Chemical and synthetic biology approaches to understand cellular functions

著者

• Shukla, Arun K.

書誌事項

Chemical and synthetic biology approaches to understand cellular functions

edited by Arun K. Shukla

（Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan, v. 621-622, 633）

Academic Press, 2019-2020

1st ed

• pt. A
• pt. B
• pt. C

注記

Includes bibliographical references

内容説明・目次

巻冊次

pt. A ISBN 9780128181171

内容説明

Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part A, Volume 621, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this volume covering Site-directed ethylation of membrane proteins for measuring conformational transitions in lipid bilayers, the Design and synthesis of fluorescent activity probes for protein phosphatases, Stains, Utilizing split-nanoLuc fragments as luminescent probes for protein solubility in living cells, SH2-domain based sensor for intracellular recognition of sulfo-tyrosine, DNA-encoded immunoglobulins for detection of parasites, An engineered TEV protease - calmodulin fusion based sensor for neuronal calcium recording, and much more.

目次

1. Targeted m6A reader proteins to study the epitranscriptome Simone Rauch and Bryan C. Dickinson 2. Synthetic gene expression circuits regulating sexual reproduction Nobuo Fukuda and Shinya Honda 3. Nucleotide resolution sequencing of N4-acetylcytidine in RNA Justin M. Thomas, Keri M. Bryson and Jordan L. Meier 4. A guide for drug inducible genome editing with HIT systems Chen Zhao, Shixian Wei and Yu Wang 5. A guide for drug inducible transcriptional activation with HIT systems Chen Zhao, Shixian Wei and Yu Wang 6. Direct cloning and heterologous expression of natural product biosynthetic gene clusters by transformation-associated recombination Jia Jia Zhang, Kazuya Yamanaka, Xiaoyu Tang and Bradley S. Moore 7. Salt-sensitive intein for large-scale polypeptide production Yi-Zong Lee and Shih-Che Sue 8. Methods for the recombinant expression of active tyrosine kinase domains: Guidelines and pitfalls M. Escarlet Diaz Galicia, Abdullah Aldehaiman, SeungBeom Hong, Stefan T. Arold and Raik Grunberg 9. Design, cloning and characterization of transcription factor-based inducible gene expression systems Erik K.R. Hanko, Nigel P. Minton and Naglis Malys 10. Design, construction, and validation of optogenetic proteins Colin P. O'Banion, Anwesha Goswami and David S. Lawrence 11. Overcoming component limitations in synthetic biology through transposon-mediated protein engineering Joshua T. Atkinson, Bingyan Wu, Laura Segatori and Jonathan J. Silberg 12. Chemical biology of glycoproteins: From chemical synthesis to biological impact Yaohao Li, Amy H. Tran, Samuel J. Danishefsky and Zhongping Tan 13. In bulla functional channel expression systems that mimic bacterial synthetic membranes Masayuki Iwamoto and Shigetoshi Oiki 14. A mass spectrometry-based isotope-coded mass tag method to map thiol accessibility in biological systems John E. Gadbery and Nicole S. Sampson 15. Quick-soaking of crystals reveals unprecedented insights into the catalytic mechanism of glycosyltransferases David Albesa-Jove, Javier O. Cifuente, Beatriz Trastoy and Marcelo E. Guerin 16. Cysteine-ethylation of tissue-extracted membrane proteins as a tool to detect conformational states by solid-state NMR spectroscopy Daniel K. Weber, Taysir Bader, Erik K. Larsen, Songlin Wang, Tata Gopinath, Mark Distefano and Gianluigi Veglia 17. Improved sensitivity and resolution of in-cell NMR spectra David S. Burz, Leonard Breindel and Alexander Shekhtman 18. High-throughput methods in aptamer discovery and analysis Kyle H. Cole and Andrej Luptak 19. Drop-in-well chamber for droplet interface bilayer with built-in electrodes Kazuhiro Urakubo, Masayuki Iwamoto and Shigetoshi Oiki

巻冊次

pt. B ISBN 9780128181195

内容説明

Chemical and Synthetic Biology Approaches To Understand Cellular Functions - Part B, Volume 622, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting chapters that cover the Design of optogenetic proteins, the Application of optogenetic proteins, Antibody aggregation mechanism probed by a fluorescently-labeled antibody with fluorescence correlation spectroscopy, Bimane labeling of B-arrestins to measure their interaction with GPCRs, Reversible biotinylation of proteins for investigating their interaction with partners, Chemical biology approaches to study RNA cytidine acetylation, Salt sensitive intein in robotic production of peptides, and much more.

