MicroRNA target identification : methods and protocols
著者
書誌事項
MicroRNA target identification : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, v. 1970）（Springer protocols）
Humana Press, c2019
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This detailed book provides a comprehensive state-of-the-art presentation of all aspects of miRNA target identification, from the prediction of miRNA binding sites on their target molecules to their experimental validation and downstream functional analysis. It also explores methodologies and tools for target prediction and related analysis, as well as tutorials on specific tasks, tools, and analysis pipelines. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, MicroRNA Target Identification: Methods and Protocols updates on basic and advanced concepts involving miRNA target identification and delivers an extremely useful guide to specific computational tasks and analysis in miRNA research.
目次
1. A Practical Guide to miRNA Target Prediction
Most Mauluda Akhtar, Luigina Micolucci, Md Soriful Islam, Fabiola Olivieri, and Antonio Domenico Procopio
2. miRandb: A Meta-Database of Online Resources of miRNA and miRNA Targets
Seyed Hamid Aghaee-Bakhtiari
3. Sfold Tools for MicroRNA Target Prediction
William Rennie, Shaveta Kanoria, Chaochun Liu, C. Steven Carmack, Jun Lu, and Ye Ding
4. The Role of Tertiary Structure in MicroRNA Target Recognition
Hin Hark Gan and Kristin C. Gunsalus
5. Protocols for miRNA Target Prediction in Plants
Gaurav Sablok, Kun Yang, and Xiaopen Wen
6. Improving miRNA-Target Prediction Using CLASH Data
Xiaoman Li and Haiyan Hu
7. Integration of Omics Data to Identify Cancer-Related MicroRNA
Luciano Cascione
8. Functional Analysis of Genetic Variants and Somatic Mutations Impacting MicroRNA-Target Recognition: Bioinformatics Resources
Jesse D. Ziebarth, Anindya Bhattacharya, and Yan Cui
9. Construction and Analysis of miRNA Regulatory Networks
Antonella Mensi, Vincenzo Bonnici, Simone Caligola, and Rosalba Giugno
10. Identification of Disease-miRNA Networks across Different Cancer Types Using SWitchMiner (SWIM)
Giulia Fiscon, Federica Conte, Lorenzo Farina, Marco Pellegrini, Francesco Russo, and Paola Paci
11. Computational Methods to Investigate the Impact of miRNAs on Pathways
Salvatore Alaimo, Giovanni Micale, Alessandro La Ferlita, Alfredo Ferro, and Alfredo Pulvirenti
12. Consensus Target Prediction with Enrichment Analysis for MicroRNAs Harboring Editing Sites and Other Variations
Giovanni Nigita, Rosario Distefano, Dario Veneziano, Giulia Romano, Mario Acunzo, and Carlo M. Croce
13. A Roadmap for the Computational Prediction and Experimental Validation of Competitive Endogenous RNAs
Florian A. Karreth
14. Investigating miRNA-lncRNA Interactions: Computational Tools and Resources
Dario Veneziano, Gioacchino P. Marceca, Sebastiano Di Bella, Giovanni Nigita, Rosario Distefano, and Carlo M. Croce
15. Combing the Hairball: Improving Visualization of miRNA-Target Interaction Networks
Francesco Russo, Jessica Xin Hu, Jose Alejandro Romero Herrera, and Soren Brunak
16. Inconsistencies and Limitations of Current MicroRNA Target Identification Methods
Sophie Mockly and Herve Seitz
17. Experimental Validation of MicroRNA Targets: Luciferase Reporter Assay
Luisa Tomasello, Landon Cluts, and Carlo M. Croce
18. Experimental Validation of MicroRNA Targets: Mutagenesis of the Binding Regions
Luisa Tomasello, Landon Cluts, and Carlo M. Croce
19. Experimental Validation of MicroRNA Targets: Analysis of MicroRNA Targets through Western Blotting
Luisa Tomasello, Landon Cluts, and Carlo M. Croce
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