Methods in kidney cell biology
著者
書誌事項
Methods in kidney cell biology
（Methods in cell biology / edited by David M. Prescott, v. 153-154）
Academic Press, an imprint of Elsevier, 2019
- pt. A
- pt. B
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注記
Includes bibliographical references
内容説明・目次
- 巻冊次
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pt. A ISBN 9780128170823
内容説明
Methods in Kidney Cell Biology, Volume 153, represents state-of-the-art techniques in renal research that are ideal for veterans, graduate students, postdoctoral fellows, and clinical scientists and principal investigators. Topics in the new release include Single glomerular proteomics - a novel method in translational glomerular cell biology, Measurement of cytosolic and intraciliary calcium in live cells, Differentiation of human kidney organoids from pluripotent stem cells, Quantifying autophagic flux in kidney tissue using structured illumination microscopy, the Generation of primary cells from ADPKD and normal human kidneys, ADPKD cell proliferation and Cl-dependent fluid secretion, In vitro cyst formation of ADPKD cells, and much more.
目次
1. Generation of primary cells from ADPKD and normal human kidneys Darren P. Wallace and Gail A. Reif 2. Measurement of cytoplasmic and cilioplasmic calcium in a single living cell Rinzhin T. Sherpa, Rajasekharreddy Pala, Ashraf M. Mohieldin and Surya M. Nauli 3. Application of physiological shear stress to renal tubular epithelial cells Nicholas Ferrell, Ruben M. Sandoval, Bruce A. Molitoris, Paul Brakeman, Shuvo Roy and William H. Fissell 4. ADPKD cell proliferation and Cl--dependent fluid secretion Gail A. Reif and Darren P. Wallace 5. In vitro cyst formation of ADPKD cells Madhulika Sharma, Gail A. Reif and Darren P. Wallace 6. Engineering kidney tissues for polycystic kidney disease modeling and drug discovery Valerio Brizi, Valentina Benedetti, Angelo Michele Lavecchia and Christodoulos Xinaris 7. Differentiation of human kidney organoids from pluripotent stem cells Nelly M. Cruz and Benjamin S. Freedman 8. Studying Na+ and K+ channels in aldosterone-sensitive distal nephrons Jacques Teulon and Wen-Hui Wang 9. Metanephric organ culture Robin L. Maser, Brenda S. Magenheimer and James P. Calvet 10. Ex vivo kidney slice preparations as a model system to study signaling cascades in kidney epithelial cells Biagio Saitta, Michael F. Jalili, Hamidreza Zohoorkari, Renee Rao, Kenneth R. Hallows, Catherine J. Baty and Nuria M. Pastor-Soler 11. Analysis of primary cilia in renal tissue and cells Luciane M. Silva, Wei Wang, Bailey A. Allard, Tana S. Pottorf, Damon T. Jacobs and Pamela V. Tran 12. Quantifying autophagic flux in kidney tissue using structured illumination microscopy Kensei Taguchi, Bertha C. Elias, Subo Qian and Craig R. Brooks 13. Investigation of epigenetics in kidney cell biology Linda Xiaoyan Li, Ewud Agborbesong, Lu Zhang and Xiaogang Li
- 巻冊次
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pt. B ISBN 9780128203354
内容説明
Methods in Kidney Cell Biology, Part B, Volume 154 represents state-of-the-art techniques in renal research that are ideal for veterans, graduate students, postdoctoral fellows, clinical scientists and principal investigators. Topics in the new release include Single glomerular proteomics - a novel method in translational glomerular cell biology, Measurement of cytosolic and intraciliary calcium in live cells, Differentiation of human kidney organoids from pluripotent stem cells, Quantifying autophagic flux in kidney tissue using structured illumination microscopy, the Generation of primary cells from ADPKD and normal human kidneys, ADPKD cell proliferation and Cl-dependent fluid secretion, In vitro cyst formation of ADPKD cells, and much more.
目次
1. Single glomerular proteomics - a novel tool for translational glomerular cell biology Markus Rinschen 2. Kidney harvesting and metabolite extraction for metabolomics studies in rodents Maria Irazabal Mira 3. Optical tissue clearing and immunolabeling in kidney research David Ellison 4. Urinary extracellular vesicles as a source of biomarkers reflecting renal cellular biology in human disease Roman Muller 5. Intravital Visualization of the Primary Cilium, Tubule Flow, and Innate Immune Cells in the Kidney Utilizing an Abdominal Window Imaging Approach Bradley Yoder 6. Novel fluorescence techniques to quantitate renal cell biology Janos Peti-Peterdi 7. Manipulation of Renal Gene Expression Using Oligonucleotides Vishal Patel 8. Methods for renal lineage tracing: in vivo and beyond Thomas Carroll 9. In Vivo Magnetic Resonance Imaging Techniques for Structural and Functional Characterization of Murine Model Kidneys Tim Kline 10. In vivo analysis of renal epithelial cells in zebrafish Zhaoxia Sun 11. Visualizing gene expression during zebrafish pronephros development and regeneration Rebecca Wingert 12. Drosophila melanogaster and its nephrocytes - a versatile model for glomerular research Paul Brinkkotter
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