Plant senescence : methods and protocols

著者

    • Guo, Yongfeng

書誌事項

Plant senescence : methods and protocols

edited by Yongfeng Guo

（Methods in molecular biology / John M. Walker, series editor, 1744）（Springer protocols）

Humana Press, c2018

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

This detailed volume covers a wide variety of techniques either developed specially for plant senescence studies or optimized for studying senescing plants. After an introduction to the topic, the book continues with sections on phenotypic analysis and molecular markers of plant organ senescence, hormonal control of plant senescence, stress-induced senescence, molecular and cellular processes in plant senescence, as well as systems biology approaches. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Senescence: Methods and Protocols aims to provide a useful hand book of standard protocols for plant molecular biologists working on senescence.

目次

Part I: Introduction of Plant Senescence 1. Concepts and Types of Senescence in Plants Susheng Gan 2. Initiation, Progression, and Genetic Manipulation of Leaf Senescence Akhtar Ali, Xiaoming Gao, and Yongfeng Guo Part II: Phenotypic Analysis and Molecular Markers of Plant Organ Senescence 3. Phenotypic Analysis and Molecular Markers of Leaf Senescence Liming Zhao, Yan Xia, Xiao-Yuan Wu, Jos H.M. Schippers, and Hai-Chun Jing 4. Investigation of Petal Senescence by TRV-Mediated Virus-Induced Gene Silencing in Rose Chenxia Cheng, Junping Gao, and Nan Ma 5. Phenotypic Analysis and Molecular Markers of Plant Nodule Senescence Xiuying Xia 6. Quantitative Analysis of Floral Organ Abscission in Arabidopsis via a Petal Breakstrength Assay Chun-Lin Shi and Melinka A. Butenko 7. Characterization of Climacteric and Non-Climacteric Fruit Ripening Xiaohong Kou and Mengshi Wu Part III: Hormonal Control of Plant Senescence 8. Ethylene Treatment in Studying Leaf Senescence in Arabidopsis Zhonghai Li and Hongwei Guo 9. The Assay of Abscisic Acid-Induced Stomata Movement in Leaf Senescence Yanyan Zhang and Kewei Zhang 10. The EPR Method for Detecting Nitric Oxide in Plant Senescence Aizhen Sun 11. Hormone Treatments in Studying Leaf Senescence Zenglin Zhang and Yongfeng Guo Part IV: Stress-Induced Senescence in Plants 12. Methods to Study Darkness-Induced Leaf Senescence Yi Song and Lin Li 13. Salt Treatments and Induction of Senescence Yasuhito Sakuraba, Dami Kim, and Nam-Chon Paek 14. Methods for Elucidation of Plant Senescence in Response to C/N-Nutrient Balance Shoki Aoyama, Junji Yamaguchi, and Takeo Sato 15. Study of Cotton Leaf Senescence Induced by Alternaria alternata Infection Wei Liu, Wenwei Zhang, Na Zheng, Weibo Zhai, and Fangjun Qi 16. Study of Hydrogen Peroxide as a Senescence-Inducing Signal Stefan Bieker, Maren Potschin, and Ulrike Zentgraf 17. Identification of Postharvest Senescence Regulators through Map-Based Cloning Using Detached Arabidopsis Inflorescences as a Model Tissue Donald A. Hunter, Rubina Jibran, Paul Dijkwel, David Chagne, Kerry Sullivan, Aakansha Kanojia, and Ross Crowhurst Part V: Molecular and Cellular Processes in Plant Senescence 18. Chlorophyll and Chlorophyll Catabolite Analysis by HPLC Aditi Das, Luzia Guyer, and Stefan Hoertensteiner 19. Identification and Functional Analysis of Senescence-Associated Genes in Wheat Geng Wang, Ke Li, and Chunjiang Zhou 20. Isolation, Purification, and Detection of MicroRNAs in Plant Senescence Yujun Ren and Ying Miao 21. In Situ Detection of Programmed Cell Death in Senescing Nicotiana tabacum Leaves Using TUNEL Assay Branka Uzelac, Dusica Janosevic, and Snezana Budimir 22. Activities of Vacuolar Cysteine Proteases in Plant Senescence Dana E Martinez, Lorenza Costa, and Juan Jose Guiamet 23. Study of Autophagy in Plant Senescence Xuefei Cui, Jing Zheng, Jinxin Zheng, and Qingqiu Gong 24. Isolation of Chloroplasts for In Organelle Protein Degradation Assay Scott W. Vande Wetering and Judy A. Brusslan 25. Plant Cell Walls: Isolation and Monosaccharide Composition Analysis Yingzhen Kong, Malcolm O'Neill, and Gongke Zhou 26. Visualizing Morphological Changes of Abscission Zone Cells in Arabidopsis by Scanning Electron Microscope Chun-Lin Shi and Melinka A. Butenko Part VI: Systems Biology in Plant Senescence 27. RNA-Seq Analysis of the Transcriptome of Leaf Senescence in Tobacco Wei Li and Yongfeng Guo 28. Comprehensive Metabolomics Studies of Plant Developmental Senescence Mutsumi Watanabe, Takayuki Tohge, Salma Balazadeh, Alexander Erban, Patrick Giavalisco, Joachim Kopka, Bernd Mueller-Roeber, Alisdair R. Fernie, and Rainer Hoefgen

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