Mycobacteria protocols
著者
書誌事項
Mycobacteria protocols
（Springer protocols）（Methods in molecular biology / John M. Walker, series editor, 1285）
Humana Press, c2015
3rd ed
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Updated and revised, this thorough volume provides a selection of the newest methods, as well as some of the basic methods required for a mycobacterial research laboratory. Mycobacteria Protocols, Third Edition guides readers through fractionation and analysis of macromolecules, from nucleic acids to proteins, complex lipids, and metabolites. Detailed and comprehensive protocols are provided for protein and lipid/glycolipid analysis using well-established methods; these are now complemented by a metabolomics chapter in which the complement of metabolites can be profiled. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Mycobacteria Protocols, Third Edition will be a resource both to those working in the field and to newcomers.
目次
1. Whole Genome Sequencing for Comparative Genomics and De Novo Genome Assembly
Andrej Benjak, Claudia Sala, and Ruben C. Hartkoorn
2. Whole Transcriptome Sequencing for High-resolution Transcriptomic Analysis in Mycobacterium tuberculosis
Andrej Benjak, Claudia Sala, and Ruben C. Hartkoorn
3. RNA Sequencing for Transcript 5'-end Mapping in Mycobacteria
Scarlet S. Shell, Michael R. Chase, Thomas R. Ioerger, and Sarah M. Fortune
4. Fractionation and Analysis of Mycobacterial Proteins
Megan C. Lucas, Lisa M. Wolfe, Rachel M. Hazenfield, Jade Kurihara, Nicole A. Kruh-Garcia, John Belisle, and Karen M. Dobos
5. Lipid and Lipoarabinomannan Isolation and Characterization
Marie-Antoinette Laneelle, Jerome Nigou, and Mamadou Daffe
6. Metabolomics of Mycobacterium tuberculosis
Madhumitha Nandakumar, Gareth A. Prosser, Luis Pedro S. de Carvalho, and Kyu Rhee
7. Electroporation of Mycobacteria
Renan Goude, David M. Roberts, and Tanya Parish
8. Targeted Gene Knockout and Essentiality Testing by Homologous Recombination
Krishnamoorthy Gopinath, Digby F. Warner, and Valerie Mizrahi
9. Construction of Conditional Knockdown Mutants in Mycobacteria
Dirk Schnappinger, Kathryn M. O'Brien, and Sabine Ehrt
10. Mycobacterial Recombineering
Kenan C. Murphy, Kadamba Papavinasasundaram, and Christopher M. Sassetti
11. In vitro Models that Utilize Hypoxia to Induce Non-replicating Persistence in Mycobacteria
Charles D. Sohaskey and Martin I. Voskuil
12. Genetic Dissection of Mycobacterial Biofilms
Anil K Ojha, William R. Jacobs Jr., and Graham F. Hatfull
13. Measuring Efflux and Permeability in Mycobacteria
Liliana Rodrigues, Miguel Viveiros, and Jose A. Ainsa
14. Single-cell Analysis of Mycobacteria using Microfluidics and Time-lapse Microscopy
Neeraj Dhar and Giulia Manina
15. Antimicrobial Susceptibility Testing for Mycobacterium sp.
Delia Blanco-Ruano, David M. Roberts, Ruben Gonzalez-Del-Rio, Daniel Alvarez, M Jose Rebollo, Esther Perez-Herran, and Alfonso Mendoza
16. Determination of Compound Kill Kinetics Against Mycobacterium tuberculosis
Julie Early and Torey Alling
17. Microplate Alamar Blue Assay (MABA) and Low Oxygen Recovery Assay (LORA) for Mycobacterium tuberculosis
Sanghyun Cho, Hyung Sup Lee, and Scott Franzblau
18. A Multi-Stress Model for High Throughput Screening Against Non-replicating Mycobacterium tuberculosis
Ben Gold, Thulasi Warrier, and Carl Nathan
19. Isolation and Characterization of Compound-resistant Isolates of Mycobacterium tuberculosis
Theresa O'Malley and Eduard Melief
20. Macrophage Infection Models for Mycobacterium tuberculosis
Benjamin K. Johnson and Robert B. Abramovitch
21. Infection of Human Neutrophils to Study Virulence Properties of Mycobacterium tuberculosis
Tobias Dallenga, Bjoern Corleis, and Ulrich E. Schaible
22. Isolation of Bead-phagosomes to Study Virulence Function of M. tuberculosis Cell Wall Lipids
Anna Christina Geffken, Emmanuel C. Patin, and Ulrich E. Schaible
23. Live Imaging of Mycobacterium marinum Infection in Dictyostelium discoideum
Caroline Barisch, Ana T. Lopez-Jimenez, and Thierry Soldati
24. Testing Chemical and Genetic Modulators in Mycobacterium tuberculosis Infected Cells using Phenotypic Assays
Vincent Delorme, Ok-Ryul Song, Alain Baulard, and Priscille Brodin
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