Tumor immunology and immunotherapy : cellular methods
著者
書誌事項
Tumor immunology and immunotherapy : cellular methods
（Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan, v. 631-632）
Academic Press, 2020
1st ed
- pt. A
- pt. B
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注記
Includes bibliographical references
内容説明・目次
- 巻冊次
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pt. A ISBN 9780128186732
内容説明
Tumor Immunology and Immunotherapy - Cellular Methods Part A, Volume 631, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. New chapters include Detection of intracellular cytokine production by T cells with flow cytometry, High-throughput identification of human antigen-specific CD8+ and CD4+ T cells using soluble pMHC multimers, In vitro assays for effector T cell functions and activity of immunostimulatory antibodies, Ex vivo energetic profiling of tumor cells and T cells from mouse models and human samples, A cytofluorimetric assay to evaluate T cell polyfunctionality, and much more.
目次
1. Cytokine profiling of tumor-infiltrating T lymphocytes by flow cytometry Jeremy B. Foote, Sujith Sarvesh and Leisha A. Emens 2. High-throughput identification of human antigen-specific CD8+ and CD4+ T cells using soluble pMHC multimers Morgane Magnin, Philippe Guillaume, George Coukos, Alexandre Harari and Julien Schmidt 3. In vitro assays for effector T cell functions and activity of immunomodulatory antibodies Roberta Zappasodi, Sadna Budhu, Mohsen Abu-Akeel and Taha Merghoub 4. A cytofluorimetric assay to evaluate T cell polyfunctionality Belinda Palermo, Mariangela Panetta, Giulia Campo and Paola Nistico 5. Assessment of memory formation by metabolically engineered antigen-specific CD8 T cells Mathias Wenes, Pedro Romero and Lianjun Zhang 6. Functional characterization of tumor antigen-specific T-cells isolated from the tumor microenvironment of sleeping beauty induced murine glioma models Mahmoud S. Alghamri, Felipe J. Nunez, Neha Kamran, Stephen Carney, David Altshuler, Pedro R. Lowenstein and Maria G. Castro 7. Methods to edit T cells for cancer immunotherapy Francesca Lucibello, Silvia Menegatti and Laurie Menger 8. Generating stem-like memory T cells with antioxidants for adoptive cell transfer immunotherapy of cancer Karolina Pilipow, Eloise Scamardella and Enrico Lugli 9. Reverse immunology: From peptide sequence to tumor-killing human T-cell clones Christophe Vanhaver, Monica Gordon-Alonso, Alexandre Bayard, Maria Teresa Catanese, Didier Colau, Pierre van der Bruggen and Annika M. Bruger 10. Generation of TCR-engineered reference cell samples to control T-cell assay performance Nicole Bidmon, Cecile Gouttefangeas and Sjoerd H. van der Burg 11. In vitro expansion of V?9Vd2 T cells for immunotherapy Christian Peters, Leonce Kouakanou, Hans-Heinrich Oberg, Daniela Wesch and Dieter Kabelitz 12. CFSE dilution to study human T and NK cell proliferation in vitro Inigo Terren, Ane Orrantia, Joana Vitalle, Olatz Zenarruzabeitia and Francisco Borrego 13. Rapid isolation and enrichment of mouse NK cells for experimental purposes Maite Alvarez, Maria C. Ochoa, Luna Minute, Ignacio Melero and Pedro Berraondo 14. Assessment of NK cell-mediated cytotoxicity by flow cytometry after rapid, high-yield isolation from peripheral blood Peter Holicek, Iva Truxova, Lenka Kasikova, Sarka Vosahlikova, Cyril Salek, Jana Rakova, Monika Holubova, Daniel Lysak, Isabelle Cremer, Radek Spisek and Jitka Fucikova 15. Two-dimensional dynamic evaluation of natural killer cell-mediated lysis of adherent target cells Alexandra Frazao, Louise Rethacker and Anne Caignard 16. Rapid isolation of mouse ILCs