Electroporation protocols : microorganism, mammalian system, and nanodevice
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Bibliographic Information
Electroporation protocols : microorganism, mammalian system, and nanodevice
（Methods in molecular biology / John M. Walker, series editor, 2050）（Springer protocols）
Humana Press, c2020
3rd ed
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Includes bibliographical references and index
Description and Table of Contents
Description
This third edition provides in-depth knowledge on the delivery of naked DNA and small-interfering RNA (siRNA) to the targeted microorganism, mammalian single cells, tissues, and animals for prevention and treatment of disease. It builds on the success of the first edition and on the progress made in siRNA delivery and DNA vaccines for large animals as well as discovery of electroporation applications for the fragile tissues and for internal organs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Electroporation Protocols: Microorganism, Mammalian System, and Nanodevice, Third Edition aims to be an invaluable resource for investigators both in and outside of this field.
Table of Contents
Part I: Design and Fabrication of Micro-/Nano-Electroporation
1. Cell Size-specific Transfection by Micropillar Array Electroporation
Xuan Liu, Yingbo Zu, and Shengnian Wang
2. Soft Electroporation through 3D Hollow Nanoelectrodes
Jun Yin and Yang Li
3. Micromachining of Polymeric Microfluidic Micro-/Nano-Electroporation Device
Lei Li
4. 3D Nanochannel Array for High-Throughput Cell Manipulation and Electroporation
Lingqian Chang, Stephen Black, Chandani Chitrakar, and Mehdi Nouri
5. Low-Voltage Flow-Through Electroporation Membrane and Method
Juliette Experton, Aaron G. Wilson, and Charles R. Martin
Section II: Micro-/Nano-electroporation for in vitro Delivery
6. Nanofountain Probe Electroporation for Monoclonal Cell Line Generation
Horacio D. Espinosa, Prithvijit Mukherjee, and Cesar Patino
7. 3D Nanochannel Electroporation for Macromolecular Nucleotides Delivery
Lingqian Chang, Chandani Chitrakar, and Mehdi Nouri
8. Nanoelectroporation and Collection of Genetically Modified Exosomes in Primary Cultures of Dendritic Cells
Silvia Duarte-Sanmiguel, Natalia Higuita-Castro, and Daniel Gallego-Perez
9. Electroporation of CRISPR-Cas9 into Malignant B Cells for Loss-of-function Studies of Target Gene via Knockout
Jian Yu
10. Microfluidic Device for Localized Electroporation
Justin Brooks and Ruiguo Yang
Part III: Advanced in vivo Electroporation
11. Transdermal Delivery of Nucleic Acid Mediated by Punching and Electroporation
Dong Huang, Yuanyu Huang, and Zhihong Li
12. Targeted in vivo Electroporation Using Nanoengineered Microelectrodes
Daniel Schwarz and Andreas T. Schaefer
13. Zygote Electroporation for CRISPR/Cas9 Delivery to Generate Genetically Modified Mice
Tatsuya Takemoto
14. Genome Editing in Staphylococcus aureus by Conditional Recombineering and CRISPR/Cas9-mediated Counterselection
Kelsi Penewit and Stephen J. Salipante
15. Tissue Nano-Transfection of Surgically Exposed Stroma
Natalia Higuita-Castro, Jordan Moore, Ludmila Diaz-Starokozheva, Cameron Rink, Ian Valerio, and Daniel Gallego-Perez
Section IV: Microorganism Electroporation
16. Transformation of a Model Microalga, Chlamydomonas reinhardtii without Cell-wall Removal
Takashi Yamano and Hideya Fukuzawa
17. Electroporation Transformation Protocol for Phaeodactylum tricornutum
Hanhua Hu and Yufang Pan
18. Efficient Transformation of the Diatoms Phaeodactylum tricornutum by Multi-pulse Electroporation
Kentaro Ifuku and Dongyi Yan
19. Rapid and Effective Electroporation Protocol for Nannochloropsis oceanica
Da-Wei Li, Srinivasan Balamurugan, Jian-Wei Zheng, Wei-Dong Yang, Jie-Sheng Liu, and Hong-Ye Li
20. Electroporation Procedures for Genetic Modification of Green Algae Chlorella spp.
Liang Ji and Jianhua Fan
21. Electrotransformation of Saccharomyces cerevisiae
Meilhoc E and Teissie J
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