Plant chemical genomics : methods and protocols

著者

• Fauser, Friedrich
• Jonikas, Martin

書誌事項

Plant chemical genomics : methods and protocols

edited by Friedrich Fauser and Martin Jonikas

（Methods in molecular biology / John M. Walker, series editor）（Springer protocols）

Humana press, c2018

注記

Includes bibliographical references and index

内容説明・目次

内容説明

This volume discusses easy-to-follow protocols for researchers in the field of plant chemical genomics. The chapters in this book look at technical workflows of high-throughput chemical compound screens in numerous plant tissues, such as seedlings, roots, and pollen. Chapters also focus on single-celled systems such as Chlamydomonas reinhardtii for chemical screens, and techniques used to modify the target of a small molecule. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Plant Chemical Genomics: Methods and Protocols is a valuable resource to help researchers work with a broad variety of plants for chemical genomics approaches.

目次

Preface... Table of Contents... Contributing Authors... 1. Multi-Parametric Screening in Arabidopsis thaliana Seedlings Long Nguyen, Andrzej Drozdzecki, Vera Goossens, Bert De Rybel, Tom Beeckman, and Dominique Audenaert 2. Novel Micro-Phenotyping Approach to Chemical Genetic Screening for Increased Plant Tolerance to Abiotic Stress Susan Fozard and Brian G. Forde 3. Method for Ultra-Rapid High-Content Screening for Biologically Active Chemicals Using Plant Pollen Elena Marusich, Roman Chuprov-Netochin, Yaroslav Neskorodov, Yana Mishutkina, Polina Volynchuk, Sergey Leonov, Yan Ivanenkov, Alisher Touraev, and Klaus Palme 4. A High-Throughput Chemical Screening Method for Inhibitors and Potentiators of Hypersensitive Cell Death using Suspension Cell Culture of Arabidopsis thaliana Yoshiteru Noutoshi and Ken Shirasu 5. High-Throughput Screening of Chemical Compound Libraries for Modulators of Salicylic Acid Signaling by In Situ Monitoring of Glucuronidase-Based Reporter Gene Expression Vivek Halder and Erich Kombrink 6. Identification of Chemical Inducers of the Phosphate-Starvation Signaling Pathway in A. thaliana using Chemical Genetics Clemence Bonnot, Laurent Nussaume, and Thierry Desnos 7. Phenotype-Based Screening of Small Molecules to Modify Plant Cell Walls using BY-2 Cells Emiko Okubo-Kurihara and Minami Matsui 8. Small Molecule Screening to Increase Agrobacterium-Mediated Transformation Efficiency in Legumes Mitsuhiro Kimura and Sachiko Isobe 9. High-Throughput In Vitro Screening for Inhibitors of Cereal -glucosidase Michael D. Rugen, Martin Rejzek, Henrik Naested, Birte Svensson, and Robert A. Field 10. Chemical Screening for Strigolactone Receptor Antagonists using Arabidopsis thaliana Duncan Holbrook-Smith and Peter McCourt 11. Chemical Control of ABA Receptors to Enable Plant Protection against Water Stress Masanori Okamoto and Sean R. Cutler 12. Modes of Action Study of Seed Germination Inhibitor Germostatin by Forward Genetics Screening Yajin Ye and Yang Zhao 13. Enantiodifferential Approach for the Target Protein Detection of the Jasmonate Glucoside that Controls the Leaf Closure of Samanea saman Yousuke Takaoka and Minoru Ueda 14. Using the QCM Biosensor-Based T7 Phage Display Combined with Bioinformatics Analysis for Target Identification of Bioactive Small Molecule Yoichi Takakusagi, Kaori Takakusagi, Fumio Sugawara, and Kengo Sakaguchi 15. Genomic Identification and Analysis of Specialized Metabolite Biosynthetic Gene Clusters in Plants using PlantiSMASH Satria A. Kautsar, Hernando G. Suarez Duran, and Marnix H. Medema 16. Chemical Genomics Translatability from Unicellular to Multicellular Models Cecilia Rodriguez-Furlan, Carlos Rubilar-Hernandez, and Lorena Norambuena 17. Chemical Screening for Flagella-Associated Phenotypes in Chlamydomonas reinhardtii Brittany Jack and Prachee Avasthi 18. FnCpf1-Mediated Targeted Mutagenesis in Plants Akira Endo and Seiichi Toki

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詳細情報

• NII書誌ID(NCID): BB29232483
• ISBN:
  • 9781493978731
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York
• ページ数/冊数: xi, 242 p.
• 大きさ: 27 cm
• 親書誌ID:
  • BA10794673
  • BA88873632