Plant epigenetics : methods and protocols
著者
書誌事項
Plant epigenetics : methods and protocols
（Springer protocols）（Methods in molecular biology / John M. Walker, series editor, 1456）
Humana Press, c2017
2nd ed
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This
volume provides a variety of protocols to analyze various epigenetic
changes, including differential expression of non-coding RNAs, changes in DNA
methylation, and histone modifications in plants. Chapters detail protocols
with different degrees of complexity, and describe bioinformatics approaches
for data processing and analysis. Written in the highly successful Methods
in Molecular Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents, step-by-step,
readily reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls.
Authoritative and
cutting-edge, Plant Epigenetics: Methods and Protocols, Second Edition
aims to ensure successful results in the further study of this vital field.
目次
1. Chromatin Immunoprecipitation
Protocol for Histone Modifications and Protein-DNA Binding Analyses in Arabidopsis
Wanhui You, Stephane Pien, and Ueli
Grossniklaus
2.
Chromatin Conformation Capture-Based Analysis of Nuclear Architecture
Stefan Grob and Ueli Grossniklaus
3.
Metaanalysis of Genome-Wide Chromatin Data
Julia
Engelhorn and Franziska Turck
4.
Localization of Mirnas by In Situ Hybridization in Plants using Conventional
Oligonucleotide Probes
Sara
Hernandez-Castellano, Geovanny I. Nic-Can, Clelia
De-La-Pena
5.
COBRA (Combined Bisulfite Restriction Analysis) Assay for the Analysis of
Locus-Specific Changes in Methylation Patterns
Andriy
Bilichak and Igor Kovalchuk
6.
Analysis of Global Genome Methylation Using the Cytosine-Extension Assay
Andriy
Bilichak and Igor Kovalchuk
7.
In Situ Analysis Of DNA Methylation in Plants
Palak
Kathiria and Igor Kovalchuk
< 8. Analysis of DNA Hydroxymethylation in Arabidopsis Using
Colorimetric Assay
Andrey Golubov and Igor Kovalchuk
9. Analysis of DNA Cytosine Methylation Patterns using
Methylation Specific Amplification Polymorphism (MSAP)
Maria Angeles Guevara, Nuria De Maria, Enrique Saez-Laguna,
Maria Dolores Velez, Maria Teresa Cervera, and Jose Antonio Cabezas
10. Differentially Methylated
Region - Representational Difference Analysis (DMR-RDA): A Powerful Method to Identify
Dmrs in Uncharacterized Genomes
Pavlina Sasheva Ueli Grossniklaus
11. Analysis of Small RNA Populations Using
Hybridization to DNA Tiling Arrays
Martine
Boccara, Alexis Sarazin, Bernard Billoud, Agnes Bulski, Louise Chapell, David
Baulcombe, and Vincent Colot
12. Northern Blotting Techniques for Small RNAs
Todd
Blevins
13. Stem-Loop QRT-PCR for the Detection of Plant
Micrornas
Erika Varkonyi-Gasic
14.
Profiling New Small RNA Sequences
Masayuki Tsuzuki and Yuichiro Watanabe
15.
Small RNA Library Preparation and Illumina Sequencing in Plants
Andriy
Bilichak, Andrey Golubov, and Igor Kovalchuk
16.
Bioinformatics Analysis of Small RNA Transcriptomes - The Detailed Work Flow<
Slava
Ilnytskyy and Andriy Bilichak
17. Increasing
a Stable Transformation Efficiency of Arabidopsis by Manipulating the
Endogenous Gene Expression using Virus-Induced Gene Silencing
Andriy
Bilichak and Igor Kovalchuk
18.
The Random Oligonucleotide-Primed Synthesis Assay for the Quantification of DNA
Strand Breaks
Andriy
Bilichak and Igor Kovalchuk
19.
Profiling Transposable Elements and their Epigenetic Effects in Non-Model
Species
Christian
Parisod
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