Phytoplasmas : methods and protocols

著者

• Musetti, Rita
• Pagliari, Laura

書誌事項

Phytoplasmas : methods and protocols

edited by Rita Musetti and Laura Pagliari

（Methods in molecular biology, 1875）（Springer protocols）

Humana Press, c2019

注記

Includes bibliographical references and index

内容説明・目次

内容説明

This book presents a set of modern protocols forming a solid background for who want to start or improve research programme on phytoplasmas. Chapters guide readers through detailed techniques for maintaining phytoplasma collections, border inspection, detection of different phytoplasma strains, new pipelines to produce phytoplasma genome draft, protocols for phytoplasma gene expression analyses, and methods for the investigation of the phloem tissue. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Phytoplasmas: Methods and Protocols aims to ensure successful results in the further study of this vital field.

目次

1. Phytoplasmas: An Introduction L. Pagliari and R. Musetti Part II: Preparing Plant Material 2. Micro-tom Tomato Grafting for Stolbur-phytoplasma Transmission: Different Grafting Techniques Sara Buoso and Alberto Loschi 3. Phytoplasma Transmission: Insect Rearing and Infection Protocols L. Pagliari, J. Chuche, D. Bosco, and D. Thiery 4. Sampling Methods for Leafhopper, Planthopper, and Psyllid Vectors Kerstin Kruger and Nicola Fiore 5. Symptoms of Phytoplasma Diseases Paolo Ermacora and Ruggero Osler Part II: Molecular Analyses 6. Comparison of Different Procedures for DNA Extraction for Routine Diagnosis of Phytoplasmas Carmine Marcone 7. Standard Detection Protocol: PCR and RFLP Analyses Based on 16S rRNA Gene Assunta Bertaccini, Samanta Paltrinieri and Nicoletta Contaldo 8. PCR-based Sequence Analysis on Multiple Genes other than 16S rRNA Gene for Differentiation of Phytoplasmas Marta Martini, Kristi D. Bottner-Parker, and Ing-Ming Lee 9. Real-time PCR Protocol for Phytoplasma Detection and Quantification Yusuf Abou-Jawdah, Vicken Aknadibossian, Maan Jawhari, Patil Tawidian, and Peter Abrahamian 10. Duplex TaqMan Real-Time PCR for Rapid Quantitative Analysis of a Phytoplasma in its Host Plant without External Standard Curves Sanja Baric 11. A Multiplex-PCR Method for Diagnosis of AY-Group Phytoplasmas Shigeyuki Kakizawa 12. One-step Multiplex Quantitative RT-PCR for the Simultaneous Detection of Viroids and Phytoplasmas Ioanna Malandraki, Christina Varveri and Nikon Vassilakos 13. A Rapid Protocol of Crude RNA/DNA Extraction for RT-qPCR Detection and Quantification Claudio Ratti, Stefano Minguzzi, and Massimo Turina 14. Quantitative Analysis with Droplet Digital PCR Natasa Mehle and Tanja Dreo 15. Rapid Sample Preparation and LAMP for Phytoplasma Detection Jennifer Hodgetts 16. Assembly of Phytoplasma Genome Drafts from Illumina Reads using Phytoassembly Cesare Polano and Giuseppe Firrao 17. Protocol for the Definition of a Multi-Spectral Sensor for Specific Foliar Disease Detection: Case of 'Flavescence Doree' H. Al-Saddik , A. Laybros, JC. Simon, and F. Cointault 18. Transcriptomic Analyses of Phytoplasmas Davide Pacifico, Simona Abba, and Sabrina Palmano Part III: Site-specific Analyses 19. Transcriptomic Analyses of Phytoplasmas Davide Pacifico, Simona Abba, and Sabrina Palmano 20. Laser Microdissection of Phytoplasma-infected Grapevine Leaf Phloem Tissue for Gene Expression Study Simonetta Santi 21. Collection of Phloem Sap in Phytoplasma-infected Plants Matthias R. Zimmermann,Torsten Knauer and, Alexandra C.U. Furch 22. DAPI and Confocal Laser-Scanning Microscopy for in vivo Imaging of Phytoplasmas Rita Musetti and Stefanie Vera Buxa 23. Immunofluorescence Assay to Study Early Events of Vector Salivary Gland Colonization by Phytoplasmas Luciana Galetto, Marta Vallino, Mahnaz Rashidi, and Cristina Marzachi Part IV: Plant-pathogen Interaction 24. Characterization of Phytoplasmal Effector Protein Interaction with Proteinaceous Plant Host Targets using Bimolecular Fluorescence Complementation (BiFC) Katrin Janik, Hagen Stellmach, Cecilia Mittelberger, and Bettina Hause 25. Collection, Identification, and Statistical Analysis of Volatile Organic Compound Patterns Emitted by Phytoplasma Infected Plants Jurgen Gross, Jannicke Gallinger, and Margit Rid 26. Quantification of Phytohormones by HPLC-MS/MS Including Phytoplasma-infected Plants Marilia Almeida-Trapp and Axel Mithoefer

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詳細情報

• NII書誌ID(NCID): BB31576281
• ISBN:
  • 9781493988365
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: New York
• ページ数/冊数: xi, 362 p.
• 大きさ: 26 cm
• 親書誌ID:
  • BA07889873
  • BA88873632