Brain development : methods and protocols

著者

• Sprecher, Simon G.

書誌事項

Brain development : methods and protocols

edited by Simon G. Sprecher

（Methods in molecular biology / John M. Walker, series editor, 2047）（Springer protocols）

Humana Press, c2020

2nd ed

注記

Includes bibliographical references and index

内容説明・目次

内容説明

This book provides a thorough introduction to widely used techniques for the study of the intersection between developmental biology and neuroscience, an exceptional area to address and investigate impacting biological questions. The fully updated volume examines cutting-edge techniques on a representative range of animals, including widely used genetic model systems, such as the fruit fly, zebra fish, chicken, and mouse, as well as non-canonical experimental systems opened up through the advent of genome editing. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Brain Development: Methods and Protocols, Second Edition is an ideal guide for researchers interested in utilizing recent technical advances in molecular genetics for the study of the brain.

目次

Part I: Invertebrate Models 1. Combining BrdU-Labeling to Detection of Neuronal Markers to Monitor Adult Neurogenesis in Hydra Wanda Buzgariu, Marie-Laure Curchod, Chrystelle Perruchoud, and Brigitte Galliot 2. Reverse Genetic Approaches to Investigate the Neurobiology of the Cnidarian Sea Anemone Nematostella vectensis Jamie A. Havrilak and Michael J. Layden 3. Generating Transgenic Reporter Lines for Studying Nervous System Development in the Cnidarian Nematostella vectensis Fabian Rentzsch, Eduard Renfer, and Ulrich Technau 4. Immunostaining and In Situ Hybridization of the Developing Acoel Nervous System Elena Perea-Atienza, Brenda Gavilan, Simon G. Sprecher, and Pedro Martinez 5. Immunostaining of the Embryonic and Larval Drosophila Brain Frank Hirth and Danielle C. Diaper 6. Non-Fluorescent RNA In Situ Hybridization Combined with Antibody Staining to Visualize Multiple Gene Expression Patterns in the Embryonic Brain of Drosophila David Jussen and Rolf Urbach 7. Analysis of Complete Neuroblast Cell Lineages in the Drosophila Embryonic Brain via DiI Labeling Karoline F. Kraft and Rolf Urbach 8. Flybow to Dissect Circuit Assembly in the Drosophila Brain: An Update Emma L. Powell and Iris Salecker 9. Live Cell Imaging of Neural Stem Cells in the Drosophila Larval Brain Karolina Miszczak and Boris Egger 10. CRISPR/Cas9 Genome Editing to Study Nervous System Development in Drosophila Cornelia Fritsch and Simon G. Sprecher 11. The Red Flour Beetle as Model for Comparative Neural Development: Genome Editing to Mark Neural Cells in Tribolium Brain Development Max S. Farnworth, Kolja N. Eckermann, Hassan M.M. Ahmed, Dominik S. Muhlen, Bicheng He, and Gregor Bucher 12. A Protocol for Double Fluorescent In Situ Hybridization and Immunohistochemistry for the Study of Embryonic Brain Development in Tribolium castaneum Marita Buscher, Georg Oberhofer, Natalia Carolina Garcia-Perez, and Gregor Bucher 13. Immunohistochemistry and Fluorescent Whole Mount RNA In Situ Hybridization in Larval and Adult Brains of Tribolium Vera S. Hunnekuhl, Max S. Farnworth, Bicheng He, and Gregor Bucher 14. X-Ray Microscopy of the Larval Crustacean Brain Jakob Krieger and Franziska Spitzner 15. Immunolocalization of Neurotransmitters and Neuromodulators in the Developing Crayfish Brain Steffen Harzsch and Caroline Viertel 16. Immunostainings in Nervous System Development of the Nematode C. elegans Janet S. Duerr 17. Methods in Brain Development of Molluscs Andreas Wanninger and Tim Wollesen 18. A Simple Method to Identify Ascidian Brain Lineage Cells at Neural Plate Stages Following In Situ Hybridization Clare Hudson 19. Spawning Induction and Embryo Micromanipulation Protocols in the Amphioxus Branchiostoma lanceolatum Yann Le Petillon, Stephanie Bertrand, and Hector Escriva Part II: Vertebrate Models 20. In Situ Hybridization and Immunostaining of Xenopus Brain Kai-li Liu, Xiu-mei Wang, Zi-long Li, Ying Liu, and Rong-qiao He 21. Morpholino Studies in Xenopus Brain Development Jennifer E. Bestman and Hollis T. Cline 22. Sensitive Multiplexed Fluorescent In Situ Hybridization Using Enhanced Tyramide Signal Amplification and Its Combination with Immunofluorescent Protein Visualization in Zebrafish Gilbert Lauter, Iris Soell, and Giselbert Hauptmann 23. Live Morphometric Classification of Sensory Neurons in Larval Zebrafish Gema Valera and Hernan Lopez-Schier 24. Immunohistochemistry and In Situ Hybridization in the Developing Chicken Brain Richard P. Tucker, Tatsuto Ishimaru, and Qizhi Gong 25. Gene Silencing in Chicken Brain Development Georgia Tsapara, Irwin Andermatt, and Esther T. Stoeckli 26. Transplantation of Neural Tissue: Quail-Chick Chimeras Andrea Streit and Claudio D. Stern 27. Immunohistochemistry and RNA In Situ Hybridization in Mouse Brain Development Jinling Liu and Aimin Liu 28. The Cre/Lox System to Assess the Development of the Mouse Brain Claudius F. Kratochwil and Filippo M. Rijli 29. In Utero Electroporation to Study Mouse Brain Development Emilie Pacary and Francois Guillemot

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詳細情報

• NII書誌ID(NCID): BB2948484X
• ISBN:
  • 9781493997312
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York
• ページ数/冊数: xiv, 527 p.
• 大きさ: 27 cm
• 分類:
  • LCC : QP376
  • DC23 : 573.8/6
• 件名:
  • LCSH : Brain -- Growth -- Laboratory manuals
• 親書誌ID:
  • BA10794673
  • BA88873632