Microcalorimetry of biological molecules : methods and protocols

著者

    • Ennifar, Eric

書誌事項

Microcalorimetry of biological molecules : methods and protocols

edited by Eric Ennifar

（Methods in molecular biology / John M. Walker, series editor, 1964）（Springer protocols）

Humana Press, c2019

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注記

Includes index

内容説明・目次

内容説明

This volume provides methods on microcalorimetry approaches to investigate complex biological molecular systems. Chapters guide readers through Differential Scanning Calorimetry (DSC), Isothermal Titration Calorimetry (ITC), and advanced data processing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microcalorimetry of Biological Molecules: Methods and Protocols aims to ensure successful results in the further study of this vital field.

目次

Preface... Table of Contents... Contributing Authors... Part I Differential Scanning Calorimetry 1. The Contribution of Differential Scanning Calorimetry for the Study of Peptide/Lipid Interactions Marie-Lise Jobin and Isabel D. Alves 2. Protocols of IATC, DSC, and PPC: The Multi-State Structural Transition of Cytochrome c Shigeyoshi Nakamura and Shun-ichi Kidokoro 3. Value of DSC in Characterization and Optimization of Protein Stability Katherine Bowers and Natalia Markova 4. Plasmatic Signature of Disease by Differential Scanning Calorimetry (DSC) Philipp O. Tsvetkov and Francois Devred Part II Isothermal Titration Calorimetry 5. Intrinsic Thermodynamics of Protein - Ligand Binding by Isothermal Titration Calorimetry as Aid to Drug Design Vaida Paketuryte, Asta Zubriene, John E. Ladbury, and Daumantas Matulis 6. Isothermal Titration Calorimetry Measurements of Riboswitch-Ligand Interactions Christopher P. Jones, Grzegorz Piszczek, and Adrian Ferre-D'Amare 7. ITC Studies of Ribosome/Antibiotics Interactions Emma Schenckbecher, Benoit Meyer, and Eric Ennifar 8. High Quality Data of Protein/Peptide Interaction by Isothermal Titration Calorimetry Juan Ramirez and Yves Nomine 9. ITC Measurement for High Affinity Aptamers Binding to their Target Proteins Ryo Amano, Tomohisa Furukawa, and Taiichi Sakamoto 10. Thermodynamics of Molecular Machines using Incremental ITC Benoit Meyer, Cyrielle da Veiga, Philippe Dumas, and Eric Ennifar 11. Measuring the Metabolic Activity of Mature Mycobacteria Biofilms using Isothermal Microcalorimetry Anna Solokhina, Gernot Bonkat, and Olivier Braissant 12. Characterization of Microtubule-Associated Proteins (MAPs) and Tubulin Interactions by Isothermal Titration Calorimetry (ITC) Philipp O. Tsvetkov, Romain La Rocca, Soazig Malesinski, and Francois Devred Part III Advanced Data Processing and Kinetics 13. Analysis of Isothermal Titration Calorimetry Data for Complex Interactions using I2CITC Ibrahim Q. Saeed and Niklaas Buurma 14. Tinkering with Binding Polynomials in Isothermal Titration Calorimetry Rafael Claveria-Gimeno, Sonia Vega, Olga Abian, and Adrian Velazquez-Campoy 15. Use of ITC and the Software AFFINImeter for the Quantification of Anticoagulant Pentasaccharide in Low Molecular Weight Heparine Eva Munoz and Guillermo Franco 16. Thermodynamic and Kinetic Analysis of Isothermal Titration Calorimetry Experiments by using kinITC in AFFINImeter Eva Munoz, Juan Sabin, Javier Rial, Daniel Perez, Eric Ennifar, Philippe Dumas, and Angel Pineiro 17. Enzyme Kinetics Determined by Single-Injection Isothermal Titration Calorimetry Colette F. Quinn and Lee D. Hansen 18. Characterization of Enzymatic Reactions using ITC Barbara Zambelli

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