Fasciola hepatica : methods and protocols
著者
書誌事項
Fasciola hepatica : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 2137）（Springer protocols）
Humana Press, c2020
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This volume described basic and advanced protocols to study F. hepatica parasite biology. Chapters guide readers through protocols on different developmental stages of F. hepatica, characterize tegumental, secreted proteins, spatial and temporal gene expression, immunological effects of ESP on macrophages, eosinophil apoptosis, macrophages alternative activation, toll-like receptor interactions, obtain peritoneal and splenic dendritic cells of mice, and protocols to detect F. hepatica albendazole resistance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Fasciola hepatica: Methods and Protocols provides different topics in areas such a biochemistry, immunology, molecular biology, microscopy, vaccinology in order to extend the interest in this book to research community working with Fasciola hepatica and related trematodes.
目次
1. Maintenance of Life Cycle at the Laboratory
Valeria Gayo, Martin Cancela, and Daniel Acosta
2. Microscopical Techniques to Analyse the Hepatic and Peritoneal Changes Caused by Fasciola hepatica Infection
M Teresa Ruiz-Campillo, Veronica Molina-Hernandez, Jose Perez, Isabel L Pacheco, Raul Perez, Alejandro Escamilla, Javier F Martinez-Moreno, Alvaro Martinez-Moreno, and Rafael Zafra
3. Isolation of Secreted and Tegumental Surface Proteins from Fasciola hepatica
Eduardo de la Torre-Escudero and Mark W. Robinson
4. Isolation and Analysis of Fasciola hepatica Extracellular Vesicles
Alicia Galiano, Maria Teresa Minguez, Christian M. Sanchez, and Antonio Marcilla
5. Cloning and Heterologous Expression of Protein-Coding Sequences in Escherichia coli
Martin Cancela and Gabriela Maggioli
6. Gene Silencing in the Liver Fluke Fasciola hepatica: RNA Interference
Gabriel Rinaldi, Nicolas Dell'Oca, Estela Castillo, and Jose F. Tort
7. Analysis of Gene Expression in Fasciola hepatica Juveniles and Adults by In Situ Hybridization
Estela Castillo and Uriel Koziol
8. Evasion of Host Immunity during Fasciola hepatica Infection
Robin J Flynn and Mayowa Musah-Eroje
9. Immunomodulatory Effect of Fasciola hepatica Excretory-secretory Products on Macrophages
Lorena Guasconi, Marianela Serradell, Diana T. Masih, and Laura S Chiapello
10. Study of Eosinophil Apoptosis Induced by Fasciola hepatica Excretory-secretory Products
Marianela C. Serradell, Lorena Guasconi, Laura A. Cervi, Laura S. Chiapello, and Diana T. Masih
11. Purification of native Fasciola hepatica Fatty Acid Binding Protein and the Induction of Alternative Activation of Human Macrophages
Olgary Figueroa-Santiago
12. Possible Role for Toll-Like Receptors in Interaction of Fasciola hepatica Excretory-Secretory Products with Human Monocyte Cell Line
Olgary Figueroa-Santiago
13. Evaluation of the Immune Regulatory Properties of Dendritic Cells during Fasciola hepatica Infection
Teresa Freire
14. Design of a Peptide-carrier Vaccine Based on the Highly Immunogenic Fasciola hepatica Leucine Aminopeptidase
Cecilia Salazar, Jose F Tort, and Carlos Carmona
15. Liver Fluke Vaccine Assessment in Cattle
Gabriela Maggioli, Cecilia Salazar, Federico Fossa, and Carlos Carmona
16. Testing Albendazole Resistance in Fasciola hepatica
Luis I. Alvarez, Maria Martinez Valladares, Candela Canton, Carlos E. Lanusse, and Laura Ceballos
17. Drug Targets: Screening for Small Molecules that Inhibit Fasciola hepatica Enzymes
Florencia Ferraro, Mauricio A. Cabrera, Guzman I. Alvarez, and Ileana Corvo
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