Fibrosis : methods and protocols

著者

• Rittié, Laure

書誌事項

Fibrosis : methods and protocols

edited by Laure Rittié

（Methods in molecular biology, 1627）（Springer protocols）

Humana Press, c2017

注記

Includes bibliographical references and index

内容説明・目次

内容説明

This volume describes state-of-the-art protocols that serve as "recipes" for scientists concentrating on fibrosis research. This book is divided into four sections. Part I focuses on animal models of fibrosis and covers topics such as mimicking fibrosis in the lungs, skin, liver and heart, and generating transgenic mouse models. Part II discusses cell culture systems, where the chapters explore cell types important for the development of fibrosis. Part III looks at the purification, quantification, and analysis of the ECM proteins, and Part IV describes computer-assisted methods such as quantifying fibrillar collagen alignment and exploring the nano-surface of collagen with atomic force microscopy (AFM). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Fibrosis: Methods and Protocols is a valuable resource aimed at outstanding quality and repeatability of research experiments in the fibrosis field.

目次

Preface... Table of Contents... Contributing Authors... Part I: Perspective 1. Human Fibrotic Diseases: Current Challenges in Fibrosis Research Joel Rosenbloom, Edward Macarak, Sonsoles Piera-Velazquez, and Sergio A. Jimenez Part II: Animal Models of Fibrosis 2. The Bleomycin Model of Pulmonary Fibrosis Tianju Liu, Francina Gonzalez De Los Santos, and Sem H. Phan 3. Intra-Dermal Injections of Bleomycin to Model Skin Fibrosis Toshiyuki Yamamoto 4. Assessing the Effects of Fibrosis on Lung Function by Light Microscopy-Coupled Stereology Bartosz Pilecki and Grith Lykke Sorensen 5. Transplanting Human Skin Grafts onto Nude Mice to Model Skin Scars Jie Ding and Edward E. Tredget 6. Hypertrophic Scarring in the Rabbit Ear: A Practical Model for Studying Dermal Fibrosis Layla Nabai and Aziz Ghahary 7. Mouse and Rat Models of Induction of Hepatic Fibrosis and Assessment of Portal Hypertension Sabine Klein, Robert Schierwagen, Frank Erhard Uschner, and Jonel Trebicka 8. Mouse Models of Corneal Scarring Laure Rittie, Audrey E.K. Hutcheon, and James D. Zieske 9. Modeling Cardiac Fibrosis in Mice: (Myo)Fibroblast Phenotype after Ischemia Daniela Fraccarollo, Paolo Galuppo, and Johann Bauersachs 10. Characterization of Mesenchymal-Fibroblast Cells using the Col1a2 Promoter/Enhancer Ian M. H. Li, Amy L. Horwell, Grace Chu, Benoit de Crombrugghe, and Goerge Bou-Gharious< Part III: Cell Culture Models 11. Isolation and Culture of Primary Murine Hepatic Stellate Cells Sabine Weiskirchen, Carmen G. Tag, Sibille Sauer-Lehnen, Frank Tacke, and Ralf Weiskirchen 12. Isolation and Culture of Adipose-Derived Stromal Cells from Sub-Cutaneous Fat Lawrence J. Liew, Huan Ting Ong, and Rodney J. Dilley 13. Isolation of Live Fibroblasts by Fluorescence-Activated Cell Sorting Tripp Leavitt, Michael S. Hu, and Michael T. Longaker 14. Detection of Infiltrating Mast Cells using a Modified Toluidine Blue Staining Nahum Puebla-Osorio, Seri N. E. Sarchio, Stephen E. Ullrich, and Scott N. Byrne 15. Cell-Populated Collagen Lattice Models Beate Eckes, Fang Wang, Laure Rittie, Gabriele Scherr, and Paola Zigrino 16. Traction Force Measurement using Deformable Microposts Tianfa Xie, Jamar Hawkins, and Yubing Sun 17. Mechanical Deformation of Cultured Cells with Hydrogels Christal A. Worthen, Laure Rittie, and Gary J. Fisher 18. Preparation of Decellularized Lung Matrices for Cell Culture and Protein Analysis Franziska E. Uhl, Darcy E. Wagner, and Daniel J. Weiss Part IV: ECM Proteins -- Purification, Quantification, and Analysis 19. Type I Collagen Purification from Rat Tail Tendons Laure Rittie 20. Purification of Human Plasma/Cellular Fibronectin and Fibronectin Fragments Giampiero Pietrocola, Simonetta Rindi, Giulia Nobile, and Pietro Speziale 21. Laser Capture Microdissection of Tissue Sections for High-Throughput RNA Analysis Julia Hoffmann, Jochen Wilhelm, and Grazyna Kwapiszewska 22. Collagen Quantification in Tissue Specimens Joao Quintas Coentro, Hector Capella-Monsonis, Valeria Graceffa, Zhuning Wu, Anne Maria Mullen, Michael Raghunath, and Dimitrios I. Zeugolis 23. Methods for the Assessment of Active Transforming Growth Factor- in Cells and Tissues Alison E. John, Joanne Porte, Gisli Jenkins, and Amanda L. Tatler 24. Visualizing in Vitro Type I Collagen Fibrillogenesis by Transmission Electron Microscopy J. Robin Harris 25. Histological and Electron Microscope Staining for the Identification of Elastic Fiber Networks Elaine C. Davis and Ling Li 26. Method for Picrosirius Red-Polarization Detection of Collagen Fibers in Tissue Sections Laure Rittie 27. Probing Collagen Organization: Practical Guide for Second Harmonic Generation (SHG) Imaging Riccardo Cicchi and Francesco S. Pavone Part V: ECM Proteins -- Purification, Quantification, and Analysis 28. Methods for Quantifying Fibrillar Collagen Alignment Yuming Liu, Adib Keikhosravi, Guneet S. Mehta, Cole R. Drifka, and Kevin W. Eliceiri 29. Exploring the Nano-Surface of Collagenous and Other Fibrotic Tissues with AFM Andreas Stylianou, Vasiliki Gkretsi, Costas S. Patrickios, and Trianatafyllos Stylianopoulos 30. Spectral Unmixing Methods and Tools for the Detection and Quantitation of Collagen and Other Macromolecules in Tissue Specimens Zachary T. Harmany, Farzad Fereidouni, and Richard M. Levenson 31. Simple Analysis of Deposited Gene Expression Datasets for the Non-Bioinformatician: How to Use GEO for Fibrosis Research Yang Guo, Richard Townsend, and Lam C. Tsoi

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詳細情報

• NII書誌ID(NCID): BB28145292
• ISBN:
  • 9781493971121
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York
• ページ数/冊数: xv, 530 p.
• 大きさ: 26 cm
• 親書誌ID:
  • BA07889873
  • BA88873632