from murine intestinal tissues Kyle Burrows, Pailin Chiaranunt, Louis Ngai and Arthur Mortha 17. Detecting and analyzing murine innate lymphoid cells Alejandra Gomez-Cadena, Pedro Romero, Sara Trabanelli and Camilla Jandus 18. A cytofluorimetric assay to evaluate intracellular cytokine production by NK cells Christian Sordo-Bahamonde, Seila Lorenzo-Herrero, Segundo Gonzalez and Alejandro Lopez-Soto 19. Applications of microfluidic devices in advancing NK-cell migration studies Xiaoou Ren, Abdulaziz Alamri, Jolly Hipolito, Francis Lin and Sam K.P. Kung 20. Complementary approaches to study NKT cells in cancer Jay A. Berzofsky, Purevdorj B. Olkhanud and Masaki Terabe 21. Assessment of IFN-? and granzyme-B production by in "sitro" technology Claudia Galassi, Gwenola Manic, Martina Musella, Antonella Sistigu and Ilio Vitale 22. Assessment of IFN? responsiveness in patient-derived xenografts Jordan J. Cardenas, Camila Robles-Oteiza and Katerina Politi 23. Real-time cell analysis (RTCA) to measure killer cell activity against adherent tumor cells in vitro Hans-Heinrich Oberg, Christian Peters, Dieter Kabelitz and Daniela Wesch 24. Analysis of cancer cell-intrinsic immune regulation in response to CD8+ T cell attack Natalie J. Neubert, Laure Tille, Christophe Martignier, Silvia A. Fuertes Marraco and Daniel E. Speiser 25. A flow cytometry-based method to screen for modulators of tumor-specific T cell cytotoxicity Javier Santos, Jesus Ogando, Rosa Ana Lacalle and Santos Manes 26. Evaluation of NK cell cytotoxic activity against malignant cells by the calcein assay Seila Lorenzo-Herrero, Christian Sordo-Bahamonde, Segundo Gonzalez and Alejandro Lopez-Soto 27. 51Cr-release to monitor NK cell cytotoxicity Leslie Elsner and Ralf Dressel
- 巻冊次
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pt. B ISBN 9780128186756
内容説明
Tumor Immunology and Immunotherapy - Cellular Methods Part B, Volume 632, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Topics covered include Quantitation of calreticulin exposure associated with immunogenic cell death, Side-by-side comparisons of flow cytometry and immunohistochemistry for detection of calreticulin exposure in the course of immunogenic cell death, Quantitative determination of phagocytosis by bone marrow-derived dendritic cells via imaging flow cytometry, Cytofluorometric assessment of dendritic cell-mediated uptake of cancer cell apoptotic bodies, Methods to assess DC-dependent priming of T cell responses by dying cells, and more.
目次
1. Quantitation of calreticulin exposure associated with immunogenic cell death Peng Liu, Liwei Zhao, Oliver Kepp and Guido Kroemer 2. Side-by-side comparison of flow cytometry and immunohistochemistry for detection of calreticulin exposure in the course of immunogenic cell death Lenka Kasikova, Iva Truxova, Isabelle Cremer, Catherine Sautes-Fridman, Oliver Kepp, Guido Kroemer, Radek Spisek and Jitka Fucikova 3. Quantitative determination of phagocytosis by bone marrow-derived dendritic cells via imaging flow cytometry Giulia Cerrato, Peng Liu, Isabelle Martins, Oliver Kepp and Guido Kroemer 4. Cytofluorometric assessment of dendritic cell-mediated uptake of cancer cell apoptotic bodies Martina Musella, Gwenola Manic, Claudia Galassi, Ilio Vitale and Antonella Sistigu 5. Methods to assess DC-dependent priming of T cell responses by dying cells Anne-Marie Chalupova, Sarka Vosahlikova, Daniela Rozkova, Klara Sochorova, Lenka Palova-Jelinkova, Jirina Bartunkova, Radek Spisek and Jitka Fucikova 6. Isolation of mononuclear phagocytes from the mouse gut Pailin Chiaranunt, Kyle Burrows, Louis Ngai and Arthur Mortha 7. Generation