Enzyme activity in single cells

著者

    • Allbritton, Nancy L.
    • Kovarik, Michelle L.

書誌事項

Enzyme activity in single cells

edited by Nancy L. Allbritton, Michelle L. Kovarik

(Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan, v. 628)

Academic Press, 2019

1st ed

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注記

Includes bibliographical references

内容説明・目次

内容説明

Enzyme Activity in Single Cells, Volume 628, the latest release in the Methods of Enzymology series, discusses groundbreaking cellular physiology research that is taking place in the biological sciences. Chapters in this new release cover Spatial and temporal resolution of caspase waves in single Xenopus eggs during apoptosis, Spatial and temporal organization of metabolic complexes in cells, Measuring cellular efflux and biomolecular delivery: synthetic approaches to imaging and engineering cells, Slide-based, single-cell enzyme assays, Single-cell assays using integrated continuous-flow microfluidics, High-throughput screening of single-cell lysates, Microfluidic capture of single cells for drug resistance assays, and much more.

目次

1. Phase-separated condensates of metabolic complexes in living cells: Purinosome and glucosome Songon An, Miji Jeon, Erin L. Kennedy and Minjoung Kyoung 2. Synthetic probe development for measuring single or few-cell activity and efflux Alison Lui, Jeffrey Wang, Linda Chio and Markita P. Landry 3. Enzymatic activity in single cells Josephine Geertsen Keller, Magnus Stougaard and Birgitta R. Knudsen 4. Single-cell assays using integrated continuous-flow microfluidics Ee Xien Ng, Myat Noe Hsu, Guoyun Sun and Chia-Hung Chen 5. Single-cell activity screening in microfluidic droplets Stefanie Neun, Tomasz S. Kaminski and Florian Hollfelder 6. The microfluidic capture of single breast cancer cells for multi-drug resistance assays Karan Parekh, Hamide Sharifi, Avid Khamenehfar, Timothy V. Beischlag, Robert T.M. Payer and Paul C.H. Li 7. Single-cell proteolytic activity measurement using microfluidics for rare cell populations Yu-Chih Chen and Euisik Yoon 8. Microfluidic technology for investigation of protein function in single adherent cells Aldo Jesorka, Inga Poldsalu and Irep Goezen 9. Nanokits for the electrochemical quantification of enzyme activity in single living cells Rongrong Pan and Dechen Jiang 10. Design of an automated capillary electrophoresis platform for single-cell analysis David H. Abraham, Matthew M. Anttila, Luke A. Gallion, Brae V. Petersen, Angela Proctor and Nancy L. Allbritton 11. Microelectrophoretic single-cell measurements with microfluidic devices Jay Sibbitts, Jalal Sadeghi and Christopher T. Culbertson 12. Chemical probes for spatially resolved measurement of active enzymes in single cells Gang Li and Raymond E. Moellering 13. Single-cell proteomics in complex tissues using microprobe capillary electrophoresis mass spectrometry Camille Lombard-Banek, Sam B. Choi and Peter Nemes 14. Electroosmotic extraction coupled to mass spectrometry analysis of metabolites in live cells Ruichuan Yin, Venkateshkumar Prabhakaran and Julia Laskin

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関連文献: 1件中  1-1を表示

  • Methods in enzymology

    editors in chief, Sidney P. Colowick, Nathan O. Kaplan

    Academic Press c1955-

    v. 1 , v. 2 , v. 3 , v. 4 , v. 5 , v. 6 , v. 7 , v. 33 , v. 75 , v. 95 , v. 120 , v. 140 , v. 175 , v. 199 , v. 229 , v. 265 , v. 285 , v. 320 , v. 355

    所蔵館179館

詳細情報

  • NII書誌ID(NCID)
    BB29176881
  • ISBN
    • 9780128170908
  • 出版国コード
    us
  • タイトル言語コード
    eng
  • 本文言語コード
    eng
  • 出版地
    Cambridge, Mass.
  • ページ数/冊数
    xvii, 307 p.
  • 大きさ
    24 cm
  • 分類
  • 件名
  • 親書誌ID
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