fradette
9. Adipose-Derived Stromal Vascular Fraction Cells and Platelet-Rich Plasma: Basic and Clinical Implications for Tissue Engineering Therapies in Regenerative Surgery Pietro Gentile and Valerio Cervelli
10. Myogenic Differentiation of ASCs using Biochemical and Biophysical Induction Pinar Yilgor Huri, Jordana Gilbert-Honick, Justin Morrissette-McAlmon, Sarah Somers, and Warren L. Grayson
11. Isolation of Murine Adipose-Derived Stromal/Stem Cells for Adipogenic Differentiation or Flow Cytometry-Based AnalysisGail Kilroy, Marilyn Dietrich, Xiying Wu, Jeffrey M. Gimble, and Z. Elizabeth Floyd
12. Three-Dimensional Magnetic Levitation Culture System Simulating White Adipose Tissue Hubert Tseng, Alexes C. Daquinag, Glauco R. Souza, and Mikhail G. Kolonin
13. Isolation of Human Adipose-Derived Stem Cells from LipoaspiratesJie Li, J. Lowry Curley, Z. Elizabeth Floyd, Xiying Wu, Yuan Di C. Halvorsen, and Jeffrey M. Gimble
14. Isolation of Murine Adipose-Derived Stromal/Stem Cells using an Explant Culture Method Jie Li, Hui Li, and Weidong Tian
15. Feline Adult Adipose Tissue-Derived Multipotent Stromal Cell Isolation and Differentiation Carmel Fargason, Nan Zhang, and Mandi J. Lopez
16. Soft Tissue ReconstructionKacey G. Marra
17. Mechanical Stimulation of Adipose-Derived Stem Cells for Functional Tissue Engineering of the Musculoskeletal System via Cyclic Hydrostatic Pressure, Simulated Microgravity, and Cyclic Tensile Strain Rachel C. Nordberg, Josie C. Bodle, and Elizabeth G. Loboa
18. Cryopreservation Protocols for Human Adipose Tissue Derived Adult Stem Cells Shahensha Shaik and Ram Devireddy
19. Bone Regeneration with a Combination of Adipose-Derived Stem Cells and Platelet-Rich Plasma Satoshi Tajima, Morikuni Tobita, and Hiroshi Mizuno
20. Isolation and Differentiation of Murine Primary Brown/Beige Preadipocytes Hui Yu, Margo Emont, Heejin Jun, and Jun Wu
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