Extracellular RNA : methods and protocols

著者

    • Patel, Tushar

書誌事項

Extracellular RNA : methods and protocols

edited by Tushar Patel

（Methods in molecular biology / John M. Walker, series editor, 1740）（Springer protocols）

Humana Press, c2018

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内容説明・目次

内容説明

This volume provides a collection of methods and protocols detailing extracellular RNA and hopes to be a useful resource for those interested in this exciting and emerging field. Chapters are divided into four sections covering an overview of the field, approaches for the isolation of exRNA, protocols for detection and quantitation of exRNA, and approaches that can be useful for studies to evaluate potential therapeutic applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Extracellular RNA: Methods and Protocols aims to ensure successful results in the further study of this vital field.

目次

1. Extracellular RNAs - A New Awareness of Old Perspectives Noah Sadik, Lilian Cruz, Alessandra Gurtner, Rodosthenis S. Rodosthenous , Sophie A. Dusoswa, Olivia Ziegler, Thomas Sebastiaan Van Solinge, Zhiyun Wei, Ane Miren Salvador Garicano, Bence Gyorgy, Marike Broekman, and Leonora Balaj 2. Overview of Protocols for Studying Extracellular RNA and Extracellular Vesicles Julia Small, Roger Alexander, and Leonora Balaj 3. Extracellular RNA Isolation from Cell Culture Supernatant Sawen Bakr., Bridget Simonson, Kirsty M. Danielson, and Saumya Das 4. Use of a Hollow Fiber Bioreactor to Collect Extracellular Vesicles from Cells in Culture Irene K. Yan, Neha Shukla, David A. Borrelli, and Tushar Patel 5. Isolation of Extracellular RNA from Serum/Plasma Justyna Filant, Parham Nejad, Anu Paul, Bridget Simonson, Srimeenakshi Srinivasan, Xuan Zhang, Leonora Balaj, Saumya Das, Roopali Gandhi, Louise C. Laurent, and Anil K. Sood 6. Isolation of Extracellular RNA from Bile Irene K. Yan, Valentin X. Berdah, and Tushar Patel 7. Cushioned-Density Gradient Ultracentrifugation (C-DGUC): a Refined and High Performance Method for the Isolation, Characterization & Use of Exosomes Kang Li, David K. Wong, King Yeung Hong, and Robert L. Raffai 8. Magnetic Particle-Based Immunoprecipitation of Nanoscale Extracellular Vesicles from Biofluids Pete Heinzelman 9. Enrichment of Extracellular Vesicle Subpopulations via Affinity Chromatography Michelle E. Hung, Stephen B. Lenzini, Devin M. Stranford, and Joshua N. Leonard 10. Detection and Analysis of Non-vesicular Extracellular RNA Juan Pablo Tosar and Alfonso Cayota 11. Isolation of Plasma Lipoproteins as a Source of Extracellular RNA Kang Li, David K. Wong, Fu Sang Luk, Roy Y. Kim, and Robert L. Raffai 12. Droplet Digital PCR for Quantitation of Extracellular RNA Irene K Yan, Rishabh Lohray, and Tushar Patel 13. Preparation of Small RNA NGS Libraries from Biofluids Alton Etheridge, Kai Wang, David Baxter, and David Galas 14. Multiplexed Detection and Quantitation of Extracellular Vesicle RNA Expression using NanoString Neha Shukla, Irene K. Yan, and Tushar Patel 15. Milk-derived Extracellular Vesicles for Therapeutic Delivery of Small Interfering RNAs Akiko Matsuda and Tushar Patel 16. Loading of Extracellular Vesicles with Hydrophobically Modified siRNAs Marie-Cecile Didiot, Reka A. Haraszti, Neil Aronin, and Anastasia Khvorova

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