Plant and food carotenoids : methods and protocols
著者
書誌事項
Plant and food carotenoids : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 2083）（Springer protocols）
Humana Press, c2020
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This volume provides a comprehensive compilation of techniques and protocols used in plant and food carotenoid research. Chapters guide readers through seven major areas on core enzyme activities, analysis of carotenoid profiles, new imaging tools, synthesis and degradation dynamics, biotechnology, nutrition, and health. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Plant and Food Carotenoids: Methods and Protocols aims to be helpful to researchers of other disciplines that are impacted by carotenoids, including photosynthesis, biotechnology, food science, and nutrition.
目次
- Part I: Introduction 1. Pathways for Carotenoid Biosynthesis, Degradation, and Storage Tianhu Sun, Yaakov Tadmor, and Li Li Part II: Activities 2. A Simple in vitro Assay to Measure the Activity of Geranylgeranyl Diphosphate Synthase and Other Short-chain Prenyltransferases M. Victoria Barja and Manuel Rodriguez-Concepcion 3. Expression, Purification, and Enzyme Activity Assay of Phytoene Synthase in vitro Maurizio Camagna and Ralf Welsch 4. Improved Expression and Purification of the Carotenoid Biosynthetic Enzyme Z-ISO Eleanore T. Wurtzel and Jesus Beltran 5. Determination of In Vitro and In Vivo Activities of Plant Carotenoid Cleavage Oxygenases Lourdes Gomez-Gomez, Gianfranco Diretto, Oussama Ahrazem, Salim Al-Babili 6. Expression and Characterization of Mammalian Carotenoid Cleavage Dioxygenases Linda Thomas, Srinivasagan Ramkumar, and Johannes von Lintig 7. Transportomics for the Characterization of Plant Apocarotenoid Transmembrane Transporters Olivia Costantina Demurtas, Rita de Brito Francisco, Enrico Martinoia, and Giovanni Giuliano Part III: Analytics 8. Applications of Visible Spectroscopy and Colour Measurements in the Assessments of Carotenoid Levels in Foods Paula Mapelli-Brahm, Francisco J. Rodriguez-Pulido, Carla M. Stinco
- Francisco J. Heredia, and Antonio J. Melendez-Martinez 9. A Routine Method for the Extraction and HPLC-DAD Profiling of Major Plant and Food Carotenoids Damaso Hornero-Mendez 10. Acquisition of Mass Spectrometry Data of Carotenoids: A Focus on Big Data Management Antonio Perez-Galvez, Isabel Viera, and Maria Roca 11. cis/trans Carotenoid Extraction, Purification, Detection, Quantification, and Profiling in Plant Tissues Yagiz Alagoz, Namraj Dhami, Chris Mitchell, and Christopher I Cazzonelli 12. Determination of Plant Volatile Apocarotenoids Jose L. Rambla and Antonio Granell 13. A Method for Extraction and LC-MS Based Identification of Carotenoid-Derived Dialdehydes in Plants Jianing Mi, Kun-Peng Jia, Aparna Balakrishna, and Salim Al-Babili 14. Fractionation of Tomato Fruit Chromoplasts Karel De Pourcq and Albert Boronat 15. Quantification of Strigolactones Carlos Rial, Rosa M. Varela, Jose M. G. Molinillo, Alexandra G. Duran and Francisco A. Macias 16. Carotenoid and Apocarotenoid Analysis by SFE-SFC-QqQ/MS Daniele Giuffrida, Mariosimone Zoccali, and Luigi Mondello Part IV: Imaging 17. Elucidating Carotenoid Biosynthetic Enzyme Localization and Interactions Using Fluorescent Microscopy Maria Shumskaya, Rena F. Quinlan, and Eleanore T. Wurtzel 18. Visualization of Carotenoid-storage Structures in Fruits by Transmission Electron Microscopy Joanna Lado, Jaime Zacarias, Maria Jesus Rodrigo, and Lorenzo Zacarias 19. Light Microscopy and Raman Imaging of Carotenoids in Plant Cells in situ and in Released Carotene Crystals Tomasz Oleszkiewicz, Marta Z. Pacia, Ewa Grzebelus, and Rafal Baranski Part V: Dynamics 20. Carotenoid Isotopologue Profiling in 13C-labeled Leaf Extracts by LC-MS and LC-FTICR-MS Bjoern Thiele and Shizue Matsubara 21. Quantification of Carotenoid Pathway Flux in Green and Non-green Systems Julian Koschmieder and Ralf Welsch 22. Histochemical and Molecular Quantification of Arbuscular Mycorrhiza Symbiosis Juan M. Garcia, Maria J. Pozo, and Juan A. Lopez-Raez Part VI: Biotechnology 23. Use of Potyvirus Vectors to Produce Carotenoids in Plants Jose-Antonio Daros 24. Agrobacterium tumefaciens Mediated Stable Transformation of Daucus carota Christian Gonzalez-Calquin and Claudia Stange 25. CRISPR-Cas-Mediated Gene Knockout in Tomato Gwen Swinnen, Thomas Jacobs, Laurens Pauwels, and Alain Goossens 26. Modulation of Activity of a Carotenoid Pathway Through the use of the TET-on Regulatory System: Application in the Fungus Fusarium fujikuroi Julia Marente, Pedro Ortega, Javier Pardo-Medina, Javier Avalos, and M. Carmen Limon Part VII: Nutrition and Health 27. Intervention Studies in Humans Begona Olmedilla-Alonso 28. In vitro Digestion for Control and Monitoring of Food Effects In Relation To Micellarization Index of Carotenoids Antonio Perez-Galvez and Javier Fontecha 29. Evaluation of Carotenoids Protection against Oxidative Stress in the Animal Model Caenorhabditis elegans Patricia Martorell, Silvia Llopis, Jose Vicente Gil, Salvador Genoves, Daniel Ramon, Lorenzo Zacarias, and Maria Jesus Rodrigo 30. Mouse Models to Study Antiobesogenic Effects of Carotenoids Joan Ribot, Bojan Stojnic, Andreu Palou, and M. Luisa Bonet
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