Glucose transport : methods and protocols
Author(s)
Bibliographic Information
Glucose transport : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1713）（Springer protocols）
Humana Press, 2017
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Description and Table of Contents
Description
This volume explores methods used to examine the structure-function relationship of glucose transporters both in vitro and ex vivo. Chapters in this book cover topics such as expression and purification of glucose transporters in heterologous expression systems, crystal structure determination, glucose transport activity assays in cellular and synthetic model systems, investigation of the GLUT4 membrane trafficking machinery by novel labeling strategies combined with advanced fluorescence microscopy methods, and tracking GLUT2 translocation by live cell imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Glucose Transport: Methods and Protocols is a valuable resource that helps researchers with determining specific roles of different GLUTs in various organisms.
Table of Contents
Preface...
Table of Contents...
Contributing Authors...
1 Expression and Purification of Rat Glucose Transporter 1
in Pichia pastoris
Raminta Venskutonyte, Karin Elbing, and Karin Lindkvist-Petersson
2 Crystallization and Structural Determination of the Human Glucose Transporters GLUT1 and GLUT3
Dong Deng and Nieng Yan
3 Screening and Scale-Up of GLUT Transporter Constructs Suitable for Biochemical and Structural Studies
Gregory Verdon, Hae Joo Kang, and David Drew
4 GLUT Characterization using Frog Xenopus laevis Oocytes
Wentong Long, Debbie O'Neill, and Chris I. Cheeseman
5 Glucose Uptake in Heterologous Expression Systems
Eunice E. Lee and Richard C. Wang
6 Evaluating the Impact of GLUT Inhibition on Cellular Bioenergetics Using a Seahorse XF Analyzer
Changyong Wei, Monique Heitmeir, Paul W. Hruz, and Mala Shanmugam
7 Glucose Transport Activity Measured in Giant Vesicles
Jesper Sondergaard Hansen and Karin Lindkvist-Petersson
8 Design, Synthesis, and Evaluation of GLUT Inhibitors
Carlotta Granchi, Tiziano Tuccinardi, and Filippo Minutolo
9 Applying Microfluidic Systems to Study Effects of Glucose at Single Cell Level
Niek Welkenhuysen, Caroline B. Adiels, Mattias Goksoer, and Stefan Hohmann
10 A Growth-Based Screening System for Hexose Transporters in Yeast
Eckhard Boles and Mislav Oreb
11 Identification of Insulin Activated Rab Proteins in Adipose Cells Using Bio-ATB-GTP Photolabeling Technique
Francoise Koumanov and Geoffrey D. Holman
12 Total Internal Reflection Fluorescence Microscopy to Study GLUT4 Trafficking
Sebastian Wasserstrom, Bjoern Moren, and Karin G. Stenkula
13 Translocation and Redistribution of GLUT4 Using a Dual-Labeled Reporter Assay
Robert M. Jackson and Ann Louise Olson
14 GLUT4 Translocation in Single Muscle Cells in Culture: Epitope Detection by Immunofluorescence
Javier R. Jaldin-Fincati, Philip J. Bilan, and Amira Klip
15 Glucose Transport: Methods for Interrogating GLUT4 Trafficking in Adipocytes
Dougall M. Norris, Tom A. Geddes, David E. James, Daniel J. Fazakerley, and James G. Burchfield
16 Proximity Ligation Assay to Study the GLUT4 Membrane Trafficking Machinery
Dimitrios Kioumourtzoglou, Gwyn W. Gould, and Nia J. Bryant
17 Quantification of Cell Surface Glucose Transporters in the Heart Using a Biotinylated Photolabeled Assay
Zahra Maria and Veronique A. Lacombe
18 Tracking GLUT2 Translocation by Live Cell Imaging
Sabina Tsytkin-Kirschenzweig, Merav Cohen, and Yaakov Nahmias
19 GLUT2-Expressing Neurons as Glucose Sensors in the Brain: Electrophysiological Analysis
Gwenael Labouebe, Bernard Thorens, and Christophe Lamy
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