Epigenome editing : methods and protocols

著者

• Jeltsch, Albert
• Rots, Marianne G.

書誌事項

Epigenome editing : methods and protocols

edited by Albert Jeltsch, Marianne G. Rots

（Methods in molecular biology / John M. Walker, series editor, 1767）（Springer protocols）

Humana Press, c2018

内容説明・目次

内容説明

This detailed book explores the concepts and applications of epigenome editing, as presented by leading scientists in the field. Beginning with some general and topical reviews, the collection continues by covering the design of DNA-binding devices, optimization of the effector domains, readout of epigenome marks, and approaches for delivery at the cellular and organismal level. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Epigenome Editing: Methods and Protocols will be of great assistance to people new to the field but also to those already engaged, as epigenetic editing is still a relatively unexplored field with many issues to be resolved.

目次

Part I: General Reviews 1. Editing the Epigenome: Overview, Open Questions, and Directions of Future Development Marianne G. Rots and Albert Jeltsch 2. Zinc Fingers, TALEs and CRISPR Systems: A Comparison of Tools for Epigenome Editing Charlene Babra Waryah, Colette Moses, Mahira Arooj, and Pilar Blancafort 3. Designing Epigenome Editors: Considerations of Biochemical and Locus Specificities Dilara Sen and Albert J. Keung 4. Generation of TALE-Based Designer Epigenome Modifiers Sandra Nitsch and Claudio Mussolino Part II: Topical Reviews 5. Neuroepigenetic Editing Peter J. Hamilton, Carissa J. Lim, Eric J. Nestler, and Elizabeth A. Heller 6. Allele-Specific Epigenome Editing Pavel Bashtrykov and Albert Jeltsch 7. Key to Delivery: The (Epi-)Genome Editing Vector Toolbox Sabrina Just and Hildegard Buning 8. CRISPR/dCas9 Switch Systems for Temporal Transcriptional Control Rutger A.F. Gjaltema and Edda G. Schulz Part III: Delivery Protocols 9. Delivery of Designer Epigenome Modifiers into Primary Human T Cells Tafadzwa Mlambo, Marianna Romito, Tatjana I. Cornu, and Claudio Mussolino 10. Viral Expression of Epigenome Editing Tools in Rodent Brain Using Stereotaxic Surgery Techniques Peter J. Hamilton, Carissa J. Lim, Eric J. Nestler, and Elizabeth A. Heller 11. Stable Expression of Epigenome Editors Via Viral Delivery and Genomic Integration Carolin Kroll and Philipp Rathert 12. Purified-Protein Delivery to Activate an Epigenetically-Silenced Allele in Mouse Brain Benjamin Pyles, Barbara J. Bailus, Henriette O'Geen, and David J. Segal 13. Non-Viral Methodology for Efficient Co-Transfection Jessica A. Kretzmann, Cameron W. Evans, Marck Norret, Pilar Blancafort, and K. Swaminathan Iyer Part IV: Histone Editing Readout Protocols 14. Chromatin Immunoprecipitation in Human and Yeast Cells Jessica B. Lee and Albert J. Keung 15. Chromatin Immunoprecipitation and High Throughput Sequencing (ChIP-Seq): Tips and Tricks Regarding the Laboratory Protocol and Initial Downstream Data Analysis Darren K. Patten, Giacomo Corleone, and Luca Magnani Part V: DNA Methylation Editing Readout Protocols 16. Generation of Whole Genome Bisulfite Sequencing Libraries for Comprehensive DNA Methylome Analysis Dulce B. Vargas-Landin, Jahnvi Pfluger, and Ryan Lister 17. Approaches for the Analysis and Interpretation of Whole Genome Bisulfite Sequencing Data Tim Stuart, Sam Buckberry, and Ryan Lister 18. Whole Genome Bisulfite Sequencing for the Analysis of Genome-Wide DNA Methylation and Hydroxymethylation Patterns at Single-Nucleotide Resolution Magali Kernaleguen, Christian Daviaud, Yimin Shen, Eric Bonnet, Victor Renault, Jean-Francois Deleuze, Florence Mauger, and Joerg Tost 19. Locus-Specific DNA Methylation Analysis by Targeted Deep Bisulfite Sequencing Elsa Leitao, Jasmin Beygo, Michael Zeschnigk, Ludger Klein-Hitpass, Marcel Bargull, Sven Rahmann, and Bernhard Horsthemke 20. DNA Methylation Analysis by Bisulfite Conversion Coupled to Double Multiplexed Amplicon-Based Next-Generation Sequencing (NGS) Pavel Bashtrykov and Albert Jeltsch Part VI: Gene Expression Readout Protocols 21. Cell-to-Cell Transcription Variability as Measured by Single Molecule RNA FISH to Detect Epigenetic State Switching William Beckman, Ilona M. Vuist, Hermannus Kempe, and Pernette J. Verschure 22. Establishment of Cell Lines Stably Expressing dCas-Fusions to Address Kinetics of Epigenetic Editing Desiree Goubert, Mihaly Koncz, Antal Kiss, and Marianne G. Rots Part VII: Special Application Protocols 23. Editing of DNA Methylation Using dCas9-Peptide Repeat and scFv-TET1 Catalytic Domain Fusions Sumiyo Morita, Takuro Horii, and Izuho Hatada 24. Chemical Inducible dCas9-Guided Editing of H3K27 Acetylation in Mammalian Cells Dan Gao and Fu-Sen Liang 25. Screening Regulatory Element Function with CRISPR/Cas9-Based Epigenome Editing Tyler S. Klann, Gregory E. Crawford, Timothy E. Reddy, and Charles A. Gersbach

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詳細情報

• NII書誌ID(NCID): BB27851123
• ISBN:
  • 9781493977734
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York
• ページ数/冊数: xiv, 482 p.
• 大きさ: 26 cm
• 件名:
  • LCSH : Epigenetics -- Laboratory manuals
• 親書誌ID:
  • BA10794673
  • BA88873632