Plant cell morphogenesis : methods and protocols
著者
書誌事項
Plant cell morphogenesis : methods and protocols
(Methods in molecular biology / John M. Walker, series editor, 1992)(Springer protocols)
Humana Press, c2019
2nd ed
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This book collects techniques to continue exploring post-genomic land plant biology though the wisdom and skills accumulated from work on the founding molecular biology models that can now guide research into other species, including crop plants. Beginning with the visualization of plant cell structures, the volume moves on to cover digital image analysis protocols, qualitative and quantitative detection of the organization and dynamics of individual intracellular structures, the manipulation of intracellular structures, as well as techniques for studying model cell types. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and fully updated, Plant Cell Morphogenesis: Methods and Protocols, Second Edition serves as an ideal source of inspiration for further research into the morphogenesis of plant cells, tissues, and organs.
目次
1. Essential Methods of Plant Sample Preparation for Light Microscopy
Ales Soukup and Edita Tylova
2. Selected Simple Methods of Plant Cell Wall Histochemistry and Staining for Light Microscopy
Ales Soukup
3. Chemical Fixation, Immunofluorescence, and Immunogold Labelling of Electron Microscopical Sections
Ilse Foissner and Margit Hoeftberger
4. Essential Methods of Plant Sample Preparation for High Resolution Scanning Electron Microscopy at Room Temperature
Jana Nebesarova
5. Fluorescence Lifetime Imaging of Plant Cell Walls
Christine Terryn and Gabriel Paes
6. Raman Spectroscopy in Non-Woody Plants
Dorota Borowska-Wykret and Mateusz Dulski
7. Image Analysis: Basic Procedures for Description of Plant Structures
Jana Albrechtova, Zuzana Kubinova, Ales Soukup, and Jiri Janacek
8. From Data to Illustrations: Common (Free) Tools for Proper Image Data Handling and Processing
Fatima Cvrckova
9. Visualizing and Quantifying In Vivo Cortical Cytoskeleton Structure and Dynamics
Amparo Rosero, Denisa Oulehlova, Viktor Zarsky, and Fatima Cvrckova
10. Quantitative and Comparative Analysis of Global Patterns of (Microtubule) Cytoskeleton Organization with CytoskeletonAnalyzer2D
Birgit Moeller, Luise Zergiebel, and Katharina Burstenbinder
11. Using FM Dyes to Study Endomembranes and Their Dynamics in Plants and Cell Suspensions
Adriana Jelinkova, Katerina Malinska, and Jan Petrasek
12. Transient Gene Expression as a Tool to Monitor and Manipulate the Levels of Acidic Phospholipids in Plant Cells
Lise C. Noack, Premysl Pejchar, Juraj Sekeres, Yvon Jaillais, and Martin Potocky
13. The Photo-Convertible Fluorescent Protein Dendra2 Tag as a Tool to Investigate Intracellular Protein Dynamics
Alexandra Leskova, Zuzana Kusa, Maria Labajova, Miroslav Krausko, and Jan Jasik
14. Cellular Force Microscopy to Measure Mechanical Forces in Plant Cells
Mateusz Majda, Aleksandra Sapala, Anne-Lise Routier-Kierzkowska, and Richard S. Smith
15. Optical Trapping in Plant Cells
Tijs Ketelaar, Norbert de Ruijter, and Stefan Niehren
16. Sequential Replicas: Method for In Vivo Imaging of Plant Organ Surfaces that Undergo Deformation
Dorota Kwiatkowska, Sandra Natonik-Bialon, and Agata Burian
17. Time-Lapse Imaging of Developing Shoot Meristems Using Confocal Laser Scanning Microscope
Olivier Hamant, Pradeep Das, and Agata Burian
18. Quantifying Plant Growth and Cell Proliferation with MorphoGraphX
Soeren Strauss, Aleksandra Sapala, Daniel Kierzkowski, and Richard S. Smith
19. Kinematic Characterization of Root Growth by Means of Stripflow
Tobias I. Baskin and Ellen Zelinsky
20. Automated Acquisition and Morphological Analysis of Cell Growth Mutants in Physcomitrella patens
Giulia Galotto, Jeffrey P. Bibeau, and Luis Vidali
21. Live Cell Imaging of Arabidopsis Root Hairs
Tijs Ketelaar
22. Morphological Analysis of Leaf Epidermis Pavement Cells with PaCeQuant
Birgit Moeller, Yvonne Poeschl, Sandra Klemm, and Katharina Burstenbinder
23. Imaging of Developing Metaxylem Vessel Elements in Cultured Hypocotyls
Takema Sasaki and Yoshihisa Oda
24. Antisense Oligodeoxynucleotide-Mediated Gene Knockdown in Pollen Tubes
Martin Potocky, Radek Bezvoda, and Premysl Pejchar
25. Plant Cell Lines in Cell Morphogenesis Research: From Phenotyping to -Omics
Petr Klima, Vojtech Cermak, Miroslav Srba, Karel Muller, Jan Petrasek, Josef Sonka, Lukas Fischer, and Zdenek Opatrny
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