Lactic acid bacteria : methods and protocols
著者
書誌事項
Lactic acid bacteria : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1887）（Springer protocols）
Humana Press, c2019
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内容説明・目次
内容説明
This detailed book provides a collection of protocols for numerous experimental approaches perfected by the authors for lactic acid bacteria (LAB) research. Split in to three parts, the volume delves into the identification and metabolism of LABs, the applications of the bacteria for the food industry, as well as healthy functions of LAB. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Lactic Acid Bacteria: Methods and Protocols serves as an ideal inspiration for many research efforts in the domains of food science and health science.
目次
I Isolation, identification and metabolisms of LAB 1. Isolation and identification of LAB Preface:
Protocols
1) Classification/identifications of LAB
2) Method of homology analysis of DNA
3) Comparisons of characteristics of morphology, physiology ......etc.
2. Metabolism of LAB 1) Method of analyzing metabolisms of LAB
2) Method of assaying enzyme relative metabolisms.
3) Expression of DNA coding enzymes relative metabolisms ......etc.
3. Survival lactic acid bacteria in the human stomach and intestines 1) Method of analyzing LAB in human stomach and intestines
2) Analysis of mechanisms of Survival LAB under the stress conditions.
3) Expression of DNA coding protein relative stress ......etc.
4. The proteolytic systems of lactic acid bacteria 1) Method of assaying proteolytic enzymes.
2) Method of cloning of proteolytic enzymes in LAB.
3) Expression of DNA coding proteolytic enzymes in LAB ......etc.
5. Stress responses in lactic acid bacteria 1) Method of analyzing metabolisms of LAB under stress tolerance.
2) Method of assaying metabolisms under the stress conditions.
3) Expression of DNA coding resistant protein ......etc.
6. Lactic acid bacteria in marine 1) Method of analyzing marine LAB.
2) Method of DGGE analysis marine LAB.
3) Method of homology analysis of DNA of marine LAB.
4) Comparisons of characteristics of morphology, physiology of marine LAB.
7. Responses of lactic acid bacteria to oxygen 1) Method of analyzing metabolisms of LAB under oxygen condition.
2) Method of assaying metabolisms under oxygen condition.
3) Expression of DNA coding resistant protein ......etc.
8. Spore-forming lactic acid bacteria 1) Method of analyzing spore forming of the LAB.
2) Method of analyzing DNA coding spore formation......etc.
Section II -Application of industry of lactic acid bacteria- 9. Applications of tannase in lactic acid bacteria for wine or beer production 1) Method of screening high tannase LAB
2) Method of assaying tannase in LAB.
3) Method of cloning tannase in LAB. ......etc.
10. Roles of hydroxyl fatty acid converting LAB for alcohol beverages production and it conversion mechanisms. 1) Method of screening LAB producing high hydroxyl fatty acid.
2) Method of assaying hydroxyl fatty acid in LAB.
3) Roles of hydroxyl fatty acid under stress conditions.
4) Method of cloning hydroxylase. ......etc.
11. Yogurt production 1) Method of analysis of yogurt quality.
2) Method of assessing LAB ......etc.
12. Cheese production 1) Method of analysis of cheese curd quality.
2) Method of assessing LAB for cheese curd......etc.
13. Elimination of fishy order by LAB and their applying. Preface:
1) Method of screening LAB eliminating fishy order.
2) Method of assaying fishy order.
3) Application of fishy order eliminating LAB. ......etc.
14. Using LAB in Bakery (Sour dough or Panettone Bread) 1) Method of analysis of bread quality.
2) Method of analysis of aroma in bread.
3) Method of assessing LAB for bread......etc.
15. LAB having Hop resistance 1) Method of analysis of hope resistant LAB.
2) Method of analysis of hope resistant system.
3) Method of assessing hop resistance protein and coding their DNA......etc.
16. Meat and Sausages fermentation of LAB 1) Method of isolation of LAB from specific condition as Sausages.
2) Method of analysis of LAB resistant specific condition as Sausages.
3) Method of assessing protein (enzyme?) to grow specific condition...and coding their DNA...etc.
17. Oenococcus oeni 1) Method of analyzing metabolisms of Onenococcus sp.
2) Method of analyzing alcohol tolerance.
3) Expression of DNA coding malate-lactate fermentation......etc.
18. Feed productions 1) Method of isolation LAB in silage
2) Method of DGGE analysis silage LAB
3) Method of analysis of enzyme or metabolisms in silage LAB.
4) Comparisons of characteristics of morphology, physiology of silage
19. LAB using traditional fermented food 1) Method of isolation LAB.
2) Method of TGGE or DGGE analysis LAB
3) Comparisons of characteristics of morphology, physiology of LABs.....etc
Section III -Production of LAB- 20. Production of bacteriocin by LAB 1) Method of screening LAB producing bacteriocin.
2) Method of purification of bacteriocin.
3) Analysis of bacteriocin and LAB.....etc
21. Amino acid racemase in LAB 1) Method of screening LAB converting D-amino acid from L-amino acid.
2) Method of purification of racemase.
3) Characteristics of racemase......etc
22. Immuno-potentiative action substance of LAB 1) Method of screening LAB having immuno-potentiative action.
2) Method of purification of immuno-potentiative acting substance.
3) Analysis of immuno-potentiative acting substance.....etc
23. Polysaccharide production by LAB 1) Method of screening LAB producing exopolysaccharide.
2) Analysis of exopolysaccharide and their LAB.....etc
24. Producing gamma-aminobutyric acid by LAB 1) Method of screening LAB producing GABA.
2) Method of purification of glutameta decarboxylase.
3) Cloning of glutameta decarboxylase in LAB.....etc
25. Production of Menaquinones by Lactic Acid Bacteria 1) Method of screening LAB producing menaquinones.
2) Method of purification of enzyme producing menaquinones.
3) Cloning of enzymes producing menaquinones in LAB.....etc
26. Improved screening procedure for biogenic amine production by lactic acid bacteria 1) Assaying biogenic amine produced by LAB.
2) Screening LAB producing biogenic amine.
3)< Cloning of the enzyme producing biogenic amine in LAB.....etc
Section IV -Healthy function of LAB- 27. Adhesion of LAB with intestinal and bowel wall 1) Method of screening adhesion of LAB with intestinal and bowel wall.
2) Method of analyzing adhesion protein in LAB.
3) Analyzing of adhesion system of LAB and intestinal/bowel wall.....etc
28. Elimination of inflammatory substance as LPS by LAB (Ms Asami and Kanauchi) 1) Method of screening LAB eliminating LPS.
2) Method of analyzing eliminating LPS.
3) Analyzing of eliminating LPS system.....etc
29. Roles of surface protein HSP in LAB ~LPS decomposition 1) Method of Eliminating LPS by HSP.
2) Method of Cloning of HSP.
3) Analyzing of HSP in LAB.....etc
30. Absorption of heavy metal by LAB and excretion of heavy metal from human body. 1) Method of absorption of heavy metal by LAB.
2) Mechanisms of absorption of heavy metal by LAB
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