Plant cell morphogenesis : methods and protocols

書誌事項

Plant cell morphogenesis : methods and protocols

edited by Fatima Cvrčková and Viktor Žárský

(Methods in molecular biology / John M. Walker, series editor, 1992)(Springer protocols)

Humana Press, c2019

2nd ed

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

This book collects techniques to continue exploring post-genomic land plant biology though the wisdom and skills accumulated from work on the founding molecular biology models that can now guide research into other species, including crop plants. Beginning with the visualization of plant cell structures, the volume moves on to cover digital image analysis protocols, qualitative and quantitative detection of the organization and dynamics of individual intracellular structures, the manipulation of intracellular structures, as well as techniques for studying model cell types. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and fully updated, Plant Cell Morphogenesis: Methods and Protocols, Second Edition serves as an ideal source of inspiration for further research into the morphogenesis of plant cells, tissues, and organs.

目次

1. Essential Methods of Plant Sample Preparation for Light Microscopy Ales Soukup and Edita Tylova 2. Selected Simple Methods of Plant Cell Wall Histochemistry and Staining for Light Microscopy Ales Soukup 3. Chemical Fixation, Immunofluorescence, and Immunogold Labelling of Electron Microscopical Sections Ilse Foissner and Margit Hoeftberger 4. Essential Methods of Plant Sample Preparation for High Resolution Scanning Electron Microscopy at Room Temperature Jana Nebesarova 5. Fluorescence Lifetime Imaging of Plant Cell Walls Christine Terryn and Gabriel Paes 6. Raman Spectroscopy in Non-Woody Plants Dorota Borowska-Wykret and Mateusz Dulski 7. Image Analysis: Basic Procedures for Description of Plant Structures Jana Albrechtova, Zuzana Kubinova, Ales Soukup, and Jiri Janacek 8. From Data to Illustrations: Common (Free) Tools for Proper Image Data Handling and Processing Fatima Cvrckova 9. Visualizing and Quantifying In Vivo Cortical Cytoskeleton Structure and Dynamics Amparo Rosero, Denisa Oulehlova, Viktor Zarsky, and Fatima Cvrckova 10. Quantitative and Comparative Analysis of Global Patterns of (Microtubule) Cytoskeleton Organization with CytoskeletonAnalyzer2D Birgit Moeller, Luise Zergiebel, and Katharina Burstenbinder 11. Using FM Dyes to Study Endomembranes and Their Dynamics in Plants and Cell Suspensions Adriana Jelinkova, Katerina Malinska, and Jan Petrasek 12. Transient Gene Expression as a Tool to Monitor and Manipulate the Levels of Acidic Phospholipids in Plant Cells Lise C. Noack, Premysl Pejchar, Juraj Sekeres, Yvon Jaillais, and Martin Potocky 13. The Photo-Convertible Fluorescent Protein Dendra2 Tag as a Tool to Investigate Intracellular Protein Dynamics Alexandra Leskova, Zuzana Kusa, Maria Labajova, Miroslav Krausko, and Jan Jasik 14. Cellular Force Microscopy to Measure Mechanical Forces in Plant Cells Mateusz Majda, Aleksandra Sapala, Anne-Lise Routier-Kierzkowska, and Richard S. Smith 15. Optical Trapping in Plant Cells Tijs Ketelaar, Norbert de Ruijter, and Stefan Niehren 16. Sequential Replicas: Method for In Vivo Imaging of Plant Organ Surfaces that Undergo Deformation Dorota Kwiatkowska, Sandra Natonik-Bialon, and Agata Burian 17. Time-Lapse Imaging of Developing Shoot Meristems Using Confocal Laser Scanning Microscope Olivier Hamant, Pradeep Das, and Agata Burian 18. Quantifying Plant Growth and Cell Proliferation with MorphoGraphX Soeren Strauss, Aleksandra Sapala, Daniel Kierzkowski, and Richard S. Smith 19. Kinematic Characterization of Root Growth by Means of Stripflow Tobias I. Baskin and Ellen Zelinsky 20. Automated Acquisition and Morphological Analysis of Cell Growth Mutants in Physcomitrella patens Giulia Galotto, Jeffrey P. Bibeau, and Luis Vidali 21. Live Cell Imaging of Arabidopsis Root Hairs Tijs Ketelaar 22. Morphological Analysis of Leaf Epidermis Pavement Cells with PaCeQuant Birgit Moeller, Yvonne Poeschl, Sandra Klemm, and Katharina Burstenbinder 23. Imaging of Developing Metaxylem Vessel Elements in Cultured Hypocotyls Takema Sasaki and Yoshihisa Oda 24. Antisense Oligodeoxynucleotide-Mediated Gene Knockdown in Pollen Tubes Martin Potocky, Radek Bezvoda, and Premysl Pejchar 25. Plant Cell Lines in Cell Morphogenesis Research: From Phenotyping to -Omics Petr Klima, Vojtech Cermak, Miroslav Srba, Karel Muller, Jan Petrasek, Josef Sonka, Lukas Fischer, and Zdenek Opatrny

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