Cyclic nucleotide signaling in plants : methods and protocols
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Bibliographic Information
Cyclic nucleotide signaling in plants : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1016）（Springer protocols）
Humana Press, 2013
- : softcover
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"Softcover reprint of the hardcover 1st edition 2013"--T.p. verso
Includes bibliographical references and index
Description and Table of Contents
Description
Over the last two decades there has been a growing interest in cyclic nucleotide research in plants with an emphasis on the elucidation of the roles of cGMP and cAMP. In Cyclic Nucleotide Signaling in Plants: Methods and Protocols, expert researchers in the field detail many approaches to better understand the biological role of this important signaling system. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Cyclic Nucleotide Signaling in Plants: Methods and Protocols seeks to aid scientist in further understanding signal transduction and the molecular mechanisms underlying cellular signaling.
Table of Contents
1: Molecular Methods for the Study of Signal Transduction in Plants
Helen R. Irving and Chris Gehring
2: Recombinant Expression and Functional Testing of Candidate Adenylate Cyclase Domains
Oziniel Ruzvidzo, Bridget T. Dikobe, David T. Kawadza, Grace H. Mabadahanye, Patience Chatukuta, Lusisizwe Kwezi
3: Quantification of Cyclic Dinucleotides by Reversed-Phase LC-MS/MS
Heike Burhenne and Volkhard Kaever
4: Determination of ADP-ribosyl Cyclase Activity, Cyclic ADP-ribose and Nicotinamide Adenine Dinucleotide Phosphate in Tissue Extracts
Richard M. Graeff and Hon Cheung Lee
5: In vivo Imaging of cGMP in Plants
Jean-Charles Isner and Frans J.M. Maathuis
6: Characterization of Heterologously Expressed Transporter Genes by Patch- and Voltage-Clamp Methods: Application to Cyclic Nucleotide-Dependent Responses
Fouad Lemtiri-Chlieh and Rashid Ali
7: Non-invasive Microelectrode Ion Flux Estimation Technique (MIFE) for the Study of the Regulation of Root Membrane Transport by Cyclic Nucleotides
Natalia Maria Ordonez, Lana Shabala, Chris Gehring, and Sergey Shabala
8: Calcium Imaging of the Cyclic Nucleotide Response
Martin R. McAinsh, Stephen K. Roberts and Lyudmila V. Dubovskaya
9: Identification and Quantification of Signal Molecule-dependent Protein Phosphorylation
Arnoud Groen, Ludivine Thomas, Kathryn Lilley and Claudius Marondedze
10: Comparative Gel-based (Phospho)Proteomics in Response to Signaling Molecules
Claudius Marondedze, Kathryn Lilley and Ludivine Thomas
11: An Affinity Pull-down Approach to Identify the Plant Cyclic Nucleotide Interactome
Lara Donaldson and Stuart Meier
12: Structural and Functional Characterization of Receptor Kinases with Nucleotide Cyclase Activity
Victor Muleya, Janet I. Wheeler and Helen R. Irving
13: Computational Identification of Candidate Nucleotide Cyclases in Higher Plants
Aloysius Wong and Chris Gehring
14: Identification of Cyclic Nucleotide Gated Channels Using Regular Expressions
Alice K. Zelman, Adam Dawe and Gerald A. Berkowitz
15: Inferring biological functions of guanylyl cyclases with computational methods
May Alqurashi and Stuart Meier
16: Identification and Characterization of Cyclic Nucleotide Phosphodiesterases
Erin B. Purcell and Rita Tamayo
17: Detection of reactive oxygen species down-stream of cyclic nucleotide signals in plants
Robin K. Walker and Gerald A. Berkowitz
18: Measurement of Nitric Oxide in Plant Tissue using Difluorofluorescein and Oxyhaemoglobin
Ndiko Ludidi
19: Infra-red Gas Analysis (IRGA) Technique for the Study of the Regulation of Photosynthetic Responses
Alex Valentine, Oziniel Ruzvidzo, Aleysia Kleinert, Yun Kang and Vagner Bennedito
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