The plant cell wall : methods and protocols
著者
書誌事項
The plant cell wall : methods and protocols
(Methods in molecular biology / John M. Walker, series editor, 2149)
Humana Press, c2020
2nd ed.
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注記
Includes index
内容説明・目次
内容説明
This second edition volume expands on the previous edition with many new and updated chapters discussing the latest techniques used to investigate cell wall biochemistry, biomechanical properties, chemistry, and biology. Chapters in this book also cover topics such as cell wall composition and structure, plant tissue culture protoplast isolation, genetic manipulation, investigation of enzyme activities, and in situ localization of wall components. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, The Plant Cell Wall: Methods and Protocols, Second Edition is a valuable resource for both novice and expert scientists interested in learning more about this field.
目次
Preface...Acknowledgments...Table of Contents...Contributing Authors...
1. High-Voltage Paper Electrophoresis (HVPE)Stephen C. Fry
2. Carbohydrate Gel ElectrophoresisFlorence Goubet, Paul Dupree, and Katja Salomon Johansen
3. Capillary Electrophoresis with Detection by Laser-Induced FluorescenceAndrew Mort and Xiangmei Wu
4. Theory and Practice in Measuring In Vitro Extensibility of Growing Plant Cell WallsDaniel J. Cosgrove
5. Formation of Cellulose-Based Composites with Hemicelluloses and Pectins using Komagataeibacter FermentationDeirdre Mikkelsen, Patricia Lopez-Sanchez, Dongjie Wang, and Michael J. Gidley
6. Plant Tissue CulturesAnna Karkoenen, Arja Santanen, Kuninori Iwamoto, and Hiroo Fukuda
7. Protoplast Isolation and Manipulation in the Unicellular Model Plant, Penium margaritaceumDavid S. Domozych, Eleanore Ritter, Anna Lietz, Berke Tinaz, and Sandra C. Raimundo
8. Knocking Out the Wall: Revised Protocols for Gene Targeting in Physcomitrella patensAlison W. Roberts, Christos S. Dimos, Michael J. Budziszek, Jr., Chessa A. Goss, Virginia Lai, and Arielle M. Chaves
9. Expression of Cell-Wall Modifying Enzymes in Aspen for Improved Lignocellulose ProcessingMarta Derba-Maceluch and Ewa J. Mellerowicz
10. Activity and Action of Cell-Wall TransglycanasesLenka Frankova and Stephen C. Fry
11. Screening for Cellulolytic Plant Enzymes using Colorimetric and Fluorescence MethodsKirsten Krause and Stian Olsen
12. Using Solid-State 13C NMR Spectroscopy to Study the Molecular Organization of Primary Plant Cell WallsStefan J. Hill, Tracey J. Bootten, Laurence D. Melton, and Philip J. Harris
13. High-Resolution Imaging of Cellulose Organization in Cell Walls by Field Emission Scanning Electron MicroscopyYunzhen Zheng, Gang Ning, and Daniel J. Cosgrove
14. Analyzing Plant Cell Wall Ultrastructure by Scanning Near-Field Optical Microscopy (SNOM)Tobias Keplinger and Ingo Burgert
15. Raman Imaging of Plant Cell WallsBatirtze Prats Mateu, Peter Bock, and Notburga Gierlinger
16. Analysis of Plant Cell Walls by Attenuated Total Reflectance Fourier Transform Infrared SpectroscopyRicardo M. F. da Costa, William Barrett, Jose Carli, and Gordon G. Allison
17. Rapid Assessment of Cell Wall Polymer Distribution and Surface Topology of Arabidopsis SeedlingsMary L. Tierney, Li Sun, and David S. Domozych
18. Analysis of Plant Cell Walls using High-Throughput Profiling Techniques with Multivariate MethodsJohn P. Moore, Yu Gao, Anscha J.J. Zietsman, Jonatan U. Fangel, Johan Trygg, William G.T. Willats, and Melane A. Vivier
19. Vibratome Sectioning of Plant Materials for (Immuno)Cytochemical StainingOlivier Leroux
20. Monoclonal Antibodies, Carbohydrate-Binding Modules, and the Detection of Polysaccharides in Cell Walls from Plants and Marine AlgaeDelphine Duffieux, Susan E. Marcus, J. Paul Knox, and Cecile Herve
21. Electron Tomography and Immunogold Labeling as Tools to Analyze de novo Assembly of Plant Cell WallsMarisa S. Otegui
22. Contributions to Arabinogalactan Proteins AnalysisRomain Castilleux, Marc Ropitaux, Youssef Manasfi, Sophie Bernard, Maite Vicre-Gibouin, and Azeddine Driouich
23. In Situ/Subcellular Localization of Arabinogalactan Protein Expression by Fluorescent In Situ Hybridization, FISHMario Luis da Costa, Maria-Teresa Solis, Pilar S. Testillano, and Silvia Coimbra
24. Localization, Extraction, and Quantification of Plant and Algal Arabinogalactan ProteinsReina J. Veenhof and Zoe A. Popper
25. Plant Cell Wall Proteomes: Bioinformatics and Cell Biology Tools to Assess the Bona Fide Cell Wall Localization of ProteinsDavid Roujol, Laurent Hoffmann, Helene San Clemente, Corinne Schmitt-Keichinger, Christophe Ritzenthaler, Vincent Burlat, and Elisabeth Jamet
26. Bioinformatic Identification of Plant Hydroxyproline-Rich GlycoproteinsXiao Liu, Savannah McKenna, Lonnie R. Welch, and Allan M. Showalter
27. Bioinformatics Analysis of Plant Cell Wall EvolutionElisabeth Fitzek, Rhiannon Balazic, and Yanbin Yin
28. Automated Glycan Assembly of Plant Cell Wall OligosaccharidesFabian Pfrengle
29. Computerized Molecular Modelling of CarbohydratesAlfred D. French and Glenn P. Johnson
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