目次

1. Input-dependent post-translational control of the reporter output enhances dynamic resolution of mammalian signaling systems Brianna E.K. Jayanthi, Wenting Zhao and Laura Segatori 2. Design and synthesis of fluorescent activity probes for protein phosphatases Garrett R. Casey, Jon R. Beck and Cliff I. Stains 3. Utilizing split-NanoLuc luciferase fragments as luminescent probes for protein solubility in living cells Travis J. Nelson, Jia Zhao and Cliff I. Stains 4. Engineering of a sulfotyrosine-recognizing small protein scaffold for the study of protein tyrosine O-sulfation Justin Lawrie, Wei Niu and Jiantao Guo 5. Synthesis and screening of bead-displayed combinatorial libraries Todd M. Doran, Paige Dickson, John Maina Ndungu, Peng Ge, Irena Suponitsky-Kroyter, Hongchan An and Thomas Kodadek 6. Visualizing the brain's astrocytes Alyssa N. Preston, Danielle A. Cervasio and Scott T. Laughlin 7. Modular activatable bioorthogonal reagents Pratik Kumar and Scott T. Laughlin 8. Engineering PEG-fatty acid stapled, long-acting peptide agonists for G protein-coupled receptors Sam Lear, Zaid Amso and Weijun Shen 9. Quantifying drug-target engagement in live cells using sulfonyl fluoride chemical probes Lyn H. Jones, Hua Xu and Olugbeminiyi O. Fadeyi 10. Selection and optimization of enzyme reporters for chemical cytometry Angela Proctor, Qunzhao Wang, David S. Lawrence and Nancy L. Allbritton 11. Synthetic cell-like membrane interfaces for probing dynamic protein-lipid interactions Spencer T. Glantz, Erin E. Berlew and Brian Y. Chow 12. Rational engineering acyltransferase domain of modular polyketide synthase for expanding substrate specificity Wan Zhang, Linjun Zhou, Chunyu Li, Zixin Deng and Xudong Qu 13. Identifying potentially O-GlcNAcylated proteins using metabolic labeling, bioorthogonal enrichment, and Western blotting Narek Darabedian and Matthew R. Pratt 14. Optogenetic perturbation of the biochemical pathways that control cell behavior Lauren L. Haar, David S. Lawrence and Robert M. Hughes 15. pH-shift stress on antibodies Hiroshi Imamura and Shinya Honda 16. Small molecule target identification using photo-affinity chromatography Seung-Yong Seo and Timothy W. Corson 17. Computational structural enzymology methodologies for the study and engineering of fatty acid synthases, polyketide synthases and nonribosomal peptide synthetases Andrew J. Schaub, Gabriel O. Moreno, Shiji Zhao, Hau V. Truong, Ray Luo and Shiou-Chuan Sheryl Tsai 18. On-cell coordination chemistry: Chemogenetic activation of membrane-bound glutamate receptors in living cells Ryou Kubota, Shigeki Kiyonaka and Itaru Hamachi 19. Bioorthogonal oncometabolite ligation Chloe A. Briney, Susana Najera and Jordan L. Meier

巻冊次

pt. C ISBN 9780128191286

内容説明

Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part C, Volume 633, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial. This release includes sections on Next generation probes for molecular imaging in cells, Competitive binding assay for biotin and biotin derivatives, based on avidin and biotin-4-fluorescein, Converting avidin to bind ligands other than biotin, especially steroids, Chemoenzymatic Labeling Strategy, Engineered Siderophores, Small molecules to inhibit bacterial population behavior, NMR tube bioreactor, Small molecule controlled RAS activation system, Small molecule regulated Cas9, the Design and application of synthetic receptors, and much more.

目次

1. Competitive binding assay for biotin and biotin derivatives, based on avidin and biotin-4-fluorescein Elke Oberbichler, Maria Wiesauer, Eva Schloegl, Jessica Stangl, Felix Faschinger, Gunther Knoer, Hermann J. Gruber and Vesa P. Hytoenen 2. (Strept)avidin as a template for ligands other than biotin: An overview Vesa P. Hytoenen 3. Engineering siderophores Sina Rutschlin and Thomas Boettcher 4. Competitive profiling for enzyme inhibitors using chemical probes Michaela Prothiwa and Thomas Boettcher 5. The NMR tube bioreactor Alexandra V. Chatzikonstantinou, Antonis Tsiailanis, Ioannis P. Gerothanassis, Haralambos Stamatis, Enrico Ravera, Marco Fragai, Claudio Luchinat, Giacomo Parigi and Andreas G. Tzakos 6. A chemically-controlled system for activating RAS GTPases Emily M. Dieter and Dustin J. Maly 7. Temporal and rheostatic control of genome editing with a chemically-inducible Cas9 Cindy T. Wei, Dustin J. Maly and Douglas M. Fowler 8. Synthetic receptors to understand and control cellular functions Hung-Ju Chang and Jerome Bonnet 9. A suite of bioassays to evaluate CREB inhibitors Bingbing X. Li and Xiangshu Xiao 10. Identification of lamins as the molecular targets of LBL1 using a clickable photoaffinity probe Xiangshu Xiao and Bingbing X. Li 11. REX technologies for profiling and decoding the electrophile signaling axes mediated by Rosetta Stone proteins Marcus J. C. Long, Daniel A. Urul and Yimon Aye 12. Building artificial genetic circuits to understand protein function Louis H. Scott, James C. Mathews, Aleksandra Filipovska and Oliver Rackham 13. Methods for studying human sirtuins with activity-based chemical probes Song Zheng, Jessica Wohlfahrt, Ian Cohen and Yana Cen 14. Site-directed labeling of ss-arrestin with monobromobimane for measuring their interaction with G protein-coupled receptors Ashish Srivastava, Mithu Baidya, Hemlata Dwivedi-Agnihotri and Arun K. Shukla 15. Reversible biotinylation of purified proteins for measuring protein-protein interactions Hemlata Dwivedi-Agnihotri, Ashish Srivastava and Arun K. Shukla

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詳細情報

• NII書誌ID(NCID): BB28360819
• ISBN:
  • 9780128181171
  • 9780128181195
  • 9780128191286
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: Cambridge, Mass.
• ページ数/冊数: 3 v.
• 大きさ: 24 cm
• 分類:
  • LCC : QH581.2
  • DC23 : 571.6
• 件名:
  • LCSH : Cytology
  • LCSH : Cell physiology
  • LCSH : Enzymology
• 親書誌ID:
  • BA00197543