Radiation cytogenetics : methods and protocols
著者
書誌事項
Radiation cytogenetics : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1984）（Springer protocols）
Humana Press, c2019
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This book comprises a collection of chapters describing topics from traditional radiation cytogenetic analysis methods to the modern fluorescence based-analysis and high throughput automatic analysis methods. Cytogenetic analysis is important not only in order to understand radiation-induced DNA damage in cells, but it is also a standard biological method to estimate human radiation exposure in the radiation accidents. Therefore, we prepared a series of chapters not only covering biological aspects but also exploring radiation physics and radiation protection. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and equipment, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Radiation Cytogenetics: Methods and Protocols serves both novices working on their first experiments as well as experienced researchers searching for tried-and-true techniques to adapt for their own laboratory.
目次
1. Human Lymphocyte Metaphase Chromosome Preparation for Radiation-Induced Chromosome Aberration Analysis
Takamitsu A. Kato
2. Animal Lymphocyte Metaphase Chromosome Preparation
Kazuki Heishima, Kendon Kuo, Masashi Kimura, and Takashi Mori
3. Micronuclei Formation Analysis after Ionizing Radiation
Cathy Su, Alexis H. Haskins, and Takamitsu A. Kato
4. G1 Premature Chromosome Condensation (PCC) Assay
Ryuichi Okayasu and Cuihua Liu
5. G2 Chromosomal Radiosensitivity Assay for Testing Individual Radiation Sensitivity
Jeremy S. Haskins and Takamitsu A. Kato
6. G2 Premature Chromosome Condensation/Chromosome Aberrations Assay: Drug-Induced Premature Chromosome Condensation (PCC) Protocols and Cytogenetic Approaches in Mitotic Chromosome and Interphase Chromatin for Radiation Biology
Eisuke Gotoh
7. Sister Chromatid Exchange as a Genotoxic Stress Marker
Shigeaki Sunada, Jeremy S. Haskins, and Takamitsu A. Kato
8. DNA Damage Focus Formation Assay
Yoshihiro Fujii
9. Nuclear Foci Assays in Live Cells
Eiichiro Mori and Aroumougame Asaithamby
10. In Situ DNA Damaging Foci Analysis on Metaphase Chromosomes
Chisato Omata and Takamitsu A. Kato
11. PNA Telomere and Centromere FISH Staining for Accurate Analysis of Radiation-Induced Chromosomal Aberrations
Ian M. Cartwright, Jeremy S. Haskins, and Takamitsu A. Kato
12. Modified PNA Telomere and Centromere FISH Protocols
Ian M. Cartwright
13. Directional Genomic Hybridization (DGH) for Detection of Intra-Chromosomal Rearrangements
Erin Robinson, Miles J. McKenna, Joel S. Bedford, Edwin H. Goodwin, Michael N. Cornforth, Susan M. Bailey, and F. Andrew Ray
14. Reciprocal Translocation Analysis with Whole Chromosome Painting for FISH
Jeremy S. Haskins and Takamitsu A. Kato
15. Analysis of Radiation-Induced Chromosome Exchanges Using Combinatorial Chromosome Painting
Bradford D. Loucas
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