Myogenesis : methods and protocols
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Bibliographic Information
Myogenesis : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1889）（Springer protocols）
Humana Press, c2019
- : [hardcover]
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Note
Includes bibliographical references and index
Description and Table of Contents
Description
This detailed volume collects many of the common experimental approaches used to study myogenesis. It covers subjects ranging from isolation and purification protocols, manipulation of muscle cells, transcriptomics and proteomics, metabolism and exercise, and tissue engineering. Presented methods involve different species, including human, bovine, Atlantic salmon, rats, mice, larval zebrafish, and Drosophila melanogaster. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Myogenesis: Methods and Protocols aims to serve as an essential part of many laboratory libraries and to assist researchers throughout the world in revealing the unknowns of myogenesis.
Table of Contents
1. Purification of Myogenic Progenitors from Human Muscle Using Fluorescence-Activated Cell Sorting (FACS)
Anna Pakula, Janelle M. Spinazzola, and Emanuela Gussoni
2. Electrical Pulse Stimulation of Primary Human Skeletal Muscle Cells
Natasa Nikolic and Vigdis Aas
3. Transdifferentiation of Muscle Satellite Cells to Adipose Cells Using CRISPR/Cas9-Mediated Targeting of MyoD
Jingjuan Chen, Chao Wang, and Shihuan Kuang
4. Chromatin Immunoprecipitation in Skeletal Myoblasts
Vinay Kumar Rao, Shilpa Rani Shankar, and Reshma Taneja
5. Exercising Bioengineered Skeletal Muscle In Vitro: Biopsy to Bioreactor
Daniel C. Turner, Andreas M. Kasper, Robert A. Seaborne, Alexander D. Brown, Graeme L. Close, Mark Murphy, Claire E. Stewart, Neil R.W. Martin, and Adam P. Sharples
6. Isolation and Purification of Satellite Cells from Young Rats by Percoll Density Gradient Centrifugation
Yuji Matsuyoshi, Mariko Akahoshi, Mako Nakamura, Ryuichi Tatsumi, and Wataru Mizunoya
7. Lentivirus-Mediated RNAi in Skeletal Myogenesis
Dongwook Kim, Adriana Reyes-Ordonez, and Jie Chen
8. Adipogenesis from Bovine Precursors
Frea Mehta, Ruud Theunissen, and Mark J. Post
9. Transcriptomic Profiling during Myogenesis
Alicja Majewska, Tomasz Domoradzki, and Katarzyna Grzelkowska-Kowalczyk
10. Co-Culture Method to Obtain Endothelial Networks within Human Tissue-Engineered Skeletal Muscle
Dacha Gholobova, Melanie Gerard, Lisanne Terrie, Linda Desender, Janet Shansky, Herman Vandenburgh, and Lieven Thorrez
11. Interaction between Skeletal Muscle Cells and Extracellular Matrix Proteins Using a Serum Free Culture System
Danielle E. Dye, Beverley F. Kinnear, Vishal Chaturvedi, and Deirdre R. Coombe
12. LC-MS Analyses of Lipid Species in Skeletal Muscle Cells and Tissue
Marta Moreno-Torres, Jesper F. Havelund, and Nils J. Faergeman
13. siRNA Gene Silencing during C2C12 Myogenesis
Alexis Parente, Luce Perie, Laetitia Magnol, Khaled Bouhouche, and Veronique Blanquet
14. Fluorescence-Activated Cell Sorting of Larval Zebrafish Muscle Stem/Progenitor Cells Following Skeletal Muscle Injury
Dhanushika Ratnayake and Peter D. Currie
15. Preparation of Proliferated Bovine Primary Skeletal Muscle Cells for Bottom-Up Proteomics by LC-MSMS Analysis
Eva Veiseth-Kent, Vibeke Host, and Mona E. Pedersen
16. Myogenesis in Drosophila melanogaster: Dissection of Distinct Muscle Types for Molecular Analysis
Anton L. Bryantsev, Lizzet Castillo, Sandy T. Oas, Maria B. Chechenova, Tracy Dohn, and TyAnna L. Lovato
17. Measuring Both Glucose Uptake and Myosin Heavy Chain Isoform Expression in Single Rat Skeletal Muscle Fibers
Mark W. Pataky, Edward B. Arias, and Gregory D. Cartee
18. Myoblast Phosphoproteomics as a Tool to Investigate Global Signalling Events during Myogenesis
Fiona K. Jones, Gemma E. Hardman, Samantha Ferries, Claire E. Eyers, and Addolorata Pisconti
19. Preparation and Culturing of Atlantic Salmon Muscle Cells for In Vitro Studies
Tone-Kari K. Ostbye and Elisabeth Ytteborg
20. RNA Interference Screening for Genes Regulating Drosophila Muscle Morphogenesis
Aynur Kaya-Copur and Frank Schnorrer
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