Western blotting : methods and protocols

著者

    • Kurien, Biji T.
    • Scofield, R. Hal

書誌事項

Western blotting : methods and protocols

edited by Biji T. Kurien, R. Hal Scofield

（Methods in molecular biology / John M. Walker, series editor, v. 1312）

Humana Press, c2015

  • : pbk

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

This volume covers past and present western blot techniques, such as diffusion blotting, slice blotting, blotting of high and low molecular weight proteins, single cell blotting and automated blotting. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Western Blotting: Methods and Protocols will serve as an invaluable reference for those interested in further study into this fascinating field.

目次

1. The Early Days of Blotting Edwin Southern 2. Origins of Protein Blotting Harry Towbin 3. Western Blotting: Remembrance of Things Past W. Neal Burnette 4. Simian Virus 40 and Protein Transfer Jaime Renart 5. Western Blotting: An Introduction Biji T. Kurien and R. Hal Scofield 6. From Little Helpers to Automation Harry Towbin 7. Spectrophotometric Methods to Determine Protein Concentration J.P. Dean Goldring 8. Solubilization of Proteins: The Importance of Lysis Buffer Choice Mandy Peach, Noelle Marsh, Ewa I. Miskiewicz, and Daniel J. MacPhee 9. Simultaneous Immunoblotting Analysis with Activity Gel Electrophoresis and 2-D Gel Electrophoresis Der-Yen Lee and Geen-Dong Chang 10. Diffusion Blotting: A Rapid and Simple Method for Production of Multiple Blots from a Single Gel Ingrid Olsen and Harald G. Wiker 11. Multiple Immunoblots by Passive Diffusion of Proteins from a Single SDS-PAGE Gel Biji T. Kurien and R. Hal Scofield 12. Slice Blotting Graeme Lowe 13. Localizing Proteins by Tissue Printing Rafael F. Pont-Lezica 14. Dot-Immunobinding Assay (Dot-Iba) Sumi Surendran, Annamma Mathai, and Vishnampet Venkataraman Radhakrishnan 15. Analysis of Antibody Clonotype by Affinity Immunoblotting Biji T. Kurien and R. Hal Scofield 16. Glycosaminoglycan Blotting and Detection after Electrophoresis Separation Nicola Volpi and Francesca Maccari 17. A Well-Based Reverse-Phase Protein Array of Formalin-Fixed Paraffin-Embedded Tissue Joon-Yong Chung and Stephen M. Hewitt 18. Quantitative Computerized Western Blotting in Detail Dalit Talmi-Frank, Charles L. Jaffe, and Gad Baneth 19. Cationic Electrophoresis and Eastern Blotting Engelbert Buxbaum 20. A Miniaturized Blotting System for Simultaneous Detecting of Different Autoantibodies Ulrich Canzler, Holger Bartsch, Kai Grossmann, Werner Lehmann, Karsten Conrad, Biji T. Kurien, Yaser Dorri, R. Hal Scofield, and Michael P. Bachmann 21. Proteomic Expressional Profiling of a Paraffin Embedded Tissue by Multiplex Tissue Immunoblotting Joon-Yong Chung and Stephen M. Hewitt 22. Post-Staining Electroblotting for Efficient and Reliable Peptide Blotting Der-Yen Lee and Geen-Dong Chang 23. Multistrip Western Blotting: A Tool for Comparative Quantitative Analysis of Multiple Proteins Edita Aksamitiene, Jan B. Hoek, and Anatoly Kiyatkin 24. Western Blotting using PVDF Membranes and Its Downstream Applications Setsuko Komatsu 25. Blotting from PhastGel to Membranes by Ultrasound Joseph Kost and Aharon Azagury 26. Western Blotting of High and Low Molecular Weight Proteins Using Heat Biji T. Kuien and R. Hal Scofield 27. Membrane Strip Affinity Purification of Autoantibodies Biji T. Kurien 28. Strip Immunoblotting of Multiple Antigenic Peptides Biji T. Kurien 29. Double-Blotting: A Solution to the Problem of Non-Specific Binding of Secondary Antibodies in Immunoblotting Procedures Francoise Lasne 30. Method for Resolution and Western Blotting of Very Large Proteins Using Agarose Electrophoresis Marion L. Greaser and Chad M. Warren 31. Immunodetection of P-Selectin Using an Antibody to its C-Terminal Tag Padmaja Mehta 32. Improvements and Variants of the Mutliple Antigen Blot Assay-MABA: An Immunoenzymatic Technique for Simultaneous Antigen and Antibody Screening Oscar Noya, Sandra Losada, Marilyan Toledo, Adriana Gauna, Maria Angelita Lorenzo, Henry Bermudez, and Belkisyole Alarcon de Noya 33. Blotting from Immobilized pH Gradient Gels: Application to Total Cell Lysates Harry Towbin 34. Immunoprecipitation: Western Blot for Proteins of Low Abundance Edward P. Trieu and Ira N. Targoff 35. Native Electrophoresis and Western Blot Analysis (NEWeB): Methods and Applications Ioannis N. Manoussopoulos and Mina Tsagris 36. Shift-Western Blotting: Separate Analysis of Protein and DNA from Protein-DNA Complexes Matthias Harbers 37. Grid-Immunoblotting Olga Yeliosof and R. Hal Scofield 38. Detection and Quantification of Protein-Protein Interactions by Far-Western Blotting Joshua A. Jadwin, Bruce J. Mayer, and Kazuya Machida 39. Western Blot Analysis of Adhesive Interactions under Fluid Shear Conditions: The Blot Rolling Assay Robert Sackstein and Robert Fuhlbrigge 40. Centrifuge Blotting Jinny Paul 41. Blotting of Coomassie Blue Stained Proteins from PAGE Gels to Transparencies Jinny Paul 42. B Cell ELISPOT: For the Identification of Antigen-Specific Antibody Secreting Cells Hemangi B. Shah and Kristi A. Koelsch 43. T Cell ELISPOT: For the Identification of Specific Cytokine-Secreting T Cells Kerry M. Leehan and Kristi A. Koelsch 44. Membrane Microplates for One- and Two-Color ELISPOT and FLUOROSPOT Assays Alexander E. Kalyuzhny 45. SDS PAGE to Immunoblot in One Hour Biji T. Kurien, Debashish Danda, Michael Bachmann, and R. Hal Scofield 46. Single Cell Western Blotting Syed MS Quadri 47. Protein Detection by Simple Western (TM) Analysis Valerie Harris 48. Western Blotting Using Microfluidics Pothur R. Srinivas 49. Two-Dimensional Gel-Based Protein Standardization Verified by Western Blot Analysis Hisao Haniu, Daisuke Watanabe, Yusuke Kawashima, and Hiroyuki Matsumoto 50. Fingerprint Deposition of Nitrocellulose and Polyvinylidene Difluoride Membranes Using Alkaline Phosphatase Biji T. Kurien, Debashish Danda, and R. Hal Scofield 51. Other Notable Protein Blotting Methods: A Brief Review Biji T. Kurien and R. Hal Scofield

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