Protein chromatography : methods and protocols

著者

• Walls, Dermot
• Loughran, Sinéad T

書誌事項

Protein chromatography : methods and protocols

edited by Dermot Walls, Sinéad T. Loughran

（Methods in molecular biology / John M. Walker, series editor, 1485）（Springer protocols）

Humana Press, c2017

2nd ed

• : pbk

注記

Includes bibliographical references and index

内容説明・目次

内容説明

This second edition expands on the previous edition with new chapters that are suitable for newcomers, as well as more detailed chapters that cover protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the challenges that scale-up of production poses to the investigator. Many of the chapters also discuss generation and purification of recombinant proteins, recombinant antibody production, and the tagging of proteins as a means to enhance their solubility and simplify their purification on an individual scale or in high-throughput systems. This book also provides readers with chapters that describe not just the more commonly used methods, but also recently developed approaches such as proteomic/mass spectrometric techniques and Lectin-based affinity chromatography. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Protein Chromatography: Methods and Protocols, Second Edition is a valuable resource for anyone who is interested in the field of protein chromatography.

目次

Part I Overview Chapters 1. A Synopsis of Protein and their Purification Dermot Walls, Gary Cooney, and Sinead T. Loughran 2. Gel-Filtration Chromatography Ciaran O'Fagain, Philip M. Cummins, and Brendan F. O'Connor 3. Immunoaffinity Chromatography: Concepts and Applications Jenny Fitzgerald, Paul Leonard, Elaine Darcy, Shikha Sharma, and Richard O'Kennedy 4. Avoiding Proteolysis During Protein PurificationBarry J. Ryan and Gary T. Henehan 5. Scale-Up of Protein Purification: Downstream Processing Issues John Joseph Milne 6. Phage Display: A Powerful Technology for the Generation of High Specificity Affinity Reagents from Alternative Immune Sources William J.J. Finlay, Laird Bloom, Joanne Grant, Edward Franklin, Deirdre Ni Shuilleabhain, and Orla Cunningham 7. Protein Stability: Enhancement and Measurement Ciaran O'Fagain 8. Tagging Recombinant Proteins to Enhance Solubility and Aid Purification Sinead T. Loughran and Dermot Walls Part II Protocols Chapters 9. Storage and Lyophilisation of Pure Proteins Ciaran O'Fagain and Keith Colliton < 10. Differential Precipitation and Solubilization of Proteins Barry J. Ryan and Gemma K. Kinsella 11. Ion-Exchange Chromatography: Basic Principles and Application Philip M. Cummins, Keith D. Rochfort, and Brendan F. O'Connor 12. Protein Quantitation and Analysis of Purity Eva M. Campion, Sinead T. Loughran, and Dermot Walls 13. Purification of Proteins Fused to Maltose-Binding Protein Mario Lebendiker and Tsafi Danieli 14. Purification of Poly-Histidine-Tagged Proteins Sinead T. Loughran, Ronan T. Bree, and Dermot Walls 15. Purification of Antibodies Using Affinity Chromatography Elaine Darcy, Paul Leonard, Jenny Fitzgerald, Martin Danaher, Hui Ma, and Richard O' Kennedy 16. Optimized Generation of High-Affinity, High-Specificity Single-Chain Fv Antibodies from Multi-Antigen Immunized Chickens William J.J. Finlay, Laird Bloom, Sreeja Varghese, Benedicte Autin, and Orla Cunningham 17. Measuring Protein-Protein Interactions Using Biacore Paul Leonard, Stephen Hearty, Hui Ma, and Richard O'Kennedy 18. Hydrophobic Interaction Chromatography Brendan F. O'Connor and Philip M. Cummins 19. Fast Protein Liquid Chromatography Ashkan Madadlou, Siobhan O'Sullivan, and David Sheehan 20. Clinical Proteomics: Liquid Chromatography-Mass Spectrometry (LC/MS) Purification Systems Michael Henry and Paula Meleady 21. Strategies for the Purification of Membrane Proteins Sinead M. Smith 22. Antimicrobial Peptide Production and Purification Srinivas Suda, Des Field, and Niall Barron 23. Lectin Affiity Chromatography (LAC) < ` Brendan F. O'Connor, Donal Monaghan, and Jonathan Cawley

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詳細情報

• NII書誌ID(NCID): BB28315541
• ISBN:
  • 9781493964109
  • 9781493981915
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York
• ページ数/冊数: x, 426 p.
• 大きさ: 27 cm
• 分類:
  • DC23 : 572.6
  • DC20 : 574.192
• 件名:
  • LCSH : Proteins -- Analysis
  • LCSH : Chromatographic analysis
• 親書誌ID:
  • BA10794673
  • BA88873632