Pre-clinical models : techniques and protocols
Author(s)
Bibliographic Information
Pre-clinical models : techniques and protocols
（Methods in molecular biology / John M. Walker, series editor, 1916）（Springer protocols）
Humana Press, c2019
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Includes bibliographical references and index
Description and Table of Contents
Description
This volume details reviews and protocols on the development and analysis of both cellular and animal-based pre-clinical models in a number of medical areas, including metabolic disorders, longevity, cancer, heart disease and psychiatric disorders. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Pre-Clinical Models: Techniques and Protocols aims to provide methods that describe the context of specific disease or therapeutic areas.
Table of Contents
- Part I: Reviews 1. A Guide to Mass Spectrometry-based Quantitative Proteomics Bradley J. Smith, Daniel Martins-de-Souza, and Mariana Fioramonte 2. Mitochondrial Involvement in Mental Disorders
- Energy Metabolism, Genetic and Environmental Factors Keiko Iwata 3. Monosodium Glutamate (MSG)-induced Animal Model of Type 2 Diabetes Zahra Bahadoran, Parvin Mirmiran, and Asghar Ghasemi Part II: Protocols 4. The Elevated Plus Maze Test for Measuring Anxiety-like Behaviour in Rodents Ann-Katrin Kraeuter, Paul C. Guest, and Zoltan Sarnyai 5. The Forced Swim Test for Depression-like Behaviour in Rodents Ann-Katrin Kraeuter, Paul C. Guest, and Zoltan Sarnyai 6. Object Burying Test for Assessment of Obsessive Compulsive Behaviours in Mice Ann-Katrin Kraeuter, Paul C. Guest, and Zoltan Sarnyai 7. The Nest Building Test in Mice for Assessment of General Well-being Ann-Katrin Kraeuter, Paul C. Guest, and Zoltan Sarnyai 8. Mouse Free Dyadic Social Interaction Test Ann-Katrin Kraeuter, Paul C. Guest, and Zoltan Sarnyai 9. The Open Field Test for Measuring Locomotor Activity and Anxiety-Like Behaviour Ann-Katrin Kraeuter, Paul C. Guest, and Zoltan Sarnyai 10. The Y-maze for Assessment of Spatial Working and Reference Memory in Mice Ann-Katrin Kraeuter, Paul C. Guest, and Zoltan Sarnyai 11. Maturation of a Human Oligodendrocyte Cell Line Gabriela Seabra, Valeria de Almeida, and Daniel Martins-de-Souza 12. Monitoring Age-related Changes in the Lactate/pyruvate Ratio using a Colorimetric Assay in a C. elegans Model of Increased Lifespan Sumino Yanase, Kayo Yasuda, and Naoki Ishii 13. Isolation and Characterisation of Different Mesenchymal Stem Cell Populations from Rat Femur Frans Alexander Jacobs, Mari van de Vyver, and William Frank Ferris 14. Characterization of an Animal Model of Autism and Social Interaction Keiko Iwata 15. Assaying Reproductive Capacity in Female Rodents Catherine E. Aiken, Jane L. Tarry-Adkins,and Susan E. Ozanne 16. Experimental Model of Hindlimb Suspension-Induced Skeletal Muscle Atrophy in Rodents Gabriel Nasri Marzuca-Nassr, Kaio Fernando Vitzel, Gilson Masahiro Murata, Jose Luis Marquez, and Rui Curi 17. An Analysis of the Intracellular Signal Transduction of Peripheral Blood Leukocytes in Animal Models of Diabetes using Flow Cytometry Yuji Takeda, Hironobu Asao, and Ichiro Wakabayashi 18. Characterization of the db/db Mouse Model of Type 2 Diabetes Paul C Guest and Hassan Rahmoune 19. Characterization of the Goto-Kakizaki (GK) Rat Model of Type 2 Diabetes Paul C. Guest 20. Characterization of Human Transplantable Insulinoma Cells Paul C. Guest 21. Preparation of Islets from Rat Pancreas and Assessment of Islet Function Paul C Guest 22. Cryopreservation of Red Blood Cells Noha AS Alotaibi, Nigel KH Slater, and Hassan Rahmoune 23. Molecular Characterization of Human Leukemia 60 (HL-60) Cells as a Model of Acute Myelogenous Leukemia Post Cryo-preservation Noha AS Al-Otaibi, Juliana S Cassoli, Nigel KH Slater, and Hassan Rahmoune 24. Reprogramming of Human Melanocytes and Melanoma cells with Yamanaka Factors Hadiseh Taheri, Umut Cagin, and Acelya Yilmazer 25. The Use of 3T3-L1 Murine Preadipocytes as a Model of Adipogenesis Eleanor Cave and Nigel J Crowther 26. Generation of Human Pyruvate Carboxylase Knockout Cell Lines Using Retrovirus Expressing Short Hairpin RNA and CRISPR-Cas9 as Models to Study its Metabolic Role in Cancer Research Khanti Rattanapornsompong, Jarunya Ngamkham, Tanit Chavalit, and Sarawut Jitrapakdee 27. The use of Primary Hepatocytes in Assessment of Drug Safety and Toxicity Paul C. Guest 28. OVA-induced Allergic Airway Inflammation Mouse Model Mateus Casaro, Vanessa R. Souza, Fernando A. Oliveira, and Caroline M. Ferreira 29. Description of Ovariectomy Protocol in Mice Vanessa R. Souza, Eduardo Mendes, Mateus Casaro, Ana Tada F.B. Antiorio, Fernando A. Oliveira, and Caroline M. Ferreira 30. A Rat Eye Lens Model of Cataract Formation Paul C. Guest 31. A Complete Proteomic Workflow to Study Brain-Related Disorders via Postmortem Tissue Guilherme Reis-de-Oliveira, Mariana Fioramonte, and Daniel Martins-de-Souza 32. Method Protocols for Metabolic and Functional Analysis of the BRIN-BD11 -Cell Line- A Pre-clinical Model for Type 2 Diabetes Jordan Rowlands, Nikita Walz, Joanne E. Rowles, Kevin N. Keane, Rodrigo Carlessi, and Philip Newsholme
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