Plant microRNAs : methods and protocols
著者
書誌事項
Plant microRNAs : methods and protocols
(Methods in molecular biology / John M. Walker, series editor, 1932)(Springer protocols)
Humana Press, c2019
- : hbk
大学図書館所蔵 全1件
  青森
  岩手
  宮城
  秋田
  山形
  福島
  茨城
  栃木
  群馬
  埼玉
  千葉
  東京
  神奈川
  新潟
  富山
  石川
  福井
  山梨
  長野
  岐阜
  静岡
  愛知
  三重
  滋賀
  京都
  大阪
  兵庫
  奈良
  和歌山
  鳥取
  島根
  岡山
  広島
  山口
  徳島
  香川
  愛媛
  高知
  福岡
  佐賀
  長崎
  熊本
  大分
  宮崎
  鹿児島
  沖縄
  韓国
  中国
  タイ
  イギリス
  ドイツ
  スイス
  フランス
  ベルギー
  オランダ
  スウェーデン
  ノルウェー
  アメリカ
注記
Includes bibliographical references and index
内容説明・目次
内容説明
This detailed volume provides a collection of protocols for the study of miRNA functions in plants. Beginning with coverage of miRNA function, biogenesis, activity, and evolution in plants, the book continues by guiding readers through methods on the identification and detection of plant miRNAs, bioinformatic analyses, and strategies for functional analyses of miRNAs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Plant MicroRNAs: Method and Protocols aims to ensure successful results in the further study of this vital area of plant science.
目次
1. An Introduction to Methods for Discovery and Functional Analysis of MicroRNAs in Plants
Alma Armenta-Medina and C. Stewart Gillmor
2. The Plant MicroRNA Pathway: The Production and Action Stages
Joseph L. Pegler, Christopher P. Grof, and Andrew L. Eamens
3. Plant miRNA Conservation and Evolution
Lydia Gramzow and Gunter Theissen
4. Bioinformatic Analysis of Small RNA Sequencing Libraries
Ricardo A. Chavez Montes, Fabiola Jaimes Miranda, and Stefan de Folter
5. An Overview of miRNA and miRNA Target Analysis Tools
Anna Lukasik and Piotr Zielenkiewicz
6. miRLocator: A Python Implementation and Web Server for Predicting miRNAs from Pre-miRNA Sequences
Ting Zhang, Lie Ju, Jingjing Zhai, Yujia Song, Jie Song, and Chuang Ma
7. Prediction of Plant miRNA Targets
Priyanka Pandey, Prashant K. Srivastava, and Shree P. Pandey
8. Isolation and Detection Methods of Plant miRNAs
Pedro Fernando Vera Hernandez, Stefan de Folter, and Flor de Fatima Rosas Cardenas
9. Northern Blot Analysis of MicroRNAs and Other Small RNAs in Plants
Carlos De la Rosa and Jose Luis Reyes
10. miRNA Detection by Stem-Loop RT-qPCR in Studying MicroRNA Biogenesis and MicroRNA Responsiveness to Abiotic Stresses
Aleksandra Smoczynska, Pawel Sega, Agata Stepien, Katarzyna Knop, Artur Jarmolowski, Andrzej Pacak, and Zofia Szweykowska-Kulinska
11. Detection of miRNAs by Tissue Printing and Dot Blot Hybridization
Marcelino Martinez Nunez, Stefan de Folter, and Flor de Fatima Rosas Cardenas
12. In Situ Localization of Small RNAs in Plants
Cristina F. Marco, Damianos S. Skopelitis, and Marja C.P. Timmermans
13. Expression Pattern of Plant miRNAs by Classical Transcriptional Fusion Constructs
Andrea Tovar-Aguilar, Karla A. Sanchez-Elizondo, Alejandra Rodriguez-Rodriguez, Marcos I. Gonzalez-Jaime, Genaro Patino-Lopez, Vadim Perez-Koldenkova, Jesus A. Badillo-Corona, and Noe Duran-Figueroa
14. A Simple Protocol for Imaging Floral Tissues of Arabidopsis with Confocal Microscopy
Andrea Gomez-Felipe and Stefan de Folter
15. Degradome Sequencing in Plants
Shih-Shun Lin, Yihua Chen, and Mei-Yeh Jade Lu
16. Functional Analysis of Root MicroRNAs by a Constitutive Overexpression Approach in a Composite Plant System
Damien Formey, Jose Angel Martin-Rodriguez, and Georgina Hernandez
17. The Use of MicroRNA Decoy Technologies to Inhibit miRNA Function in Arabidopsis
Gigi Wong and Anthony A. Millar
18. Mimicry Technology: A Versatile Tool for Small RNA Suppression
Luis Manuel Villar-Martin and Ignacio Rubio-Somoza
19. Design and High-Throughput Generation of Artificial Small RNA Constructs for Plants
Alberto Carbonell
20. Detection of MicroRNA Processing Intermediates through RNA Ligation Approaches
Belen Moro, Arantxa M.L Rojas, and Javier F. Palatnik
21. Analysis of Methylation Status of Plant MicroRNAs
Susu Chen and Guodong Ren
22. Detection of Slicer Activity by Immunopurified Plant ARGONAUTE1
Laura Arribas-Hernandez, Maria Louisa Vigh, and Peter Brodersen
23. The Isolation of Total and Membrane-Bound Polysomes from Arabidopsis and the Detection of Their Associated AGO1 and sRNAs
Shengben Li
24. Immunolocalization to Study ARGONAUTE Proteins in Developing Ovules of the Brassicaceae
Gloria Leon-Martinez, Edgar Demesa-Arevalo, and Jean-Philippe Vielle-Calzada
25. Detection of Histone Modifications Associated with miRNAs
Sara Hernandez-Castellano and Clelia De-la-Pena
「Nielsen BookData」 より