Rapid cycle real-time PCR : methods and applications
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書誌事項
Rapid cycle real-time PCR : methods and applications
Springer, c2001
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注記
Includes bibliographical references
内容説明・目次
内容説明
The first comprehensive treatise on Rapid Cycle Real-Time PCR. With amplification times of 15-30 minutes of on-line detection and analysis, nucleic acid quantification of mutation analysis finally becomes a routine, powerful and rapid method. Focusing primarily on the LightCycler, an instrument that combines Rapid Cycle PCR with fluorescent monitoring, this technology provides convenient analysis by melting temperatures. PCR products can be identified by product Tm, and single base mismatches can easily be genotyped by probe Tm. Methods chapters detail the theory behind quantification of mutation analysis; the design of synthesis of fluorescent hybridization probes of the preparation of template DNA. Application chapters apply nucleid acid quantification to infectious organisms of intracellular messengers and mutation detection to somatic of acquired mutations.
目次
Rapid Cycle Real-Time PCR: Methods and Applications.- I Methods.- Mutation Detection by Fluorescent Hybridization Probe Melting Curves.- Quantification on the LightCycler.- Selection of Hybridization Probes for Real-Time Quantification and Genetic Analysis.- Using the Nearest Neighbor Model for the Estimation of Matched and Mismatched Hybridization Probe Melting Points and Selection of Optimal Probes on the LightCycler.- Quantification of Human Papilloma Virus Type 16 Using Quantitative Competitive PCR on the LightCycler.- Use of TaqStart Antibody to Increase the Sensitivity of Herpesvirus Quantitative PCR on the LightCycler.- II Genotyping of Human Germline Variations.- High-Speed Detection of ?1-Antitrypsin Deficiency Alleles Pi*S and Pi*Z on the LightCycler.- High-Speed Methylenetetrahydrofolate Reductase C ? T 677 Mutation Detection on the LightCycler.- Dual Color Detection of Splice Variants of the c-erbA ? (Thyroid Hormone Receptor ?) Gene.- Detection of Three Major Polymorphisms in the N-Acetyltransferase 2 Gene by Melting Peak Analysis Using Fluorogenic Hybridization Probes.- Genotyping of Cytochrome P450 2D6*4 Mutation with Fluorescent Hybridization Probes Using LightCycler.- Fluorescent Hybridization Probe Detection of the F508de1 Cystic Fibrosis Allele on the LightCycler.- Genotyping ?-globin Mutations (Hb S, Hb C, Hb E) by Multiplexing Probe Color and Melting Temperature.- Simultaneous Detection of C282Y and H63D Hemochromatosis Mutations Using LCRed 640 and LCRed 705 Labeled Hybridization Probes.- Genotyping of Angiotensin-Converting Enzyme and Angiotensinogen Polymorphisms with the LightCycler System.- Genotyping of the Most Common Thiopurine Methyltransferase Mutations with the LightCycler.- Detection of the Mitochondria) DNA Mutation MELAS3243 Using Hybridization Probes.- III Acquired Genetic Alterations in Human Diseases.- Detection of p53 Allele Deletions in Human Cancer by Quantification of Genomic Copy Number.- Monitoring of Residual Disease in Patients with Chronic Myelogenous Leukemia Using Specific Fluorescent Hybridization Probes for Real-Time Quantitative RT-PCR.- Development of Quantitative RT-PCR for the Expression of Wilms' Tumor WT1 Suppressor Gene in Leukemia on the LightCycler.- Real-Time Detection of Minimal Residual Disease by Amplifying Immunoglobulin Genes in Acute Lymphoblastic Leukemia on the LightCycler.- HERZ/neu Gene Amplification Quantified by PCR and Melting Peak Analysis Using a Single Base Alteration Competitor as an Internal Standard.- Quantification of Residual Tumor Cells in Monoclonal B-cell Lymphoma.- Development of PCR-Based Assays for the Detection of Chromosomal Translocations Using SYBR Green I.- Relative Quantification of the HER2/neu Oncogene Using SYBR Green I.- IV Receptors and Mediators.- Development of Quantitative RT-PCR Tests for the Expression of Cytokine Genes on the LightCycler.- Quantitative RT-PCR for the Detection of T Cell Receptor Transcripts in T Lymphocytes Populations Using LightCycler Technology.- Rapid, Homogeneous Genotyping of Human Platelet Antigen 1 by Fluorescence Resonance Energy Transfer and Probe Melting Curves.- Development and Validation of an Externally Standardised Quantitative Insulin-like Growth Factor-1 RT-PCR Using LightCycler SYBR Green I Technology.- An Application of Melting Curve Analysis to Large-Scale Genetic Analysis in Atherosclerotic Disease: Two Linked Polymorphisms of Glycoprotein la Gene and Myocardial Infarction in Japanese.- V Infectious Organisms.- Genotype-Specific Analysis of Hepatitis B Virus DNA on the LightCycler.- Rapid and Specific Detection of Bordetella pertussis in Clinical Specimens by LightCycler PCR.- Rapid and Specific Detection of Helicobacter pylori by LightCycler PCR.- Qualitative Detection of Herpes Simplex Virus DNA on the LightCycler.- Quantitative Detection of Cryptosporidium parvum after In Vitro Excystation by LightCycler PCR.- Quantitative Analysis of CMV in Infected Mice on the LightCycler System.- Detection and Differentiation of Equine Herpes Virus Type 1 and Type 4 on the LightCycler.- Rapid and Quantitative Detection of Toxoplasma gondii by PCR - A LightCycler Application in Prenatal Diagnosis.- Development of Quantitative PCR Tests for the Detection of the Orthopox Virus Adsorption Protein Gene (ORF D8L) on the LightCycler.- VI Plant Gene Products and Miscellaneous.- Quantification of Genetically Modified Soybeans in Food with the LightCycler System.- Real-Time PCR Monitoring of Estuarine Water Samples for Pfiesteria piscicida: A Dinoflagellate Associated with Fish Kills and Human Illness.- Quantification of Retrotransposon XIR-2.5 Copy Number in Genomes of Poeciliidae Species.
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