Plant transcription factors : methods and protocols

著者

    • 山口, 暢俊 ヤマグチ, ノブトシ

書誌事項

Plant transcription factors : methods and protocols

edited by Nobutoshi Yamaguchi

（Methods in molecular biology / John M. Walker, series editor, 1830）（Springer protocols）

Humana Press, c2018

大学図書館所蔵 件 / 全1件

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

This detailed book provides general protocols and technologies that plant biologists worldwide often utilize for the purpose of accelerating research progress in the field of plant transcription factors. Beginning with a brief introduction, the volume continues by exploring methods in the preparation of plant materials, detection of expression levels, interaction tests, and chromatin analyses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Transcription Factors: Methods and Protocols aims to answer a wide range of questions related to transcription factors commonly raised by plant biologists.

目次

Part I: Review and Overview Chapters 1. Master Regulatory Transcription Factors in Plant Development: A Blooming Perspective Kerstin Kaufmann and Chiara A. Airoldi 2. Application of CRISPR/Cas to Understand Cis- and Trans-Regulatory Elements in Plants Felix Wolter and Holger Puchta 3. The Long-Term 'In Natura' Study Sites of Arabidopsis halleri for Plant Transcription and Epigenetic Modification Analyses in Natural Environments Hiroshi Kudoh, Mie N. Honjo, Haruki Nishio, and Jiro Sugisaka Part II: Preparation of Plant Materials 4. Generation of Inducible Transgenic Lines of Arabidopsis Transcription Factors Regulated by MicroRNAs Pooja Aggarwal, Krishna Reddy Challa, Monalisha Rath, Preethi Sunkara, and Utpal Nath 5. A Specific Knockdown of Transcription Factor Activities in Arabidopsis Beibei Zheng, Bennett Thomson, and Frank Wellmer 6. Using CRISPR/Cas9 System to Introduce Targeted Mutation in Arabidopsis Ze Hong Lee, Nobutoshi Yamaguchi, and Toshiro Ito 7. CRISPR/Cas9-Based Genome Editing of Transcription Factor Genes in Marchantia polymorpha Shigeo S. Sugano and Ryuichi Nishihama 8. Cell-Type-Specific Promoter Identification Using Enhancer Trap Lines Tatyana Radoeva, Shunsuke Saiga, and Dolf Weijers 9. Isolation of Arabidopsis Palisade and Spongy Mesophyll Cells Kyohei Uemoto, Takashi Araki, and Motomu Endo 10. Ectopic Vascular Induction in Arabidopsis Cotyledons for Sequential Analysis of Phloem Differentiation Alif Meem Nurani, Yuki Kondo, and Hiroo Fukuda Part III: Detection of Expression Levels 11. High Impact Gene Discovery: Simple Strand Specific mRNA Library Construction and Differential Regulatory Analysis Based on Gene Co-Expression Network Yasunori Ichihashi, Atsushi Fukushima, Arisa Shibata, and Ken Shirasu 12. Laser Capture Micro-Dissection Coupled to RNA Sequencing: A Powerful Approach Applied to the Model Legume Medicago truncatula in Interaction with Sinorhizobium meliloti Brice Roux, Nathalie Rodde, Sandra Moreau, Marie-Francoise Jardinaud, and Pascal Gamas 13. nanoCAGE-XL: An Approach to High-Confidence Transcription Start Site Sequencing Maria G. Ivanchenko and Molly Megraw 14. Genome-Wide TSS Identification in Maize Maria Katherine Mejia-Guerra, Wei Li, Andrea I. Doseff, and Erich Grotewold 15. Three-Dimensional Multiphoton Imaging of Transcription Factor by ClearSee Yoko Mizuta and Katsutoshi Tsuda 16. Two-Color In Situ Hybridization: A Technique for Simultaneous Detection of Transcripts from Different Loci Taiyo Toriba and Hiro-Yuki Hirano Part IV: Interaction Tests 17. Gene Expression and Transcription Factor Binding Tests Using Mutated-Promoter Reporter Lines Ayako Nishizawa-Yokoi and Nobutoshi Yamaguchi 18. Rapid and Quantitative CELD Assay to Measure the Specificity of Transcription Factor-DNA Binding Interactions and Identify cis-Elements Sundaravelpandian Kalaipandian and Gang-Ping Xue 19. In Situ Proximity Ligation Assay to Detect the Interaction between Plant Transcription Factors and Other Regulatory Proteins Yifeng Xu, Eng-Seng Gan, and Toshiro Ito 20. Cell-Free Protein Synthesis of Plant Transcription Factors Mika Nomoto and Yasuomi Tada Part V: Chromation Analyses 21. Application of MNase-Seq in the Global Mapping of Nucleosome Positioning in Plants Wenli Zhang and Jiming Jiang 22. Genome-Wide Mapping of DNase I Hypersensitive Sites in Tomato Ren Li and Xia Cui 23. Genome-Wide Identification of Chromatin Domains Anchored at the Nuclear Periphery in Plants Xiuli Bi and Chang Liu

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