Microbial metabolomics : methods and protocols
著者
書誌事項
Microbial metabolomics : methods and protocols
（Methods in molecular biology / John M. Walker, series editor）
Humana Press, c2019
大学図書館所蔵 全1件
注記
Includes bibliographical references and index
内容説明・目次
内容説明
This detailed volume includes protocols that represent the breadth of microbial metabolomics approaches to both large-scale and small-scale experiments with intention of highlighting techniques that can be used for applications ranging from environmental microbiology to human disease. Utilizing mass spectrometry as their primary measurement tool, the chapters explore microbial metabolomics, metabolism and microbial physiology, metabolite sample preparation, current analytical techniques used to profile primary and secondary metabolites and lipids, as well as establishing data analysis workflows for targeted metabolomics, untargeted metabolomics, analysis of metabolic fluxes, and genome-scale models. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Microbial Metabolomics: Methods and Protocols serves as an ideal reference for both novice and advanced users and can be adapted to similar analytical platforms or customized to suit the needs of the researcher.
目次
1. Microbial Metabolomics: A General Overview
Edward E.K. Baidoo
Part I: Microbial Metabolomics, Metabolism, and Physiology
2. Mass Spectrometry-Based Microbial Metabolomics: Techniques, Analysis, and Applications
Edward E.K. Baidoo and Veronica Teixeira Benites
3. Metabolomics: A Microbial Physiology and Metabolism Perspective
Chijioke J. Joshua
Part II: Metabolite Sample Preparation
4. Untargeted Soil Metabolomics Using Liquid Chromatography-Mass Spectrometry and Gas Chromatography-Mass Spectrometry
Tami L. Swenson and Trent R. Northen
5. Fatty Acid Metabolome Extraction from Mycobacterial Cells for GC-MS Metabolomics Analysis
Ilse du Preez, Derylize Beukes, and Du Toit Loots
6. Total Metabolome Extraction from Mycobacterial Cells for GC-MS Metabolomics Analysis
Derylize Beukes, Ilse du Preez, and Du Toit Loots
7. High-Throughput Solid Phase Microextraction-Liquid Chromatography-Mass Spectrometry for Microbial Untargeted Metabolomics
Fatemeh Mousavi, Barbara Bojko, and Janusz Pawliszyn
Part III: Current Analytical Techniques
8. Targeted Metabolomics of Xylose Fermenting Yeasts Based on Mass Spectrometry
Christiane Goncalves Campos, Jose Antonio de Aquino Ribeiro, Joao Ricardo Moreira de Almeida, Betania Ferraz Quirino, and Patricia Verardi Abdelnur
9. Exploiting High-Resolution Mass Spectrometry for Targeted Metabolite Quantification and 13C-labeling Metabolism Analysis
Zhucui Li, Yujing Li, Yinjie J. Tang, and Wenqing Shui
10. Quantitative Profiling of Endogenous Metabolites Using Hydrophilic Inter-Action Liquid Chromatography-Tandem Mass Spectrometry (HILIC-MS/MS)
Attila Teleki and Ralf Takors
11. Liquid Chromatography and Mass Spectrometry Analysis of Isoprenoid Intermediates in Escherichia coli
Edward E.K. Baidoo, George Wang, Chijioke Joshua, Veronica Teixeira Benites, and Jay D. Keasling
12. Determining the Mode of Action of Antimalarial Drugs Using Time-Resolved LC-MS-Based Metabolite Profiling
Simon A. Cobbold and Malcolm J. McConville
13. Use of Liquid Chromatography-Mass Spectrometry-Based Metabolomics to Identify Biomarkers of Tuberculosis
Juntuo Zhou and Yuxin Yin
14. Metabolomics Analysis of Leishmania by Capillary Electrophoresis and Mass Spectrometry
David Rojo, Coral Barbas, and Angeles Lopez-Gonzalvez
Part IV: Data Analysis
15. A High-Throughput Targeted Metabolomics Workflow for the Detection of 200 Polar Metabolites in Central Carbon Metabolism
Yuping Cai and Zheng-Jiang Zhu
16. Cluster Analysis of Untargeted Metabolomic Experiments
Joshua Heinemann
17. Machine Learning in Untargeted Metabolomics Experiments
Joshua Heinemann
18. Dynamic 13C Labeling of Fast Turnover Metabolites for Analysis of Metabolic Fluxes and Metabolite Channeling
Mary Abernathy, Ni Wan, Wenqing Shui, and Yinjie J. Tang
19. Genome-Scale 13C Fluxomics Modeling for Metabolic Engineering of Saccharomyces cerevisiae
David Ando and Hector Garcia Martin
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