The LINC complex : methods and protocols
著者
書誌事項
The LINC complex : methods and protocols
（Methods in molecular biology / John M. Walker, series editor, 1840）（Springer protocols）
Humana Press, c2018
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This volume describes research methodologies and approaches used to study the Linker of Nucleoskeleton and Cytoskeleton (LINC) complex and its cellular functions. Chapters detail structural and biochemical analysis of LINC complexes, mechanical aspects of the LINC complex, analysis of the LINC complex in model systems and development, and LINC complex in mammalian tissue, organs, and disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, The LINC Complex: Methods and Protocols aims to ensure successful results in the further study of this vital field.
目次
Part 1 - Structural and Biochemical Analysis of LINC complexes
1. EM Tomography of LINC Complexes
Marie-Christin Spindler, Frederick Helmprobst, Christian Stigloher and Ricardo Benavente
2. Recombinant Purification of the Periplasmic Portion of the LINC Complex
Victor E. Cruz and Thomas U. Schwartz
3. Analysis of High Molecular Weight Isoforms of Nesprin-1 and Nesprin-2 with Vertical Agarose Gel Electrophoresis
Chloe Potter and Didier Hodzic
4. Analysis of Nesprin-2 Interaction with its Binding Partners and Actin
Susumu Antoku and Gregg G. Gundersen
5. Interactions of Nesprin-4-containing LINC Complexes in Outer Hair Cells Explored by BioID
Brian Burke
Part 2 - Mechanical Aspects of the LINC Complex
6. Using Nesprin Tension Sensors to Measure Force on the LINC Complex
Paul T. Arsenovic and Daniel E. Conway
7. Analyzing Mechanotransduction through the LINC Complex in Isolated Nuclei
Nejma Belaadi, Angelique Millon-Fremillon, Julien Aureille, and Christophe Guilluy
8. Direct Force Probe for Nuclear Mechanics
Vincent J. Tocco, Srujana Neelam, Qiao Zhang, Richard B. Dickinson, and Tanmay P. Lele
9. Centrifugal Displacement of Nuclei in Adherent Cells to Study LINC Complex Dependent Mechanisms of Homeostatic Nuclear Positioning
Ruijun Zhu and Gregg G. Gundersen
10. Assembly and Use of a Microfluidic Device to Study Cell Migration in Confined Environments
Jeremy Keys, Aaron Windsor, and Jan Lammerding
Part 3 - Analysis of the LINC Complex in Model Systems and Development
11. Investigating LINC Complex Protein Homo-oligomerization in the Nuclear Envelopes of Living Cells Using Fluorescence Fluctuation Spectroscopy
Jared Hennen, Isaac Angert, Kwang-Ho Hur, G.W. Gant Luxton, and Joachim D. Mueller
12. Functional Analysis of the Yeast LINC Complex Using Fluctuation Spectroscopy and Super Resolution Imaging
Jay R. Unruh, Brian D. Slaughter, and Sue L. Jaspersen
13. Genetic Analysis of Nuclear Migration and Anchorage to Study LINC Complexes During Development of Caenorhabditis elegans
Heidi N. Fridolfsson, Leslie A. Herrera, James N. Brandt, Natalie E. Cain, Greg J. Hermann, and Daniel A. Starr
14. High-resolution Imaging Methods to Analyze LINC Complex Function During Drosophila Muscle Development
Alexander L. Auld, Mary Ann Collins, Torrey R. Mandigo, and Eric S. Folker
15. Computational Methods for Studying the Plant Nucleus
Axel Poulet, Xiao Zhou, Kentaro Tamura, Iris Meier, Christophe Tatout, Katja Graumann, and David E. Evans
16. Investigation of Nuclear Periphery Protein Interactions in Plants Using the Membrane Yeast Two-Hybrid (MbY2H) System
Maxime Voisin, Emmanuel Vanrobays, and Christophe Tatout
17. Immunolabeling Protocols for Studying Meiosis in Plant Mutants Defective for Nuclear Envelope Components
Javier Varas and Monica Pradillo
Part 4 - LINC Complex in Mammalian Tissue, Organs and Disease
18. Generation and Analysis of Striated Muscle Selective LINC Complex Protein Mutant Mice
Matthew J Stroud, Xi Fang, Jennifer Veevers, and Ju Chen
19. An in vitro System to Measure the Positioning, Stiffness and Rupture of the Nucleus in Skeletal Muscle
William Roman, Mafalda R. Pimentel, and Edgar R. Gomes
20. Functional Analysis of LINC Complexes in Skin
Iakowos Karakesisoglou, Carmen Mross, and Angelika A. Noegel
21. Detection of SUN1 Splicing Variants at the mRNA and Protein Levels in Cancer
Ayaka Matsumoto, Nariaki Matsuura, and Miki Hieda
22. Next-generation Sequencing and Mutational Analysis: Implications for Genes Encoding LINC Complex Proteins
Peter L. Nagy and Howard J. Worman
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