PCR : methods and protocols
著者
書誌事項
PCR : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1620）（Springer protocols）
Humana Press, c2017
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This volume details PCR technique with focus on its application specificities to the biotechnology and bioengineering field. Chapters are broken into five sections covering sgeneral PCR protocols, different applied examples to molecular and synthetic biotechnology, food science and technology, environmental microbiology and molecular ecology, and healthcare. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, PCR: Methods and Protocols hopes to be a valuable and useful resource for wet lab researchers, particularly within the biotechnology and bioengineering field.
目次
1. In silico PCR Tools for a Fast Primer, Probe and Advanced Searching Ruslan Kalendar, Alexandr Muterko, Malika Shamekova, and Kabyl Zhambakin
2. Introduction on using the FastPCR Software and the Related Java Web Tools for PCR and Oligonucleotide Assembly and Analysis
Ruslan Kalendar and Timofey V. Tselykh
3. Long Fragment Polymerase Chain Reaction
Eng Wee Chua, Simran Maggo, and Martin A. Kennedy
4. Strategies to Improve Efficiency and Specificity of Degenerate Primers in PCR
Maria Jorge Campos and Alberto Quesada
5. Inverse PCR for Point Mutation Introduction
Diogo Silva, Gustavo Santos, Mario Barroca, and Tony Collins
6. Synthesis of Fusion Genes for Cloning by Megaprimer-based PCR
Tatiana Q. Aguiar, Carla Oliveira, and Lucilia Domingues
7. A Novel Platform for High-throughput Gene Synthesis to Maximize Recombinant Expression in Escherichia coli
Ana Filipa Sequeira, Joana L.A. Bras, Vania O. Fernandes, Catarina I.P.D. Guerreiro, Renaud Vincentelli, and Carlos M.G.A. Fontes
<8. Colony PCR
Flavio Azevedo, Humberto Pereira, and Bjoern Johansson
9. CRENAME, a Molecular Microbiology Method Enabling Multiparametric Assessment of Potable/Drinking Water
Luc Bissonnette, Andree F. Maheux, and Michel G. Bergeron
10. Multiplex Detection Of Food-borne Pathogen
German Villamizar-Rodriguez and Felipe Lombo
11. Fast Real-time PCR for the Detection of Crustacean Allergens in Foods
Francisco J. Santaclara and Montserrat Espineira
12. Fast Real-time PCR Method for the Detection of Soy in Foods
Montserrat Espineira and Francisco J. Santaclara
13. RAPD/SCAR Approaches for Identification of Adulterant's Breed's Milk in Dairy Products
Joana Cunha and Lucilia Domingues
14. Genetic Diversity Analysis of Medicinally Important Horticultural Crop Aegle marmelos by ISSR Markers
Farina Mujeeb, Preeti Bajpai, Neelam Pathak, and Smita Rastogu Verma
15. PCR in the Analysis of Clinical Samples: Prenatal and Postnatal Diagnosis of Inborn Errors of Metabolism
Laura Vilarinho and Celia Nogueira
16. Harnessing the Power of PCR-dependent Molecular Fingerprinting Methods for Understanding Structure and Function in Microbial Communities
S.S. Phadke, A.F. Salvador, J.I. Alves, O. Bretschger, M.M. Alves, and M.A. Pereira
17. PCR in Metagenomics
Sarita G Bhat and Tina K. J.
18. Arbitrarily Primed PCR for Comparison of Metagenomes and Extracting useful loci from them
Leigh Burgoyne, Lin Y Koh, and David Catcheside
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