Mouse cell culture : methods and protocols
Author(s)
Bibliographic Information
Mouse cell culture : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1940）（Springer protocols）
Humana Press, c2019
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Note
Includes bibliographical references and index
Description and Table of Contents
Description
This volume describes cell culture protocols for the maintenance, propagation, manipulation, and analysis of primary explanted cells from various mouse organ systems. After introductory chapters that examine the applicability of mouse models as a discovery tool and describe critical factors and variables that influence cell culture endpoints, protocol chapters explore cell culture methodologies for specific mouse cell types and lineages. Written in the highly successful Methods in Molecular Biology series format, these chapters include introductions to their respective topics, comprehensive lists of the essential materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Mouse Cell Culture: Methods and Protocols aims to ensure successful results utilizing these protocols in further studies, leading to the identification of key factors, genes, and cellular pathways regulating cell lineage commitment and differentiation, modeling disease, and identifying novel therapeutic targets.
Table of Contents
Part I: Practical Considerations
1. The Applicability of Mouse Models to the Study of Human Disease
Kristina Rydell-Toermanen and Jill R. Johnson
2. Optimizing the Cell Culture Microenvironment
Ivan Bertoncello
Part II: Methods and Protocols
3. Propagation and Maintenance of Mouse Embryonic Stem Cells
Jacob M. Paynter, Joseph Chen, Xiaodong Liu, and Christian M. Nefzger
4. Production of High Titer Lentiviral Particles for Stable Genetic Modification of Mammalian Cells
Michael R. Larcombe, Jan Manent, Joseph Chen, Ketan Mishra, Xiaodong Liu, and Christian M. Nefzger
5. Generation of Mouse Induced Pluripotent Stem Cells by Lentiviral Transduction
Xiaodong Liu, Joseph Chen, Jaber Firas, Jacob M. Paynter, Christian M. Nefzger, and Jose M. Polo
6. Gene Editing of Mouse Embryonic and Epiblast Stem Cells
Tennille Sibbritt, Pierre Osteil, Xiaochen Fan, Jane Sun, Nazmus Salehin, Hilary Knowles, Joanne Shen, and Patrick P.L. Tam
7. Identification of Circulating Endothelial Colony Forming Cells from Murine Embryonic Peripheral Blood
Yang Lin, Chang-Hyun Gil, and Mervin C. Yoder
8. Imaging and Analysis of Mouse Embryonic Whole Lung, Isolated Tissue, and Lineage-Labeled Cell Culture
Matthew Jones and Saverio Bellusci
9. Mouse Hematopoietic Stem Cell Modification and Labeling by Transduction and Tracking Post Transplantation
Benjamin Cao, Songhui Li, Claire Pritchard, Brenda Williams, and Susan K. Nilsson
10. Genetic Manipulation and Selection of Mouse Mesenchymal Stem Cells for Delivery of Therapeutic Factors In Vivo
Donald S. Sakaguchi
11. Isolation and Culture of Primary Mouse Middle Ear Epithelial Cells
Apoorva Mulay, Khondoker Akram, Lynne Bingle, and Colin D. Bingle
12. Isolation and Propagation of Lacrimal Gland Putative Epithelial Progenitor Cells
Helen P. Makarenkova and Robyn Meech
13. Organotypic Culture of Adult Mouse Retina
Brigitte Muller
14. Langendorff-Free Isolation and Propagation of Adult Mouse Cardiomyocytes
Matthew Ackers-Johnson and Roger S. Foo
15. Isolation, Culture, and Characterization of Primary Mouse Epidermal Keratinocytes
Ling-Juan Zhang
16. Isolation and Propagation of Mammary Epithelial Stem and Progenitor Cells
Julie M. Sheridan and Jane E. Visvader
17. An Organoid Assay for Long-Term Maintenance and Propagation of
Mouse Prostate Luminal Epithelial Progenitors and Cancer Cells
Yu Shu and Chee Wai Chua
18. Isolation, Purification, and Culture of Mouse Pancreatic Islets of Langerhans
Youakim Saliba and Nassim Fares
19. Identification and In Vitro Expansion of Adult Hepatocyte Progenitors from Chronically Injured Livers
Naoki Tanimizu
20. The Preparation of Decellularized Mouse Lung Matrix Scaffolds for Analysis of Lung Regenerative Cell Potential
Deniz A. Boelukbas, Martina M. De Santis, Hani N. Alsafadi, Ali Doryab, and Darcy E. Wagner
21. Mouse Lung Tissue Slice Culture
Xinhui Wu, Eline M. van Dijk, I. Sophie T. Bos, Loes E.M. Kistemaker, and Reinoud Gosens
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