of murine bone marrow-derived macrophages and using tumor coculture assays to mimic tumor microenvironment Esra Guc, Demi Brownlie, Carolina Rodriguez Tirado, Takanori Kitamura and Jeffrey W. Pollard 8. Methods for macrophage differentiation and in vitro generation of human tumor associated-like macrophages Esther Heideveld, Marta Horcas-Lopez, Martha Lopez-Yrigoyen, Lesley M. Forrester, Luca Cassetta and Jeffrey W. Pollard 9. In vitro assessment of cancer cell-induced polarization of macrophages Danique E.M. Duits, Max D. Wellenstein and Karin E. de Visser 10. Protocol to assess the suppression of T-cell proliferation by human MDSC Annika M. Bruger, Christophe Vanhaver, Kirsten Bruderek, Giada Amodio, Ece Tavukcuoglu, Gunes Esendagli, Silvia Gregori, Sven Brandau and Pierre van der Bruggen 11. Detection and functional evaluation of arginase-1 isolated from human PMNs and murine MDSC Stefania Cane and Vincenzo Bronte 12. Functional assay to assess T-cell inhibitory properties of myeloid derived suppressor cells (MDSCs) isolated from the tumor microenvironment of murine glioma models Mahmoud S. Alghamri, Neha Kamran, Padma Kadiyala, Pedro R. Lowenstein and Maria G. Castro 13. FACS isolation and analysis of human circulating and tumor neutrophils Agnieszka Swierczak and Jeffrey W. Pollard 14. Flow cytometry-based isolation of tumor-associated regulatory T cells and assessment of their suppressive potential Kevin Kos, Martijn van Baalen, Denize A. Meijer and Karin E. de Visser 15. Assessment of lipid load in tumor-infiltrating Tregs by flow cytometry Ilenia Pacella, Alfonso Grimaldi and Silvia Piconese 16. Preparation of single cells from tumors for single-cell RNA sequencing Yonina Bykov, Sarah H. Kim and Dmitriy Zamarin 17. Characterization of the tumor immune infiltrate by multiparametric flow cytometry and unbiased high-dimensional data analysis Romina E. Araya and Romina S. Goldszmid 18. Phenotyping of tumor infiltrating immune cells using mass-cytometry (CyTOF) Pauline Maby, Aurelien Corneau and Jerome Galon 19. Isolation and characterization of immune cells from the tumor microenvironment of genetically engineered pediatric high-grade glioma models using the sleeping beauty transposon system Maria Belen Garcia-Fabiani, Andrea Comba, Padma Kadiyala, Santiago Haase, Felipe Javier Nunez, David Altshuler, Pedro Ricardo Lowenstein and Maria Graciela Castro 20. Analysis of the immune status from peripheral whole blood with a single-tube multicolor flow cytometry assay Anna-Jasmina Donaubauer, Ina Becker, Paul F. Ruhle, Rainer Fietkau, Udo S. Gaipl and Benjamin Frey 21. Ex vivo assessment of in vivo DC-targeted antibodies in pre-clinical models Qingrong Huang and Niroshana Anandasabapathy 22. Flow cytometry-based assessment of direct-targeting anti-cancer antibody immune effector functions Michelle L. Miller and Olivera J. Finn 23. Enzyme-linked immunospot assay to monitor antigen-specific cellular immune responses in mouse tumor models Donatella Negri, Paola Sestili, Martina Borghi, Maria Ciccolella and Laura Bracci 24. High-throughput analysis of cell-cell crosstalk in ad hoc designed microfluidic chips for oncoimmunology applications Arianna Mencattini, Adele De Ninno, Jacopo Mancini, Luca Businaro, Eugenio Martinelli, Giovanna Schiavoni and Fabrizio Mattei 25. Generation of affinity ranged antigen-expressing tumor cell lines Amaia Martinez-Usatorre and Pedro Romero 26. Recombinant fusion proteins for targeting dendritic cell subsets in therapeutic cancer vaccine Stephanie Corgnac, Natalia K. Botelho, Alena Donda and Pedro Romero
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